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The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论:四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用;四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖,可能是各种单味药多糖综合作用的结果;多糖可能是四君子汤"益气健脾"药效的主要成分;小肠上皮细胞可能是四君子汤总多糖的作用靶点之一,提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

New ideas and methods in the paper:1 The formulas on perforated casing collapse strength were deducted, which offereda convenient method to analyze the relationship between casing collapse strength and perforation;2 The stress and displacement formulas on the system of casing, cement anddeformation under the non-uniform load were deducted, which offered the theoretical found for designing optimally casing string;3 The relationship between structure in-situ field of whole oil field and casing failurewere studied, which offered the scheme for the prediction of casing failure. The process of study in the paper:1 For simulating the relationship between the structure in-situ field and the casingfailure in the oil-deformation, the tests of the uniaxial tensile, compress, tnaxial compress and dilatability were made on the samples of the drill cores from deformation of casing failure position in Yuejing II oil field.

本文的主要创新: 1推导出了射孔套管抗挤强度的解析式,为分析射孔与套管强度的变化关系提供了简便的方法; 2推导出了非均匀地层载荷作用下套管、水泥环和地层组合体的应力和位移解析式,并非均匀作用下套管柱优化设计提供了理论依据; 3研究整个油田区块构造应力场与套管损坏关系,为预测套管损坏提供了可行方案;本文的研究过程如下: 1为模拟构造应力场与油层段套管损坏关系,做了大量的岩石单轴抗拉、单轴抗压、三轴试验以及岩石遇水膨胀试验,获得了研究区块岩石的弹性模量、泊松比、内聚力、内摩擦角以及岩石的线膨胀系数,这此数据为本文研究油层段套管损坏机理研究的理论分析和有限元法提供了实验数据。

Ascorbate is concomitantly oxidized to monodehydroascorbate. Some monodehydroascorbate radicals are re-reduced in a reaction catalyzed by monodehy- droascorbate reductase, but the remainder undergoes spontaneous dismutation to ascorbate and dehydroascorbate.

在抵抗氧化胁迫的反应中,抗坏血酸自身被氧化为单脱氢抗坏血酸,一部分单脱氢抗坏血酸在单脱氢抗坏血酸还原酶的催化作用下还原为抗坏血酸,另一部分单脱氢抗坏血酸的在歧化作用下被不成比例地还原为抗坏血酸或氧化为脱氢抗坏血酸。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Till now, the most used method for storage of hydrogen in array of SWNT's is by physisorption inside the SWNT's and on the outer surface of the SWNT's or in the interstice between SWNT's.

到目前为止,在单壁碳纳米管阵列中贮存氢的最常用的方法是通过虹吸作用或电化学作用使氢物理吸附在单壁碳纳米管的内部、外表面上或者单壁碳纳米管之间的间隙中。

Both the interactions of haplotype vs. food (R0: P=0.038; rm: P=0.027) and that of haplotype vs. temperature (R0: P=0.006; rm: P=0.000) had obvious effect on growth reponses of the clonal groups. Moreover, there was significant interaction of haplotype vs. temperature (lorica length: P=0.033; lorica width: P=0.000) and haplotype vs. temperature (lorica length: P=0.027; lorica width: P=0.000) on body size of the clonal group.

生命表实验结果显示,单倍型与食物浓度的交互作用(R0:P=0.038;rm:P=0.027)以及单倍型与温度的交互作用(R0:P=0.006;rm:P=0.000)对种群增长均有显著影响,这4个单倍型克隆群对环境条件具有不同的偏好,并且各个克隆群对于环境变化有不同的生殖反应。

Dealcoholization temperature markedly influences the surface area and porosity of the resultant MAO-supports. When the support was pretreated at lower or higher temperature, the corresponding MAO-support shows very low surface area;2.XRD and IR analyses indicate that MAO has reacted with the residual ethanol in the supports, which is an important manner to get MAO fixed on the carrier. Morever, XPS analyse indicates that MAO can also be coordinated with MgCl_2, which is another way to get MAO fixed on the support;3.ICP and EDX analyses indicate that dealcoholization temperature not only determined the element content on the solid catalyst, but also influences the element distribution on the carrier;4.The activities of the supported phenoxy-imine catalysts and the properties of resultant polymers are strongly dependent on the dealcoholization temperature. The support (MSP-5) obtained by treating MgCl_2·2.56C_2H_5OH at 160℃for 4 h, then modified by MAO is very effective for immobilizing complex 3, the resultant solid catalyst (MSPC-5) shows very high activity in ethylene polymerization, and its kinetics of polymerization is stable during the reaction process. Finally, PEs with spherical morphology and high bulk density (over 0.35g/ml) were obtained, without reactor fouling;5.In this work, polymerization conditions such as alkylaluminums, Al/Zr ratio, temperature and H_2 had a pronounced effect on the activity of MSPC-5 and properties of PE;(3). New MgCl_2-Supported Single-Site Catalysts for Ethylene PolymerizationIn this work, a kind of new MgCl_2 support was obtained by anhydrous MgCl_2 co-milled with solid MAO, and it is a creative contribution. Then some single-site catalysts were supported on the new MgCl_2 support, and the resulted solid catalysts were tested in ethylene polymerization, the results indicated that:1.XRD and IR analyses indicate that MAO does be coordinated with MgCl_2, which is in good agreement with the results obtained by XPS;2.co-mlling time had no obvious effect on the texture of support after 12 hours;3.In this work, MgCl_2 was co-milled with solid MAO for different hours, and then complex 3 was supported on these co-milled supports. It was found that co-milling time markedly influences the activity of solid catalysts, but it had negligible effect on the kinetic profile and the properties of resultant PE.

XPS研究结果表明,MAO不仅仅只和乙醇作用,MAO还与MgCl_2本身有一定的作用,这是一个极为重要的发现;3、通过ICP和EDX表征,发现载体的脱醇温度直接影响着元素在固体催化剂中的含量及分布;4、脱醇温度极大地影响着负载苯氧基亚胺类催化剂的活性和聚合物的性能,特别是原始载体经160℃活化4小时制备得到的固体催化剂MSPC-5的活性明显高于其他脱醇温度条件下制得的固体催化,并且催化剂寿命长,表现出稳定的动力学行为,最终获得了高堆密度(大于0.35g/ml)的球形聚乙烯颗粒;5、本文选择MSPC-5为研究对象,考察了聚合反应参数对催化剂的活性及其聚合物的影响,研究发现:不同的烷基铝对催化剂具有非常重要的影响,特别足TIBA对MSPC-5的助催化活性最高,烷基铝的加入量有一个较佳值,聚合温度为80度时活性最高,H_2的加入使催化剂的活性明显降低且聚合物的分子量也减小;、新型氯化镁载体负载单活性中心催化剂催化乙烯聚合本文创造性采用固体MAO与无水氯化镁直接进行共研磨,制备出了一种的新型的氯化镁载体,并将该载体用于多种单活性中心催化剂的负载化研究,研究表明:1、通过BET、XRD的表征,再次证实了MAO与MgCl_2之间具有直接作用,与XPS的研究结果相一致;2、当研磨时间高于12小时时,延长研磨时间对载体的结构没有显著的影响;3、将配合物3负载于共研磨时间不同的一系列载体上,乙烯聚合结果表明,尽管共研磨时间对负载催化剂的活性具有明显的影响,但是它对聚合物的性能以及催化剂的影响不明显。

Dealcoholization temperature markedly influences the surface area and porosity of the resultant MAO-supports. When the support was pretreated at lower or higher temperature, the corresponding MAO-support shows very low surface area; 2.XRD and IR analyses indicate that MAO has reacted with the residual ethanol in the supports, which is an important manner to get MAO fixed on the carrier. Morever, XPS analyse indicates that MAO can also be coordinated with MgCl_2, which is another way to get MAO fixed on the support; 3.ICP and EDX analyses indicate that dealcoholization temperature not only determined the element content on the solid catalyst, but also influences the element distribution on the carrier; 4.The activities of the supported phenoxy-imine catalysts and the properties of resultant polymers are strongly dependent on the dealcoholization temperature. The support (MSP-5) obtained by treating MgCl_2·2.56C_2H_5OH at 160℃for 4 h, then modified by MAO is very effective for immobilizing complex 3, the resultant solid catalyst (MSPC-5) shows very high activity in ethylene polymerization, and its kinetics of polymerization is stable during the reaction process. Finally, PEs with spherical morphology and high bulk density (over 0.35g/ml) were obtained, without reactor fouling; 5.In this work, polymerization conditions such as alkylaluminums, Al/Zr ratio, temperature and H_2 had a pronounced effect on the activity of MSPC-5 and properties of PE;(3). New MgCl_2-Supported Single-Site Catalysts for Ethylene Polymerization In this work, a kind of new MgCl_2 support was obtained by anhydrous MgCl_2 co-milled with solid MAO, and it is a creative contribution. Then some single-site catalysts were supported on the new MgCl_2 support, and the resulted solid catalysts were tested in ethylene polymerization, the results indicated that: 1.XRD and IR analyses indicate that MAO does be coordinated with MgCl_2, which is in good agreement with the results obtained by XPS; 2.co-mlling time had no obvious effect on the texture of support after 12 hours; 3.In this work, MgCl_2 was co-milled with solid MAO for different hours, and then complex 3 was supported on these co-milled supports. It was found that co-milling time markedly influences the activity of solid catalysts, but it had negligible effect on the kinetic profile and the properties of resultant PE.

XPS研究结果表明,MAO不仅仅只和乙醇作用,MAO还与MgCl_2本身有一定的作用,这是一个极为重要的发现; 3、通过ICP和EDX表征,发现载体的脱醇温度直接影响着元素在固体催化剂中的含量及分布; 4、脱醇温度极大地影响着负载苯氧基亚胺类催化剂的活性和聚合物的性能,特别是原始载体经160℃活化4小时制备得到的固体催化剂MSPC-5的活性明显高于其他脱醇温度条件下制得的固体催化,并且催化剂寿命长,表现出稳定的动力学行为,最终获得了高堆密度(大于0.35g/ml)的球形聚乙烯颗粒; 5、本文选择MSPC-5为研究对象,考察了聚合反应参数对催化剂的活性及其聚合物的影响,研究发现:不同的烷基铝对催化剂具有非常重要的影响,特别足TIBA对MSPC-5的助催化活性最高,烷基铝的加入量有一个较佳值,聚合温度为80度时活性最高,H_2的加入使催化剂的活性明显降低且聚合物的分子量也减小;、新型氯化镁载体负载单活性中心催化剂催化乙烯聚合本文创造性采用固体MAO与无水氯化镁直接进行共研磨,制备出了一种的新型的氯化镁载体,并将该载体用于多种单活性中心催化剂的负载化研究,研究表明: 1、通过BET、XRD的表征,再次证实了MAO与MgCl_2之间具有直接作用,与XPS的研究结果相一致; 2、当研磨时间高于12小时时,延长研磨时间对载体的结构没有显著的影响; 3、将配合物3负载于共研磨时间不同的一系列载体上,乙烯聚合结果表明,尽管共研磨时间对负载催化剂的活性具有明显的影响,但是它对聚合物的性能以及催化剂的影响不明显。

Path analysis of nine traits, which were highly correlated with tree volume, showed that the direct contribution of DBH and height on the variations of tree volume took up about 63% of the total variations. On the other hand, those traits, such as tree crown and wall/lumina, affected the volume strongly by indirect way of the genetic correlation with DBH and height.

通过对与材积性状紧密相关的胸径等9个性状的通径分析发现,胸径和树高两个性状对单木材积的直接作用占材积总变异的63%,其余性状的直接控制作用很小;冠幅、壁腔比等性状通过胸径对单木材积具有很大的间接遗传控制作用,这种控制是通过与胸径、树高的遗传相关实现的。

Results TAEP can significantly decrease the contents of monoamine neurotransmitter in the straite body and the bordering area of rat forebrain, increase the contents of neutral and acidic metabolites in the straite body, and increase the contents of 5-HIAA and HVA of the monoamines in the bordering area of rat forebrain.

结果 TAEP对大鼠纹状体的单胺类神经递质的含量具有显著的降低作用,同时升高纹状体单胺类递质中性及酸性代谢产物的含量;对大鼠前脑边缘区的单胺类递质具有显著的降低作用,同时显著升高边缘区单胺代谢物5—羟吲哚乙酸(5—HIAA),高香草酸的含量。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。