半纤维素酶

The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,300 IU/g of KDN pectinase,35℃、6 h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 500 IU/g of TZ-888 compound enzyme,45 ℃,6 h.

摘 要：本研究根据苎麻胶质的主要成分为果胶、半纤维素和木质素，用高活性的KDN果胶酶和TZ-888复合酶（主要是木聚糖酶和甘露聚糖酶）从pH值、浴比、酶用量、金属离子、温度、脱胶时间等方面进行单因素试验，然后选取影响显著的浴比、酶用量、温度，时间进行四因素三水平的脱胶试验，结果表明鲜麻采用KDN果胶酶脱胶最佳组合为浴比1：18，pH 8.6， 1 mmoL/L Mg2+，酶用量300 IU/g，温度35 ℃、时间6 h时处理，TZ-888复合酶脱胶最佳组为浴比1：12，pH 4.0， 1 mmoL/L Ca2+，酶用量500 IU/g，温度45 ℃，时间6 h进行脱胶为优化的试验条件。

The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on dry ramie degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,20 IU/g of KDN pectinase,4℃、4h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 300 IU/g of TZ-888 compound enzyme,40 ℃,5 h.

摘 要：本研究根据苎麻胶质的主要成分为果胶、半纤维素和木质素，用高活性的KDN果胶酶和TZ-888复合酶（主要是木聚糖酶和甘露聚糖酶）从pH值、浴比、酶用量、金属离子、温度、脱胶时间等方面进行单因素试验，然后选取影响显著的浴比、酶用量、温度，时间进行四因素三水平的脱胶试验，结果表明干苎麻采用KDN果胶酶脱胶最佳组合为浴比1：12，pH 8.6， 1 mmoL/L Mg2+，酶用量200 IU/g，温度45 ℃、时间4 h时处理，TZ-888复合酶脱胶最佳组为浴比1：18，pH 4.0， 1 mmoL/L Ca2+，酶用量300 IU/g，温度40 ℃，时间5h进行脱胶为优化的试验条件。

The degradation of stalk cellulose and hemicellulose are completed by a great variety of enzymes.

秸秆纤维素及半纤维素成分的降解是在多种酶协同作用下完成的。

Virulence test demonstrated that the recombinant AHL-Lactonase has a strong anti-pathogenic activity to Erwinia cawtovora, and the addition of AHL-Lactonase to the medium of pathogen could reduced the activity of pectate lyase, prctin lyase, cellulase and polygalacturonase after 20h.

致病性检测表明，重组AHL-Lactonase对胡萝卜软腐病菌具有较强的抗病活性，在致病菌培养基加入AHL-Lactonase培养20h之后，培养基上清之中果胶酶(pectate lyase and prctin lyase，PL)、纤维素酶(cellulase，Cel)和多聚半乳糖醛酸酶(polygalacturonase，PG)的活性都存在不同程度的减小。

Amylolytic, betagulcanase, hemicellulase,protease,galatosidase.

amylolytic ， betagulcanase ，半纤维素酶，蛋白酶， galatosidase 。

Compared with the control, majority treatments could promote the activity of hemicellulase, and the effect on the activity of pectinase was less than that of other two enzymes.

与对照相比，多数药剂处理后能促进半纤维素酶的活性，同时药剂对果胶酶活性的影响不如对其它两种酶活性的影响明显。

The activity of polygalacturonase,PME,cellulase and the soluble pectin content of MA stor-age were significantly lower than those of control; while the flesh firmness,cellulose content ,protopectin content were sig-nificantly higher than those of control. Results showed that MA storage retarded the softening process of mulberry fruit.

研究了在(2±1)℃下，MA贮藏对桑果细胞壁组分和水解酶活性的影响，结果表明：MA贮藏可抑制桑果呼吸强度和乙烯释放量，MA贮藏桑果的纤维素酶、果胶甲酯酶和多聚半乳糖醛酸酶等细胞壁水解酶活性和水溶性果胶含量显著低于对照，而果实的纤维素和原果胶含量显著高于对照，从而延缓桑果的软化。

All additions rapidly decreased silage pH under different DM contents of M.sativa.M.sativa(DM 38.45%) ensiled with lactic acid bacteria+cellulase gave the best results: ammonia nitrogen content was reduced,and most CP was conserved and most lactic acid produced.

结果表明，不同干物质含量的苜蓿青贮时，乳酸菌+纤维素酶的添加均能使青贮pH值快速下降；苜蓿半干青贮(干物质含量为38.45%)可使青贮氨态氮含量显著降低，并保存有较多的粗蛋白质，生成较多乳酸，其中以添加乳酸菌106 cfu/g+纤维素酶0.05 g/kg的青贮效果最好，其青贮综合评定得分最高，达93分。

The relative contents of celluloses and hemicellulose were decreased from 27.1% to 13.1% and from 20.3% to 11.9% respectively after enzymolysis for 6d.

黄孢原毛平革菌培养12d时LiP酶活达到最大值11.3u/g，15d时Lac酶活达到最大值0.0992u/g，秸秆降解集中在10-20d间，第25d时菌解结束，干基总损失率为18.94%；再经过6d的酶解，纤维素、半纤维素的相对含量分别从27.1%，20.3%下降到13.1%，11.9%。

Results show that maximum activities of LiP and Lac reach 11.3 u/g at the 12th day and 0.0992 u/g at the 15th day, rapidly degraded during 10～20 d, and the total loss rate of dry corn stalk is 18.94% after cultivation for 25d. The relative contents of celluloses and hemicellulose are decreased from 27.1% to 13.1% and from 20.3% to 11.9%, respectively after enzymolysis for 6 d.

黄孢原毛平革菌培养12 d时木素过氧化物酶酶活达到最大值11.3 u/g，15d时漆酶酶活达到最大值0.0992 u/g，秸秆降解集中在10～20 d，第25d时菌解结束，干基总损失率为18.94%；再经过6 d的酶解，纤维素、半纤维素的相对含量分别从27.1%、20.3%下降到13.1%、11.9%。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。