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In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分,我们按照交替氧化酶(Alternative Oxidase,AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列,用固相法合成由12个氨基酸残基组成的多肽,将此多肽与α-牛胰凝乳蛋白酶原A相连,用作半抗原,免疫家兔制备人工合成12肽的抗体。

For developing an immunoassay for multiple residues of pyrethroid pesticides, the hapten N-2- carbanmoyl-(3"-phenoxyphenyl) methyl 3-(2"-Cldoro-3",3",3"-trifluoropropenyl)-2, 2-dimethylcyclopropane- carboxylate was synthesized from cyanohydrin and 3-(2"-Chloro-3",3",3- trifluoropropenyl)-2, 2-dimethylcyclopropanecarboxylic acid via hydrolysis and esterification. The synthesis of the hapten was foundation of preparaing anti- cyhalothrin polyclonal antibodies.

为了制备对单一拟除虫菊酯高特异性的抗体,以菊酸和氰醇为起始原料,通过水解、酯化反应合成了半抗原N-2-氨甲酰-(3-苯氧基苄基)甲基-3-(2-氯-3,3,3-三氟丙烯基)-2,2-二甲基环丙烷羧酸酯,为进一步制备抗三氟氰菊酯的特异性抗体奠定基础。

The improved vaccine provides a prolonged and potent immune response against the peptide or other small hapten.

改进的疫苗可提供对肽或小的半抗原持久和有效的免疫应答。

The vaccine is especially effective for vaccinating a human or animal against a peptide or other small hapten.

该疫苗对接种给人或动物以抵抗肽或其它小的半抗原是尤为有效的。

Using hapten penicillin G as a model, the nanogold in size of 9 nm was used to label rabbit anti-penicillin G antisera to obtain an immunonanogold probe for PG.

以半抗原青霉素G为模型,用粒径为9 nm的金纳米微粒标记羊抗兔青霉素噻唑蛋白抗体制备了青霉素G的免疫纳米金共振散射光谱探针。

UV-vis titration spectra have been applied to obtain the stability constants of complexes and their stoichiometry.

紫外滴定光谱法定量计算出了半抗原GSH-S-DNP与2-diSeCD所形成复合物的结合常数远远大于其与β-CD的结合常数,且结合比都是1:1。

New PlatinumBright nucleic acid labeling kits offer a proprietary ULS labeling molecule bound to a choice of fluorescent dyes or haptens, for use in fluorescence in situ hybridization applications.

新型PlatinumBright核酸标记试剂盒具有知识产权的ULS标记分子,可以连接荧光染料或者半抗原,用于荧光原位杂交实验。

By synthesizing the hapten LBc starting from the metabolite of bifenthrin (2-methyl-3-phenylbenzyl alcohol), LBs (2-methyl[1,1-biphenyl]-3-ylmethyl 3-carboxyl-2,2-dimethylcyclopropane carboxylate and Lby (2-methyl[1,1-biphenyl]-3-ylcarboxylic acid , Three haptens were praparaed which were used in next step.

利用联苯菊酯的代谢物联苯醇合成了联苯菊酯的半抗原LBc(2-甲基-3-苯基苄基氧基羰基丙酸)、LBs(2-甲基-3-苯基苄基-3-羧基一2,2-二甲基环丙烷羧酸)和LBy(2-甲基-3-苯基苯甲酸)。

Results: A hybridoma cell line secreting McAb for specificity to dimethoate was obtained.

结果:通过偶联,获得免疫原和包被原,半抗原与BSA和OVA的偶联比分别为13:1和11:1。

Methods: penicillin, as a kind of hapten, was conjugated with carrier protein to form complete antigen and then was used to immunize balb/c mice. hybridoma cells secrecting mab against penicillin were developed.

将半抗原青霉素和载体蛋白偶联后免疫balb/c小鼠,应用杂交瘤技术建立稳定分泌抗青霉素mab的杂交瘤细胞株。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

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但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。