半小时的

There are many local taxis at the terminal; the price is about 200 RMB for a round-trip. It takes about half an hour to get to the entrance of Jinshanling; they will pick you up at the exit of Simatai. The time is a little longer on the way back to Miyun bus terminal from Simatai.

在终点有很多当地的出租车，价格一般是200元一个来回，把你送到金山岭，大概要半个小时，然后在司马台接你回密云公交车站，时间也差不多半个小时。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, before the tour ends, we will leave the car behind and venture forth on a horse or a camel for one hour and half into the ancient history of Giza plateau, enjoying watching the sunset in the pyramids. And then back to your hotel

塞加拉金字塔的第一个和世界上最古老的石结构，驱动器后，我们的首都孟菲斯的古老王国和最重要的一个城市的整个历史的古埃及，打破吃午饭，然后开车到吉萨访问伟大的吉萨金字塔和神秘的狮身人面像，在巡回赛结束后，我们将离开车后面和合资企业提出的关于马或骆驼的一个半小时到古代历史的吉萨高原，享受观看日落的金字塔。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, before the tour ends, we will leave the car behind and venture forth on a horse or a camel for one hour and half into the ancient history of Giza plateau, enjoying watching the sunset in the pyramids. And then back to your hotel

塞加拉金字塔的第一个和世界上最古老的石结构，驱动器后，我们的首都孟菲斯的古老王国和最重要的一个城市的整个历史的古埃及，打破吃午饭，然后开车到吉萨访问伟大的吉萨金字塔和神秘的狮身人面像，在巡回赛结束后，我们将离开车后面和合资企业提出的关於马或骆驼的一个半小时到古代历史的吉萨高原，享受观看日落的金字塔。

At length he would reach the corn, and selecting a suitable ear, frisk about in the same uncertain trigonometrical way to the topmost stick of my wood-pile, before my window, where he looked me in the face, and there sit for hours, supplying himself with a new ear from time to time, nibbling at first voraciously and throwing the half-naked cobs about; till at length he grew more dainty still and played with his food, tasting only the inside of the kernel, and the ear, which was held balanced over the stick by one paw, slipped from his careless grasp and fell to the ground, when he would look over at it with a ludicrous expression of uncertainty, as if suspecting that it had life, with a mind not made up whether to get it again, or a new one,or be off; now thinking of corn, then listening to hear what was in the wind.

最后，它终于到了玉米旁，拣定一个玉米穗，还是用那不规则三角形的路线跳来跳去，跳到了我窗前堆起的那一堆木料的最高峰上，在那里它从正面看着我，而且一坐就是几个小时，时不时地找来新的玉米穗，起先它贪食着，把半裸的穗轴抛掉；后来它变得更加精灵了，拿了它的食物来玩耍，只吃一粒粒的玉米，而它用一只前掌擎起的玉米穗忽然不小心掉到地上了，它便做出一副不肯定的滑稽的表情来，低头看着玉米穗，好像在怀疑那玉米穗是否是活的，决不定要去拣起来呢，还是该另外去拿一个过来，或者干脆走开；它一忽儿想看玉米穗，一忽儿又听听风里有什么声音。

On Sunday afternoon, the festival's final day, Sugarland was playing a concert at three-thirty. The 2008 queen and court were in the front row, looking untouchably glamorous in the last few hours of their eminence.

星期天下午，草莓节的最后一天，Sugarland在星期天下午的3点半举行了一次音乐会。2008年的草莓皇后和众佳丽们站在前排，在她们的最后几个小时的耀眼的时刻看着那些不可及的魅力。

Ultimatebet.com , I emceed poker tournaments that raised almost $10 million for charity, we donated hundreds of thousands of dollars of our own money to charity, my wife and I raised two fine boys almost into adulthood, I launched a clothing line and a publishing company (Phil's House Publishing), we launched a cell phone game (PHTH Phil Hellmuth's Texas Hold'em) that has 1.3 million users in the USA alone, we just launched another cell phone game on IPHONE and other smart phones in over 180 cell phone systems across the world, I bought a piece of 15 different companies including the amazing online device reputation security company "Iovation" and the industry leading "Card Player Magazine,""Milwaukee's Best" beer here put a picture of me and one of my lines on 12 million beer cans, I am the star of the WSOP video game across all systems, I sold nearly $1 million dollars worth of my four-and-a-half hour long poker course online at philssecrets.com, I sold tons of my "Phil Hellmuth's Million Dollar Secrets" videos, I sold 50,000 copies of my second book "Bad Beats and Lucky Draws," Phil's House Publishing put out its first book "Deal Me In"(a few months ago and sold 15,000 copies already), I put my poker column into over 50 newspapers, I wrote a series of columns for "USA Today"(7 million readers) and "USA Today Online"(7 million readers), in some ways I helped grow the sport of poker, we put together a great team of people to run my life and companies from a COO and a personal assistant to a full time maid and a bill payer, and I wrote a New York Times Best Selling book "Play Poker like the Pros."

我帮助建立了www.Ultimatebet.com网站。我主办的扑克锦标赛筹集了将近100万美元的善款。我自己捐献了数万美元给慈善机构。我的妻子和我一起将两个孩子养育成人。我投资了一个服装生产线和一家出版公司（Phil's House Publishing）。我们推出了一个手机扑克游戏（PHTH Phil Hellmuth's Texas Hold'em），这个游戏在美国就有1千2百万玩家。我们刚刚又在Iphone和超过180个智能手机系统上推出了另一个手机游戏。我入股了15个不同的公司，包括著名的在线设备安全公司&Iovation&和业界领先的&Card Player&杂志。&Milwaukee' Best&啤酒把我的照片和我的服装产品印制到他们一千二百万个啤酒罐上。我是跨平台WSOP视频游戏中的明星角色。我的网站philssecrets.com售出了将近1百万美元的在线扑克教程（4个半小时长）。我卖出了无数&Phil Hellmuth百万美元的秘密&视频教程。我的第二本书&Bad Beats and Lucky Draws&卖出了50，000本。&Phil's House Publishing&出版了它的第一本书&Deal Me In&（几个月来已经卖出了1，5000本）。我的扑克专栏在50家不同的报纸上刊登。我为&USA Today&和在线版&USA Today Online&写一个系列专栏（有700万读者）。在某种程度上，我帮助了扑克运动的普及。我组建了一个优秀的团队来运营我的生活和公司，从全职保姆和账房到公司COO和私人助理。我的书&Play Poker like the pros&是纽约时代周刊上的畅销书。

Grand Chu came after the first task is to cut a half an hour, Minister Wu,黑老大into a cell on the half hour from the beginning that the decline in the rule of cutting edge Minister King will step down, also to hit on the Chu Secretary of State, but later the company asked the journey, not to worry about, it is no longer clear兴师动众minister hit, but called on the Minister Shou so many invaders in situ.

大楚来了之后，第一项任务是半个小时砍一次吴国大臣，黑老大就半个小时进一次牢房，一开始，认为砍大臣下降统治力会使国王下台，就也回砸楚的大臣，但后来问过征途公司，这个顾虑没有了，就不再兴师动众砸楚大臣了，只是号召守大臣，让入侵者多多原地。

Although the center stage was free and open, the strippers had shown everything they were supposed to show. Its not that much fun to watch, only reminded Emily the way she saw naked women in the public changing rooms. Just naked women, except there are noisy half-drunk men with clothes on who were sitting next to them. Emily was disappointed by these strippers, strip clubs is one thing crossed out on her curiosity list.

台上脱的虽是自由开放，台下却看的纳闷，中心台上舞动的女人，好象小时侯在公共浴池看到的女人一样，一件件的脱到一丝不挂，除了旁边多了些有穿着衣服喧嚣的喝的半醉的男人。

B for their personal, property damage and liability will be borne by the economic self-B; 6.B in the service process, the government should abide by the state's environmental laws and regulations.prevent and reduce service to the process of environmental pollution; 7, B car buyer should ensure that the number Exceptionally,B should promptly notify the buyer, the two sides resolved, in accordance with the obligation of the two parties request the deployment of B vehicles.ensure the successful completion of tasks; 9.B subordinated obligation of the two parties will change in accordance with the needs of midnight make off line or location (under the premise of not violating the traffic law).obey buyer; 10, the leasing period, while B causes vehicles encountered administrative punishment.Punishment from B commitments; 11, the leasing period,B or B reasons for the failure of transport provided at B should be restored within half an hour, could not be saved.contractual ties with timely passenger vehicles grades, from B to bear the additional costs.

乙方自身的人身、财产损害及应承担的经济赔偿责任概由乙方自行负责； 6、乙方在服务过程中，应遵守国家环境方面的相关法律法规，避免和减少服务过程中对环境的污染； 7、乙方应保证甲方用车数量，如遇特殊情况，乙方应及时通知甲方，双方协商解决，乙方有义务根据甲方要求另行调派车辆，保证任务顺利完成； 9、乙方有义务服从甲方根据工作需要零时性改变行驶线路或上下客地点（在不违反交通法的前提下），服从甲方安排； 10、租用期间，因乙方原因导致车辆在途中遇行政处罚的，处罚费用由乙方承担； 11、租用期间，因乙方的原因或乙方提供的交通工具出现故障时乙方应在半小时内修复，不能修复的，及时联系合同约定同档次车辆转客，产生的额外费用由乙方承担。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。