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Semiquantity RT-PCR method was used to detect the COLlal and COL3al in the ligamentum teres at mRNA level.

采用半定量RT-PCR及Western-Blot法检测COL1a1、COL3a1在mRNA及蛋白水平的表达。

In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

采用半定量RT-PCR、ABC免疫酶标和流式细胞术以及荧光素/荧光素酶法,研究了七个血源细胞系中CD39的表达和功能。

Methods The MAL gene expression in 45 cancer patients was detected with RT-PCR and semi-quantitative RT-PCR technique.

采用RT-PCR和半定量RT-PCR方法检测45例食管癌组织和癌旁食管黏膜组织中MAL基因的表达。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤细胞OS732, 6d后,进行细胞形态学观察、活细胞计数、流式细胞仪分析细胞周期、半定量RT-PCR检测细胞周期调控蛋白CyclinD(D1、D2、D3)、CDK4、P21 mRNA表达的改变。

It was also found that Cap43 expression in patient with adenocarcinoma was higher than those with squamous cell carcinoma(P.01). Western blot also showed high expression of cap43 in lung cancer. Cap43 expression in mucoid adenocarcinoma was significantly higher than the other pathological types of lung cancer.

免疫印迹也显示,Cap43蛋白在非小细胞肺癌组织中高表达;其半定量结果显示,Cap43蛋白在肺粘液性腺癌组织中的表达量明显高于其它组织学分型肺癌组织(P.01)。

Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.

方法采用半定量PCR方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。

With this method, semiquantitative detection of sulfadiazine can be completed in less than 15min.

该免疫层析试纸可以在15min内完成对磺胺嘧啶残留的半定量检测。

Semiquantitative PCR analysis of mRNA was performed to determine the relative levels of IL-2 message.

采用半定量RT-PCR检测系统中IL-2 mRNA的表达。

Methods The expression levels of TNF-α and IL-1α mRNA by PBMCs were assessed by semiquantitative RT-PCR.

TNF-α和IL-1α mRNA的表达水平采用半定量逆转录-聚合酶链反应进行检测。

The score criterion was worked out as a semiquantitative index to assess imaging qual...

制订了图像质量评分标准作为比较的半定量指标。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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