半定量的

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

To explore the hemogram of healthy adults after the semiquantitative exercise test and to explore its potential significance.

目的观察正常人半定量运动试验后的血象变化，探讨半定量运动试验潜在的临床意义。

To explore the hemogram of healthy adults after the semi?quantitative exercise test and to explore its potential significance.

目的 观察正常人半定量运动试验后的血象变化，探讨半定量运动试验潜在的临床意义。

The distributary river course reservoir in the Lower Member of Minghuazhen Formation in an oilfield of Bohai Basin was modeled. The modeling units were divided using the integrated geological modeling method based on seismic, log and geological data. The sedimentary microfacies features of the modeling units were described. The geological knowledge base, including quantitative description of microfacies in every unit, probability function of facies, connection probability of sand body, was established. The geological model of reservoir was proposed on the basis of stochastic reservoir modeling by using an objectbased simulation method.

以渤海某油田新近系明化镇组下段缓坡三角洲平原亚相分流河道储层为例，利用以精细地质建模为目的的地震、测井和地质一体化综合研究方法，合理划分模拟单元并定量描述各单元内沉积微相特征；结合相概率函数和砂体连通概率等定量约束条件，建立了半定量—定量储层地质知识库，实现了等时建模、成因控制相建模、确定性条件约束随机建模三者的有效结合；采用基于目标的随机模拟方法建立了储层地质模型。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后，用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化；用半定量RT-PCR检测β-catenin mRNA表达的变化；用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况；用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2，cyclinD1， c-fos， c-Jun mRNA表达的情况；用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

METHODS: Spleen cells of 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h in the coculture system were observed and the expression of RNA editase was detected by semiquantitative PCR and was contrasted with the mouse spleen cells in a singleculture system. The mouse model with the immune system restrained by FK506 was made, its spleen cells were cocultured with stimulated cells and then the effect of FK506 on RNA expression of editase was detected by semiquantitative PCR.

观察共培养1，2，4，6，8，10，12，16，20及24 h的小鼠脾细胞，用半定量PCR的方法检测RNA编辑酶表达的变化，并以相同时段单独培养的小鼠脾细胞RNA编辑酶的表达情况进行对照；制备免疫功能受FK506抑制的小鼠模型，取其脾细胞与刺激细胞进行相同时段的共培养，用半定量PCR的方法检测FK506对脾细胞RNA编辑酶的影响。

After semiquantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar timecount curves, so the semi0quantitative exercise test may replace the epinephrine test.

结论半定量运动试验和肾上腺素试验后WBC、NEUT和LYM有相似的时间计数曲线，半定量运动试验有取代肾上腺素试验的可能性。

After semiquantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar timecount curves, so the semiquantitative exercise test may replace the epinephrine test.

半定量运动试验和肾上腺素试验后WBC、NEUT和LYM有相似的时间计数曲线，半定量运动试验有取代肾上腺素试验的可能性。

The approaches of semi-quantitative simulation include Q2 algorithm, Q3 algorithm, self-explanation simulation, fuzzy qualitative simulation, numerical interval simulation and other techniques for the improvement of semi-quantitative simulation.

半定量仿真方法主要包括：Q2、Q3、自解释仿真、模糊定性仿真、数字区间仿真以及几种半定量仿真算法的改进。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。