半定量的

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

Methods Semi -quantitative reverse transeriptase - polymerase chain reaction was used to examine the expression of BCRP gene in 62 cases of malignant hematologic diseases, BCRP/β2 - MG ratio was defined as BCRP expressible level.

方法应用半定量逆转录-聚合酶链反应，β2微球蛋白基因作内参照，以BCRP／β2-MG的比值作为BCRP基因的相对表达水平，检测62例恶性血液病BCRP基因的表达。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

Results of the RT-PCR showed that the increased transcription of T6 is correlated with the infection by plasmodium parasites.

对与先天免疫相关的分子进行的半定量实验表明，大劣按蚊T6基因的转录与感染疟原虫相关。

The deduced amino acid sequences of HbHDT1 showed high identities (65％, 59％, 50%, 45%, 43％ and 40%, respectively)to those of the histone deacetylase from Ricinus communis, Populus trichocarpa, Solanum chacoense, Trifolium pretense, Glycine max and Triticum aestivum.

该氨基酸序列与蓖麻、毛果杨、马铃薯、红花苜蓿、大豆、小麦的HDACs家族成员的同源性分别为65％、59％、50％、45％、43％和40％。半定量RT-PCR分析结果表明HbHDT1在巴西橡胶树的花、愈伤组织、胚、叶、芽、胶乳中均有表达。

SPECT perfusion examination is mostly used to semiquantitatively measure the cerebral blood flow.

目前常用於临床上的SPECT perfusion检查是已经被肯定的半定量测量脑血流的方法。

Mix to kidney spherule respectively the qualitative pathology change degree between kidney canaliculus undertakes half ration gives a mark, immune group is changed detect the expression of Pax2 and WT1, expression of analytic kidney Pax2, WT1 and nephritic pathology integral and its are both the relation between.

分别对肾小球和肾小管间质的病理改变程度进行半定量评分，免疫组化检测Pax2和WT1的表达，分析肾脏Pax2、WT1表达和肾脏病理积分及其两者之间的关系。

METHODS: Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice.

将对数生长期的胃癌细胞株MKN45接种到裸鼠皮下，成瘤后用制备好的分别含GCRG213正、反义克隆和RNAi片段的腺相关病毒分别在肿瘤局部注射，以空病毒和生理盐水对照，继续喂养2 wk后处死，取肿瘤测量重量，并用半定量RT-PCR检测肿瘤组织GCRG213mRNA表达水平。

Methods: Five 10-23 deoxyribozymes (DZ1-DZ5) with 2 phosphorothioate groups at 5' and 3' end were designed and synthesized. Full sequence ET-1 RNA was transcripted and FAM-labeled 10-23 deoxyribozyme was used to select cleavable 10-23 deoxyribozyme in vitro and to detect intracellular uptake. The content of ET-1 mRNA was measured by semi-quantitative RT-PCR after 10-23 deoxyribozyme was transfected into cultured neonatal rat cardiomyocytes.

体外转录ET-1全长RNA底物，设计并合成5条ET-1 10-23脱氧核酶(DZ1~DZ5)，其5'及3'端各有2个核苷酸硫代修饰，体外切割ET-1RNA底物，筛选有效脱氧核酶；5'标记荧光素FAM的10-23脱氧核酶瞬间转染新生大鼠心肌细胞以观察对10-23脱氧核酶的摄取；采用半定量RT-PCR检测ET-1基因的表达。

Analysis of semi-quantitative RT-PCR revealed a distinct reduction of endogenous OsVP1 expression levels in RNAi transgenic lines compared with wild-type plants. Germination experiment showed that the percentage of geminated seeds from T1 transgenic plants was higher than that of wild-type plants.

半定量RT-PCR分析显示，转基因植株胚中的OsVP1表达量明显低于野生型日本晴植株；萌发试验表明，转基因植株T1种子的萌发速率与野生型日本晴相比有明显的提高。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。