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The differential expression profiles of 588 known genes were determined using Atlas human cDNA expression array. Fifteen genes were differentially expressed following the overexpression of BNIPL-1. Most of them were involved in cell proliferation or cell apoptosis. Furthermore, the differential expression result was confirmed by semiquantitative RT-PCR.

然后采用人Atlas cDNA expression array对新基因BNIPL-1在细胞内的下游调控事件进行了初步研究。588个已知基因中有15个的表达发生了改变,其中大部分是细胞生长和凋亡相关基因,半定量RT-PCR验证了杂交结果的可靠性。

The deduced amino acid sequence of red sea bream MHC classⅡB 1(Chma-DAB*0101) had 87.1%,85.1%,87.1%,90.4%,87.1%,90.8% identity with those of red sea bream classⅡB 2, 3, 4, 5, 6, 7(Chma-DAB*0201-Chma-DAB*0701), respectively, and had 75.2%, 74.5%, 55.9%, 55.1%, 34.3% and 30.4 % identity with those of striped sea bass, cichlid, rainbow trout, Atlantic salmon, mouse and human, respectively.

以正常鱼和鳗弧菌感染后真鲷组织为材料,以反转录合成的第一链cDNA为模板,应用RT-PCR半定量检测方法对真鲷的MHCⅡB基因组织表达特点进行了研究,结果表明,在正常真鲷组织中,其中较强的表达于鳃、脾、头肾、肾、胃、小肠和心脏中,在肌肉和血中表达最弱。

A series of p hydroxybenzoic acid oligomers were synthesized of p acetoxybenzoic acid (4 ABA). Since the oligomers are insoluble in most organic solvent, we tried to analyze the degree of polymerization of the oligomers half stoichiometricly by the FT IR method.

用对乙酰氧基苯甲酸合成了一系列对羟基苯甲酸的齐聚物,由于这些齐聚物不溶于大部分有机溶剂,因此尝试用傅立叶红外谱半定量地分析齐聚物的聚合度。

Observe the change cardiac rhythm and rate;The expression of GimRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴,观察针刺前后心律变化;用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Observe the change cardiac rhythm and rate;The expression of Gi-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴,观察针刺前后心律变化;用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Methods: Arrhythmia was induced with cordilox, and acupuncture two-aide "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴,观察针刺前后心律变化;用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白 mRNA的相对含量。

Methods: tachyarrhythmia was induced with aconitine, and acupuncture two-side "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴,观察针刺前后心律变化;用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Besides its high sensitivity and specificity, the semi-quantitative determination by"naked-eye detection"is possible by this method.

该方法用于氟离子的检测不仅具有高的选择性及专一性,且可半定量&裸眼&检测。

Objective To investigate the expression of heat shock protein 60 and analyze the significance in cervical carcinoma.

方法应用半定量逆转录聚合酶链反应和免疫印迹分析法检测子宫颈癌手术切除组织57例,正常子宫颈组织57例,观察热休克蛋白60的表达与子宫颈癌发生发展的关系。

Using above two methods calculate the thermal infrared information in Sitsang to recognize anomaly and receive ideal production.

利用上述两种方法定量的计算西藏地区一年半热辐射值,从热红外卫星遥感数据中识别出热辐射异常,将其与地震资料对比分析研究取得了较为理想的成果。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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