半定量的

Methods Semi-quantitative RT-PCR was employed to analyze the polymerase β mRNA in 41 cases of epithelial ovarian carcinoma, 27 cases of serous cystadenocarcinoma, and 4 cases of mucinous cystadenoma, endometrioid tumor, and 31 cases of normal ovarian tissue.

采用含有内对照的半定量RT-PCR方法，检测41例卵巢癌患者（其中浆液性囊腺癌21例，黏液性囊腺癌13例，子宫内膜样癌7例）及正常卵巢组织31例pol βmRNA的表达。

Methods: 9 cases of exogenic OVC、6 cases of cystoid and infiltrative OVC、15 cases of well-differentiation OSCC and 15 cases of poor-differentiation OSCC were selected; two specimens, which were carcinoma tissues and the sur...

方法取9例外生型口腔疣状癌、6例囊肿／浸润型口腔疣状癌、15例高分化鳞癌、15例低分化鳞癌，应用半定量逆转录多聚酶链式反应方法检测口腔疣状癌和口腔鳞癌中OPN mRNA的表达水平，应用免疫组织化学法检测上述标本中OPN的表达及分布，判断差异。

Methods Semiquantitative RT - PCR was used to detect the expression differentiation of Canstatin among NPC tissues, NPC cells and normal nasopharyn- geal epithelium.

方法利用半定量RT-PCR方法检测Canstatin在鼻咽癌组织、鼻咽癌细胞和正常鼻咽组织中的表达，比较它们之间的表达差异。

The type Ⅰ and type Ⅱ mRNA levels were measured in 15 normal, 45 hyperplastic and 15 cancerous tissues.

为了进一步研究两型5α-还原酶同工酶在正常、良性增生及前列腺癌中的作用，我们应用RT-PCR方法对Ⅰ型及Ⅱ型还原酶mRNA在各种不同前列腺组织中的表达进行了定性及半定量研究。

By using white copper alloy tablet to determine Ni - Cu- Zn X - ray fluorescence instensity qualitively, and doing intensitive normalization may semi - quantitatively analyse major components percent, the results have shown that there is obvious absorption and enhancement effect.

直接用白铜合金片样定性扫描测定Ni、Cu和Zn的X-射线荧光强度值，作强度归一化可以半定量确定主量元素质量百分数，结果发现元素之间有明显的吸收—增强效应。4 Th normalization

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

Methods: The expression of Livin mRNA was measured in 91 AL and 17 chronic myelocytic leukemia patients by semi-quantity reverse transcription polymers chain reaction. 21 healthy adults were also measured as normal control.

采用半定量逆转录-聚合酶链反应检测91例急性白血病和17例慢性粒细胞性白血病患者Livin mRNA的表达水平， 21名健康人的标本作正常对照。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

Methods: The mRNA expressions of VEGF, kinaseinsert domain-containing receptor and fms-like tyrosine kinase1 (flt-1) in the tubal pregnant decidual tissue, the normal oviductal tissue and the early pregnant human endometrial decidual tissue were detected and compared with semiquantitative reverse transcription polymerase chain reaction.

采用半定量RT-PCR方法，检测输卵管妊娠蜕膜组织中的VEGF、KDR、Flt-1 mRNA的表达，并与正常输卵管黏膜及正常宫内早孕子宫蜕膜组织比较。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。