半定量的

Making use of RT-PCR to examine the expression of c-fos and c-myc semiquantitatively which is controlling gene of VSMC.

采用 RT-PCR 法半定量检测 VSMC 的调控基因c-fos 和 c-myc 表达的改变规律。

MDCTA seems to be an effective imaging modality in identifying ISR and can evaluate ISR semiquantitatively.

多层螺旋CT在冠状动脉介入术后冠状动脉支架再狭窄的显示有较高的临床价值，并可作出半定量评估。

RT-PCR was used to detect the expressions of COL1A1 mRNA in 20 patients with idiopathic congenital talipes equinovarus.

采用半定量RT-PCR方法检测20例单纯性马蹄内翻足患儿下肢肌肉及肌腱组织中COL1A1基因mRNA的表达，根据COL1A1基因转录调控区-1 031 bp~+30 bp及第1内含子的序列，设计8对引物， PCR扩增后，采用变性梯度凝胶电泳技术筛查突变并测序。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

We selected 33 segments which show hypokinetic or akinetic at resting echocardiography to undergo identification ofviability.Pulsed-wave Doppler tissue imaging was recorded at mid-point of endocardium of every selected segment, at inner and outer mitral annul us as well.Then we measured the parameters.

应用16节段半定量法分析室壁运动，选取静息状态下低动力或无动力的心肌节段进行存活性的评价，留取受检节段长轴方向心内膜中点及二尖瓣内、外环脉冲组织多普勒频谱图，测量各参数。

Adult male Wistar rats were randomLy allocated to 3 groups:control group, stone-forming group, Alisma Orientalis group. Reverse transcription polymerase chain reaction technique was used to examine bikunin mRNA expression levels in rat renal tissue.

将Wistar大鼠随机分成对照组、结石模型组、泽泻组，采用半定量逆转录聚合酶链反应检测大鼠肾组织bikunin mRNA的表达水平、镜下观察肾组织草酸钙晶体分布，同时检测大鼠血生化、肾钙含量、24h尿钙和尿草酸的分泌量。

According to Q=It, maintain crystal clock to ambulate through observation the accident of time, will explain capacitor takes the size.2 of n mensurably partly.

根据 Q=It，通过观测维持石英钟走动时间的长短，来半定量地说明电容器带电量的大小。2。

The expression of CSE1L gene was identified using these aRNA by sqRT-PCR.

结合RNA保护、显微切割及线性扩增技术，获得纯化鼻咽组织中反义RNA（antisense RNA，aRNA），采用半定量逆转录聚合酶链式反应（semi-quantitative reverse transcriptionpolymerase chain reaction，sqRT-PCR）研究从全基因组表达谱中筛选的候选癌基因-CSE1L在两种不同纯化鼻咽组织aRNA中的表达。

Methods TASMCs were stimulated with LPS in the presence or absence of CCK-8 and/or PKC inhibitor chelerythrine, morphine,-OR mRNA expression was analyzed by RT-PCR 16h after stimulation.

培养大鼠TASMCs，在加入或不加入CCK-8和/或CHE、吗啡的情况下，用LPS刺激细胞16h，用半定量RT-PCR检测κ阿片受体mRNA的表达。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术，对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示，转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃，而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。