半定量的

Methods Turning tests induced by apomorphine and forelimb functional tests were performed. Also, the expression of TH mRNA in mesencephalon of the rats was detected by semi-quantitative analysis of one step RT-PCR.

利用阿朴吗啡诱发旋转试验和前肢功能试验，检测PD大鼠的神经行为学特点，一管法逆转录聚合酶链反应半定量检测其中脑TH mRNA的表达。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene ( COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白(enhanced green fluorescence protein，EGFP)荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原a1链基因(collagen type I alpha chain 1 gene， COL1A1)启动子活性的影响。

Based on the researches mentioned above, choosing 18 typical indices, which on behalf of the natural-human pressure (6), ecological states (8), and responses of the human and society (4), using analytic hierarchy process, the author established an estimate system of regional ecological security, worked out a half quantitative compound index number of regional ecological security in Lazi County.

在以上研究的基础上，选取代表自然和人文压力(6个)、生态系统状态(8)和人文社会响应(4个)的共18个典型指标，利用层次分析法建立区域生态安全状况评价体系，半定量计算出拉孜县区域生态安全综合指数。

Methods: The Hela cells were cultured in vitro and treated with different concentrations(0.125～2.0 mg/ml) of matrine solution for 24～72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology.

选用人宫颈癌Hela细胞进行体外培养，以0.125～2.0 mg/ml的苦参碱分别处理Hela细胞24～72 h后，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡率，透射电镜进行形态学观察，RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。

The HSP70 gene expression was determined in hepatopancreas, spleen, heart and head kidney in green swordtail Xiphophorus helleri challenged by Vibrio alginolyticus 0, 2, 5, 8 and 11 days after administration using RT-PCR. Results showed that tile gene expression of the HSP70 was of tissue specific.

用溶藻弧菌Vibrio alginolyticus感染剑尾鱼Xiphophorus helleri，取感染后第0、2、5、8、11 d的活鱼及感染后第2d濒死鱼，采用半定量RT-PCR方法分别检测HSP70基因在肝胰脏、脾脏、头肾和心脏组织中的表达。

Results The allografts showed a typical symbol of acute rejection with excessive granulocyte infiltration around the vessel wall and myocardial interstice.

采用免疫组化半定量方法检测CTGF在心脏组织中的表达，并分析其与心肌纤维化的相关性。

The EAE rats were induced by injecting subcutaneously an emulsion of GPSCH and CFA. In addition, each rat received an intracutaneous injection of pertussis. Albumin in serum and CSF samples was examined on the days of 4,6,8,10,12,14,16,18 and 20 p.i.. The integrity of BBB was assessed by caculating CSF to serum albumin quotient. At the same time the brains, spinal cords, thymus and inguinal lymph nodes were removed for paraffin sections cutting. These sections were used for HE staining and ICAM-1 semiquantitative immunohistochemical staining. The results showed as follows:1. None of EAE rats on days 4,6 and 8 p.i. showed clinical signs whichbecame apparent on day 10 p.i.(2.33±0.76; n=6). On days 12 and 14 p.i.

检测p.i。第4天、6天、8天、10天、12天、14天、16天、18天、20天血清和CSF中ALB含量，其CSF／S ALB比值作为评价BBB损害的指标；同时段取动物脑、脊髓、胸腺和腹股沟淋巴结制成石蜡切片行HE染色和ICAM-1半定量免疫组化分析，结果显示如下： 1、EAE大鼠p.i.4-8天无任何临床表现；p.i.10天症状开始明显，症状评分为2.33±0.76(n=6)；p.i.12-14天为发病高峰期，症状评分为3.75±0.54(n=12)，病情由最初的活动减少，体重减轻逐渐发展为肢体无力、瘫痪，共济失调，甚至濒死状态；p.i.16天大部分动物症状开始缓解，评分为2.08±0.66(n=6)；p.i.20天疾病症状完全消失。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

应用免疫组织化学和半定量RT-PCR方法，首次发现在大鼠的脑内，包括大脑皮层、室旁组织、下丘脑、中脑、延髓和小脑均有AdM mRNA及AdM的免疫活性物质存在；在人脑内也含有AdM mRNA。

An immunohistochemistry method and image analysis were employed to explore the expression of Caspase3 in the neuroepithelial and mesenchyme cells of each embryo.

用免疫组织化学法和图像分析技术对各组鼠胚的神经上皮和周围间充质细胞凋亡相关蛋白Caspase3的表达进行定性、定位和半定量观察。

PACAP/GHRH mRNA was detected in grouper early embryos but was strongly expressed starting on neurula stage onwards.

在此基础上，利用半定量RT-PCR方法对斜带石斑鱼胚胎及仔鱼期的GHRH／PACAP前体mRNA的表达情况进行了检测。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。