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After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞,0.25%胰蛋白酶消化传代和纯化细胞;通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定:采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

The expression levels of 7 genes (8#, 9#, 13#, 16#, 8A4R19G1, PSC11 and 1O10) are also checked in different organs such as root, term, leaf, flower and gynophores of peanut, using semi-quantitative RT-PCR.

利用半定量RT-PCR技术分析了8#、9#、13#、16#、8A4R19G1、PSC11和1O10在花生不同器官的表达量。

The expression of P2X7 as well as modulation of P2X7 mRNA expression by various inducers in human normal PBMNC was measured by RT-PCR and Immunohistochemical method.

P2X7受体的诱导表达:应用半定量RT-PCR,证明了P2X7在正常人外周血细胞中表达。

The expression of IGF-IR in the difference stages (proliferative stage and involuting stage) of hemangiomas was measured using SP immunohistochemical method and RT-PCR, comparing with vascular malformation and normal skin tissues at same time.

采用免疫组化SP法和半定量RT-PCR法检测不同分期血管瘤中IGF-IR的表达水平,并与血管畸形及正常皮肤标本进行比较。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者,另取癌旁正常乳腺组织作为对照,分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

After semi?quantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar time?

半定量运动试验和肾上腺素试验后WBC、NEUT和LYM有相似的时间?

BaPAL gene sequence was submitted to NCBI under the accession number FJ849059. Semi-quantitative RT-PCR was applied using roots, stems, leaves, flower buds, open flowers and siliquas cDNA as PCR templates, respectively.

半定量RT-PCR结果表明,BaPAL基因在茎、叶、花蕾、开放花朵和嫩角果中均有表达,而且以开放花朵的表达量最大,根中未能检测到。

After 1,4, 7, 10, 14 days of exposure, eight rats of each group were sacrified to assess lung histopathologic changes.

两组分别于暴露满1、4、7、10和14d时,每组取动物8只,行肺组织病理学检查,半定量逆转录聚合酶链反应测定COXI、COXII和COXIIImRNA的表达。

After diluting to a definite volume, aliquots of the extracts are taken, and determinations are made by extraction of the molybdenum-thiocyanate complex with ether in the presence of stannous chloride and then by visua...

苏联及北欧国家的地鬓学者过去在地球化学探矿工作中普通使用光帮半定量方法。美国、英国和加拿大则主要使用化学方法此

The relationships between bone marrow semi-quantitative scores and clinical and haematological variables were investigated.

结果:不同临床分期患者的图像表现形式和半定量计分均有显著差异。

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