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半乳糖酶

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We further expressed the β-galactosidase reporter gene in R. ruber driven by promoter PlacZ. Results indicated that lacZ was an appropriate reporter gene for genetic or metabolic engineering research of Rhodococcus.

采用PlacZ启动子在红球菌宿主中启动b-半乳糖苷酶报告基因,结果表明, lacZ能够在红球菌中高效表达,是优选的红球菌报告基因。

He sputa from 69 suspected lung cancer patients were tested for T antigen using the enzyme galac-tose oxidase.

本研究采用半乳糖氧化酶方法检测69例可疑肺癌患者痰液中的T抗原。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型,同时以何首乌饮灌胃作为延缓衰老组;造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况;测定各组Fas和FasL的信使核糖核酸水平;SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

Transcriptionally active genes have ___ level of DNA methylation when compared to methylation of inactive genes.

C IPTG 与 lacI 基因的产物结合,抑制它的活性E IPTG 作为β-半乳糖苷酶的别构效应物,直接刺激它的活性

Methods With the model of aging mice induced by D-gal, the learning memory ability of mice was measured by using Y-maze, the contents of SOD and MDA in the cerebrums were determined by xanthine oxidation or TBA colorimetry ,the variations of above indices of mice after treatment with NGD were observed.

建立 D-半乳糖致小鼠衰老模型,以 Y型电迷宫检测其学习记忆能力,分别采用黄嘌呤氧化酶法、TBA比色法测定大脑 SOD活性和 MDA含量,观察小鼠服用神经生长液后上述指标的变化。

Maybe it is a new member of b-galactosidase family. bin2a mrna is upregulated by androgen and also epididymis-specific, majorly in caput epididymides.

所以,bin2a可能是b-半乳糖苷酶家族的新成员。bin2a表达受雄激素上调,其表达的组织专一性也很高,只在大鼠附睾有表达,并主要在附睾头部。

Methods In a series of cultured RPE cells, a standard beta-galactosidase histochemistry technique and MTT assay were performed to detect the activity of beta-galactosidase on a varying number (5, 10, 20) of population doublings and age (26, 44, 57) of the donator.

在培养的RPE细胞中,以传代次数(5、10、20)和取材供体的年龄(26、44、57岁)分组,进行标准化的β-半乳糖苷酶活性和细胞增殖能力检测。

The expression of p16INK4a protein was tested by immunochemical assay, and the life span in vitro of nasopharyngeal epithelial cells was calculated as population doublings.

我们应用老化相关半乳糖苷酶染色检测SA-β-Gal活性的表达情况,并利用免疫组化测定p16INK4a蛋白的表达。

Hours after galactose induction , The top expression of recombinant fusion protein has fullfiled, xylanase activity was 62.5IU/mL.

半乳糖诱导24h时重组蛋白的表达量最大,酶活可达62.5IU/mL。

Bp cDNA encoding L-galactono-1,4-lactone dehydrogenase fragment was cloned from fruit of Rosa. roxburghii Tratt by the method of RT-PCR on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc.

根据GenBank中登录的拟南芥、花椰菜、甘薯和草莓等植物的半乳糖内酯脱氢酶基因保守序列设计引物,采用RT-PCR法,从刺梨果实中扩增出该基因的cDNA片段855bp并测序。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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