半乳糖酶

Keywords Fabry disease; angiokeratoma;аgalactosidase

Fabry病；血管角质瘤；а半乳糖苷酶

Objective To Probe the effect of taurine on the quantity of Ca2+ and the activity of Ca2+-ATPase in galactose cataract mice's aqueous humor and lens.

目的 探讨牛磺酸对半乳糖性白内障房水和晶状体中Ca2+含量及晶状体Ca2+-ATP酶活性的影响。

Four positive clones were obtained by the selection of autotrophic phenotype and β-galactosidase assay.

利用营养缺陷型和β—半乳糖苷酶活性筛选到4个阳性克隆，测序结果表明它们具有相同的序列。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

RESULTS: Stable telomerase activity could be detected in the transfected fibroblasts and no signs of cell senescence were found in the fibroblasts cultured for more than 50 doublings.

结果 转染 h TERT的人成纤维细胞能稳定表达端粒酶活性，培养超过 5 0代后细胞仍保持β-半乳糖苷酶阴性。

The effects of pre-picking exogenous hormones treatments, ambient temperature treatments and postripening treatments on the changes of flavone percentages in Ginkgo exocarps during postripeness were studied. The relationship between the changes of flavone percentages and respiration intensity, cell permeability, pectin methy-lesterase, polygala-cturonase activities were also discussed.

该文研究了不同采前激素处理、不同后熟温度条件以及不同的堆沤与水沤方法对银杏外种皮采后后熟过程黄酮含量变化的影响，并对黄酮含量与后熟过程呼吸强度、多聚半乳糖醛酸酶活性、果胶甲酯酶活性、细胞膜透性变化的相关性进行了探讨。

Recent research to α-galactosidase indicates that the enzyme has widely application prospect in fields of feedstuff、 refining sugar、 foodstuff and pharmaceutical industry, et al.

现今对α-半乳糖昔酶的研究表明，该酶在饲料、制糖、食品、医药工业等领域具有广泛的应用前景。

Xanthus. In addition,β-galactosidase activity of ZC16-18 was detected by X-gal assay and the blue color developed was used to mark the colony growth. Time of colour showed that MXAN1334 gene was expressed in the early stage in M.

通过X-gal检测突变株ZC16-18中β-半乳糖苷酶酶活，实验结果显示，含有X-gal的平板上培养的ZC16-18菌落呈现蓝色，表明MXAN1334基因能够表达；颜色呈现的时间分析结果显示，MXAN1334基因表达时间较早。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷