半乳糖酶

Results: VTTEK ANI, the anaerobic bacilli detecting card, showed that Bartonella strains were negative to oxidase, catalase, PO4, PHC,βGAL, aGAL,βGLU, aGLU, URE, GLU, and positive to Amino acid but PRO. The Rapid TD32 A system revealed that all strains reacted negatively to URE,αGAL,βGAL,βGP,αGLU,βGLU, RAF, MNE.

结果：VITEK ANI厌氧菌鉴定卡检测结果：氧化酶、过氧化氢酶、半乳糖苷酶、葡萄糖苷酶等反应阴性，能够分解多种氨基酸，但不能分解脯氨酸，不能利用尿素、葡萄糖等糖类。

Urinary microalbumin was determined with immunoturbidimetry, urinary NacetylD-glucosaminidase,βDgalactosidase（GAL and glycyl proline dipeptidyl aminopeptidase were determined with rate, urinary creatinine was determined with Jaffe rate.

对127例糖尿病患者，采用免疫透射比浊法检测尿mA，速率法检测N乙酰βD氨基葡萄糖苷酶、βD半乳糖苷酶、甘氨酰脯氨酸二肽氨基肽酶，Jaffe速率法测尿肌酐。

Galactosyltransferase 1 is the first cloned glycosyltransferase. It had been regarded as a house-keeping gene for a long time. In our previous investigation, it was found that GaT I was an intraction protein of SGT, and EIAF, one of the Ets family members could regulat its expression.

1，4半乳糖基转移酶是一个最早克隆的糖基转移酶，长期以来被认为是一个管家基因，本实验室以前的工作表明GalT Ⅰ是SGT的一个相互作用蛋白，Ets转录因子家族的成员EIAF可调控其基因的转录。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法，以不同浓度亚硒酸钠及半乳糖作用于晶状体，诱发大鼠晶状体形成白内障，在培养初期测定晶状体内非蛋白质巯基（nonprotein sulfhydryl， NP-SH、蛋白质巯基（protein sulfhydryl ，P-SH）和不溶性蛋白质二硫键的含量，脂类过氧化水平，以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase， GSH-PX)、谷胱甘肽还原酶(GSH reductase， GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase， GSH-S)的活性。

Virulence test demonstrated that the recombinant AHL-Lactonase has a strong anti-pathogenic activity to Erwinia cawtovora, and the addition of AHL-Lactonase to the medium of pathogen could reduced the activity of pectate lyase, prctin lyase, cellulase and polygalacturonase after 20h.

致病性检测表明，重组AHL-Lactonase对胡萝卜软腐病菌具有较强的抗病活性，在致病菌培养基加入AHL-Lactonase培养20h之后，培养基上清之中果胶酶(pectate lyase and prctin lyase，PL)、纤维素酶(cellulase，Cel)和多聚半乳糖醛酸酶(polygalacturonase，PG)的活性都存在不同程度的减小。

LacZ gene was fused with the promoter of the acoR gene and bkdR gene, and two recombined genes were expressed in sigL mutant strain and HD-73 wild strain sequentially.

分别将调控aco操纵子（编码3-羟基丁酮脱氢酶系统）的转录调节基因acoR和调控bkd操纵子（编码催化支链脂肪酸合成的酶系统）的转录调节基因bkdR的启动子与lacZ基因融合，并转入出发菌株和sigL突变体中，测定b-半乳糖苷酶的活性。

Both automatic microorganism detection system and Rapid ID32 A system can read specific biological characteristic of Bartonella strains.

Rapid ID32 A系统检测结果：所有菌株脲素酶、半乳糖苷酶、葡萄糖苷酶阴性，不发酵甘露糖、棉籽糖。

METHODS: D-galactose (1 000 mg·k-1·d-1 ) was injected into mice hypodermically for 8 weeks to induce aging animal model, and Vit-E (100 mg·kg-1; 250 mg·kg-1) was administered for 6 weeks by ig at the 3rd week of making model. After Vit-E treatment for 8 weeks, water maze test was used to determine the ability of mice's learning and memory. The activities of glutathione peroxidase and succinate dehydrogenase, the content of nitric oxide and activity of nitric oxide synthase in the brain tissue were detected separately.

小鼠连续皮下注射D-半乳糖（1 000 mg·k-1·d-1）8周制备衰老模型，并于第3周开始给予维生素E（100 mg· kg-1；250 mg· kg-1）处理；8周后采用水迷宫测定小鼠学习记忆能力，并取脑组织测定谷胱甘肽过氧化物酶和琥珀酸脱氢酶活性，测定一氧化氮含量和一氧化氮合酶活性。

Enzyme that cleaves lactose into galactose and glucose.

将乳糖分解为半乳糖和葡萄糖的酶。

Objective To determine the distribution and influence of lysosomal neuraminidase, protective protein/cathepsin A and β-galactosidase in the inner ear of the mouse, and to observe their auditory alterations in enzyme deficiency.

目的：研究溶酶体神经氨酸酶(neuraminidase， Neul)、保护蛋白／组织蛋白酶A(protective protein/cathepsin A， PPCA)和β-半乳糖苷酶(β-galactosidase，β-gal)在小鼠内耳的分布及相互影响，观察各自酶缺乏时的听功能改变。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷