半乳糖苷

The expression of PAI-1 and β-Galactosidase activity are markers of senescence in various cell types.

表达排- 1和β-半乳糖苷酶的活性，是标志衰老的各种细胞类型。

Liaoning Medical University, Jinzhou 121001 China）Abstract:Objective To study the distribution and liver targeting of glycyrrhetinic acid derivatives (stearin glycyrrhetinic acid ester-3-O-galactosidase, Gal-GAOSt) modifying norcantharidin liposomes (Gal-GAOStNC-LP) in rats.

目的 研究甘草次酸衍生物（甘草次酸硬脂醇酯-3-O-半乳糖苷，Gal-GAOSt）修饰去甲斑蝥素脂质体(Gal-GAOStNC-LP)在小鼠体内的分布及肝靶向性。方法利用薄膜分散法制备甘草次酸衍生物修饰去甲斑蝥素脂质体后，经小鼠尾静脉给药，采用HPLC法检测去甲斑

LacZ gene was fused with the promoter of the acoR gene and bkdR gene, and two recombined genes were expressed in sigL mutant strain and HD-73 wild strain sequentially.

分别将调控aco操纵子（编码3-羟基丁酮脱氢酶系统）的转录调节基因acoR和调控bkd操纵子（编码催化支链脂肪酸合成的酶系统）的转录调节基因bkdR的启动子与lacZ基因融合，并转入出发菌株和sigL突变体中，测定b-半乳糖苷酶的活性。

An alternative method has been proposed in which P agar (contains peptone, sodium chloride, yeast extract, and glucose) supplemented with acriflavin and the β-galactosidase test is used.

登录后回答可以获得积分奖励，并可以查看和管理所有的回答。登录|另一种方法已经提出了在P琼脂（含蛋白胨，氯化钠，酵母提取物，和葡萄糖）辅以acriflavin和β-半乳糖苷测试使用。

Methods: cell biological assay, histochemical staining of senescence associated-β-galactosidase and immunohistochemical staining of proliferating cell nuclear antigen wereused. Results: chondrocytes exhibited various types of amitotic figures.

应用细胞生物学方法、衰老相关-β-半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法，研究正常成人和正常成体大鼠气管软骨组织动力学。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

IPTG was used to induce expression in BL21, and some enzymic indexes of expressed proteins were detected.

用异丙基-β-D-硫代半乳糖苷诱导表达菌并测定表达蛋白的酶学参数。

The technics of producing membrance for glucose and sucrose detecting were optimized. The best condition is: adding fragmentation liquid of the yeast which is breaked up in 450 times for three times, betaking mix enzyme which is prepared with 0.0036g glucose oxidase and 0.5ml kluyveromyces lactis β-galactosidase and 1.5ml distilled water, immobilizing mix enzyme through 2% glutaric dialdehyde for 1 hour.

优化了葡萄糖和蔗糖酶膜制作的工艺条件，结果表明，在以下条件：3层破碎液次数450次的破碎液，浓度配比为0.0036g葡萄糖氧化酶和0.5ml半乳糖苷酶用1.5ml蒸馏水溶解的混合酶液，固定混合酶的戊二醛浓度为2%，时间为1小时，酶的固定效果最为理想。

Objective To observe the replicative senescence-related beta-galactosidase activity in cultured human retinal pigment epithelium cells.

目的观察培养人视网膜色素上皮细胞中复制衰老相关的β半乳糖苷酶活性。

We further expressed the β-galactosidase reporter gene in R. ruber driven by promoter PlacZ. Results indicated that lacZ was an appropriate reporter gene for genetic or metabolic engineering research of Rhodococcus.

采用PlacZ启动子在红球菌宿主中启动b-半乳糖苷酶报告基因，结果表明， lacZ能够在红球菌中高效表达，是优选的红球菌报告基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。