半乳糖苷

Keywords Fabry disease; angiokeratoma;аgalactosidase

Fabry病；血管角质瘤；а半乳糖苷酶

Four positive clones were obtained by the selection of autotrophic phenotype and β-galactosidase assay.

利用营养缺陷型和β—半乳糖苷酶活性筛选到4个阳性克隆，测序结果表明它们具有相同的序列。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Urinary microalbumin was determined with immunoturbidimetry, urinary NacetylD-glucosaminidase,βDgalactosidase（GAL and glycyl proline dipeptidyl aminopeptidase were determined with rate, urinary creatinine was determined with Jaffe rate.

对127例糖尿病患者，采用免疫透射比浊法检测尿mA，速率法检测N乙酰βD氨基葡萄糖苷酶、βD半乳糖苷酶、甘氨酰脯氨酸二肽氨基肽酶，Jaffe速率法测尿肌酐。

Semiquantitative PCR analysis revealed that the levels of pectate lylase and β-galactosidase gene (MA-gal1) transcript were related with the banana fruit ripening and softening.

通过半定量PCR分析表明，所克隆的β-半乳糖苷酶基因（MA-gal1）和果胶裂解酶基因的表达与果实成熟软化相关。

This method is highly sensitive and highly selective and was can be applied to the rutin、quercitrin、quercitrin-3-O-giucoside and quercitrin-3-O-galactose determination in yacon leaves with satisfactory result and also can be used to determine these flavonoids in Chinese herbal medicine and Chinese herbal and crude drugs preparations containing flavonoids.

该方法也可用于含芦丁型黄酮中草药及其制剂中芦丁、槲皮素、槲皮素-3-O-葡萄糖苷和槲皮素-3-O-半乳糖苷的检测。

On a ZORBAX Stable Bound (2.150 mm, 1.8 m), the rutin、quercitrin、quercitrin-3-O-giucoside and quercitrin-3-O-galactose could be separated completely with 0.05 mol/L potassium dihydrogen phosphate and methanol as mobile phase (40:60,V/V) at a flow rate of 0.3 ml/min and detected with a photodiode array detector .

在色谱柱为ZORBAX Stable Bound (2.150 mm， 1.8 m)，流动相为0.05 mol/L的磷酸二氢钾缓冲溶液-甲醇(40：60，V/V)，流速为0.3 ml/min，在检测波长为360 nm的色谱条件下，芦丁、槲皮素、槲皮素-3-O-葡萄糖苷和槲皮素-3-O-半乳糖苷分离效果好，方法灵敏度高，重现性好，用于测定雪莲果叶中的上述几种黄酮芦丁型黄酮，效果满意。

The result demonstrated that mannitol was the best protective substance, followed by raffinose,trehalose and melibiose, L-cysteine, xanthan gum and glycerol showed slightly protective ability.

结果表明，甘露醇对α-半乳糖苷酶的保护效果最好，其次是棉籽糖、海藻糖和蜜二糖；L-半胱氨酸、黄原胶和甘油也起到了较好的保护作用。

Both automatic microorganism detection system and Rapid ID32 A system can read specific biological characteristic of Bartonella strains.

Rapid ID32 A系统检测结果：所有菌株脲素酶、半乳糖苷酶、葡萄糖苷酶阴性，不发酵甘露糖、棉籽糖。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。