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半乳糖

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The cells were transfected with the eukaryotic expression vector αS1-LA-psv and the activity of β-galactosidase was detected after transfected from 24 h to 120 h. Human alpha lactalbumin was detected at 72 h after transfected too.

将构建的真核表达载体αS1-LA-psv 转染奶牛乳腺上皮细胞,培养24~120 h均检测到了β-半乳糖苷酶的表达;培养72 h检测到细胞中人α-乳白蛋白的表达,表达量约为

The production of human alpha lactalbumin was 0.64 g/L approximately. The results indicate that the bovine mammary epithelial cell line have the ability of expressing exogenous gene, the 5′ flanking sequence of bovine αS1 casein gene has the effect of regulating gene expression specifically and the vector αS1-LA-psv which was constructed can coexpress alpha lactalbumin and β-galactosidase.

实验结果表明,建立的奶牛乳腺上皮细胞系具有外源基因表达活性,得到的牛αS1-酪蛋白5′调控序列能作为启动子指导外源基因的表达,构建的真核表达载体能在体外培养的牛乳腺上皮细胞中同时表达人α-乳白蛋白和β-半乳糖苷酶。

Cyandine-3-galactoside was an usual anthocyanine, but cyandine-3-glucoside didnt exsit in Bogbilberry and Lingonberry, the fruit of Lingonberry contained one of four kinds of anthocyanine, which was cyandine-3-galactoside.

其中矢车菊色素-3-半乳糖苷是普遍存在的一种花色苷,而花色苷矢车菊色素-3-葡萄糖不存在于笃斯越橘和红豆越吉林农业大学硕士学位论文越橘果实中花色昔的提取分离、定量和结构鉴定研究橘果实中,红豆越橘果实中只含四种花色普的一种。

Objective: To investigate association between genetic variants of 804 C/A in lymphotoxin-α and 3279C/T in LGALS2 and susceptibility risk of coronary arteriosclerosis disease.

目的:研究淋巴毒素-α804C/A及其配体半乳糖凝集素-2(LGALS2)C3279T基因变异与冠心病罹患风险之间的关系。

The pathogen grew well on the media using glucose, sucrose, fructose, maltose, rhamnose or soluble starch as carbon sources. Galactose, xylose, mannite or sorbose was not beneficial for the growth of the pathogen, however. The media with only organic or inorganic nitrogen sources were not suitable for the pathogen growth although organic nitrogen sources were better than inorganic nitrogen sources. The pathogen could also infect Allium cepa L., A. fistulosum L. and A. odorum L. under artificial inoculation conditions.

在碳源中,以对葡萄糖、蔗糖、果糖、麦芽糖、鼠李糖和淀粉的利用为佳,而半乳糖、山梨糖、甘露醇、木糖和菊糖不利病菌的生长;有机氮源优于无机氮源,但单独应用有机或无机氮作为营养均不适于此病菌的生长;在人工接种条件下,病菌可侵染葱、洋葱和韭等葱属植物。

The main results were as follows. The main nutrition bioactive components in Opuntia Milpa Alta were analyzed using chemical and instrumental analytical methods. The results showed that the content of crude protein and fat was 0.65%~0.68% and 0.30%~0.31% respectively. The total content of the amino acids was 6.81%~7.38%, and Glu was 1.08%~1.52%.The contents of pectin and cellulose was 1.47%~1.68%, 0.87%~1.22% respectively. They were oxalate free and rich in Ca 5.6%, Fe 8.06mg/100g, Zn 3.30mg/100g, VB6 5.62mg/100g, low in tannin 0.02%~0.04%.The bioactive matter content such as polysaccharide, polyphenol, and flavone were high, about 1.27%, 63.14mg/100g, 36.21mg/100g respectively.

主要研究内容如下:采用化学和仪器分析方法,对米邦塔仙人掌中的主要营养和生理活性成分进行分析,结果表明,粗蛋白含量为0.65%~0.68%,脂肪含量为0.30%~0.31%;氨基酸总量为6.81%~7.38%,其中谷氨酸含量达1.08%~1.52%;果胶、膳食纤维分别为1.47%~1.68%和0.87%~1.22%;干样钙含量5.6%,铁8.06mg/100g,锌3.30mg/100g;B族维生素丰富,其中干样VB6含量5.62mg/100g;单宁0.02%~0.04%;不含草酸;生理活性物质如粗多糖、多酚、黄酮类物质分别约为1.27%、63.14mg/100g、36.21mg/100g,半乳糖约占多糖中单糖总量的80%;HPLC检测出8种酚类物质,绿原酸含量最多达42.40μg/g,其次是芦丁和槲皮素,其含量分别为25.02μg/g和10.91μg/g。

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能,分别为:UDP-N-乙酰葡萄糖-2-异构酶基因,吡喃型UDP-半乳糖变位酶基因,糖基转移酶基因(orf3、orf5、orf6和orf8),O-抗原转运酶基因和O-抗原聚合酶基因。

Part Two Experimental Study Objective: To explore the effects of Nei Zhang Wan on experimental SC in rats.

第二部分实验研究目的:观察中药内障丸对D-半乳糖性白内障的防治作用。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法,以不同浓度亚硒酸钠及半乳糖作用于晶状体,诱发大鼠晶状体形成白内障,在培养初期测定晶状体内非蛋白质巯基(nonprotein sulfhydryl, NP-SH、蛋白质巯基(protein sulfhydryl ,P-SH)和不溶性蛋白质二硫键的含量,脂类过氧化水平,以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase, GSH-PX)、谷胱甘肽还原酶(GSH reductase, GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase, GSH-S)的活性。

Objective To study the effect of astaxanthin osseous changes in D-galactose-induced rats.

目的 研究虾青素对D-半乳糖致衰老大鼠骨质的影响。

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