升汞

Result］ The explants were treated by 0.1% HgCl2 for 20 min and the asepsis material could be obtained.

结果］用0.1%升汞处理外植体20 min，可获得无菌材料。

When mercuric chloride was used to sterilize, the optimum sterilization time for stem tips, stem segments and tender leaves were 9, 12 and 7 min respectively.

用升汞消毒，茎尖、茎段和幼嫩叶片的最佳消毒时间分别为9、12和7min。

The multiplication coefficient of the different parts of squama showed gradual downtrend with the increasing of the sterilizing time by the mercuric chloride.

随着升汞溶液消毒时间的增加，各部位鳞片的增殖系数呈逐渐降低的趋势。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

The explant are rhizome and leaf of Pyrola calliantha It has the best result by using 0.1% HgCl_2 for 7min for the explant of rhizome, for 5min for the explant of leaf.

对外植体的消毒方法已经掌握，用0.1%的升汞对鹿蹄草茎段的灭菌采用7min最佳，用0.1%的升汞对鹿蹄草叶片的灭菌采用5min最佳。

Were used as explants to discuss the effect of growth regulators on inducement, multiplication , rootage and callus; and analysed the range of effective factor.

实验结果如下： 1 采用0.10％升汞为消毒剂对外植体进行处理时，茎尖的适宜消毒时间在8 min左右，叶柄为12 min，叶片4 min。

The best explant in the culture was brown leaf,and the explant sterilized in 0.1% aqueous mercuric chloride for 5min has the best effect;At the initial stage in induction,3% glucosan have the best effect following with 1% glucosan+2%sucrose are optimum in the process of culturing;the optimum culture medium is 1/2MS; the preferable medium of callus is 1/2MS+ KT(0.1mg/L)+6-BA(0.05mg/L)+2.4-D(0.4mg/l);differentiation1/2MS+6-BA(0.3mg/l)+NAA0.2+KT(0.05mg/l);and the preferable medium of rooting is :1/2MS+NAA(0.05mg/L).

安祖花组织培养的最佳外植体为棕色叶片；最佳消毒时间为0.1%的升汞消毒5min；最佳碳源：诱导初期3%的葡萄糖的诱导效果最佳，后期培养以2%蔗糖+1%葡萄糖的培养效果较好；最佳基本培养基为1/2MS。

The results showed that pre-treatment and antibiotic marinating for 5 h and suffocating for 12 min as well as ordinary disinfection for 12 min for surface disinfection could effectively control explant contamination. the contaminate rate with this method would be less than 20%.

结果表明：采用抗菌素浸泡5 h、熏蒸12 min并配合常规消毒处理(75%酒精浸泡1 min；3.0%～5.0%的次氯酸钠和0.1%升汞1∶1混合溶液浸泡10～12 min)对外植体进行综合灭菌，能较快地杀灭竹芋外植体表面细菌，使初次接种污染率控制在20%以下。

The tissue culture and rapid propagation of Mozzie buster were studied by using tips and leaves as explants.The results showed that: The optimum disinfect time for the explants with Hgcl2 was 5~7 min.

以蚊净香草的叶片和茎段作为外植体，用0.1%的升汞作为消毒剂，通过试验拟找出最佳的消毒时间及适于外植体的诱导、增殖和生根的培养基。

MERCURY STOCK SOLUTION : Transfer 135.4 mg of mercuric chloride to a 100-mL volumetric flask, and dilute with 1 N sulfuric acid to volume.

MERCURY储备溶液：升汞调动135.4毫克到100ml容量瓶， and稀释与1 N硫酸对容量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。