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In the present paper,the use of 8-hydroxyquinolinecomplexs in precipitate flotation to separate and preconcentrate Cu and Mn,using SDBS as collector,followed by AAS spectrophotometric determination is proposed.

提出了以8-羟基喹啉为沉淀剂,十二烷基苯磺酸钠为浮选剂,沉淀浮选预分离富集与火焰原子吸收光谱法联用测定痕量Cu和Mn的新方法。

The mechanism was probabl y that SDS could form an opposite electro static field when HVEF is on solution of SDS and catalase, and the electronic fi eld strength reduced.

标 签 十二烷基硫酸钠高压静电场过氧化氢酶 SDS high voltage electrostatic field catalase

Objective Using sodium N-lauroyl sarcosine as new decellularization reagent to prepare decellularized matrix for tissue engineering purpose.

目的尝试用十二烷基肌氨酸钠作为脱细胞试剂,制备脱细胞组织工程支架材料,并分析其生物学性能。

Laboratory of Gellular and Molecular Biology, Shaaghai Medical College, Fudan University, Shanghai 200032, ChinaAbstract:Objective Using sodium N-lauroyl sarcosine as new decellularization reagent to prepare decellularized matrix for tissue engineering purpose.

吴春根 王松梅高红阳潘銮凤复旦大学上海医学院细胞与分子生物学实验室,200032摘要:目的尝试用十二烷基肌氨酸钠作为脱细胞试剂,制备脱细胞组织工程支架材料,并分析其生物学性能。

The inclusion bodies were washed with Deoxycholic acid Sodium Salt for several times, and then dissolved in N-Lauroyl Sarcosine Sodium for denaturant.

以脱氧胆酸钠洗涤包涵体,然后用N—十二烷基肌氨酸钠溶解,将溶解包涵体进行透析复性。

Coli. The outer membrane protein were extracted from 38 strains of avian E. coli which were isolated from the dead chickens of chicken breeding farms in 3 areas of Baoding, Qiuhuangdao and Beijing by using ultrasonic cleaving and N-Lauroyl Sarcosine Sodium, and OMP typing was done by SDS-PAGE.

方法]对分离自保定、秦皇岛和北京3个地区规模化养鸡场的病死鸡体内的38株大肠杆菌,采用超声波裂解和N-十二烷基肌胺酸钠进行外膜蛋白提取,利用SDS-PAGE进行Omp分型。

The lowest surface tension of N_xB solution was better at 25℃, and lowest surface tension would down with carbon . cmc( critical micelle concentration) and γ_ in N_(12)B are 4.1 × 10~(-4)mol/L, 24.5 mN.m~(-1),what was smaller than LAS . The cmc of N_(12)B- NaCl are down with NaCl up.The surface tension and cmc of N_(12)B and N_(16)B are higher than that of N_(12)OB and N_(16)OB because of hydrophilic group ,but that of N_(12)B is lower than that of N_(16)B because of hydrophobic group .5. The borate structure and semipolar bond were studied in different pH by infrared spectrum .It indicated that N_xB and N_xOB have not semipolar bond at pH

4、25℃,N_xB系列都有较低的表面张力,并随碳原子数的增加而最低表面张力下降。N_(12)B比同碳原子数的十二烷基苯磺酸钠的最低表面张力34.5mN/m低,且临界胶束浓度为4.1×10~(-5)mol/L,也比LAS低,且随着NaCl浓度的增加而略有下降,比较N_(12)B和N_(16)B,由于C_(16)疏水基的异构体使N_(12)B的临界胶束浓度和临界胶束浓度时的表面张力γ_(cmc都低,由于增加了亲水基团使得N_(12)OB和N_(16)OB的表面张力和临界胶束浓度都要高于N_(12)B和N_(16)B。

Keywords sodium dodecy/benzene sulfonate; hepatic lesion; reverse change of seroenzyme

十二烷基苯磺酸钠;肝损害;血清酶逆向变化

Copolymerization by micellar polymerization [8] is the most usual method in which a surfactant such as sodium dodecyl sulfate is used in an aqueous solution to solubilize the hydrophobic monomer.

共聚胶束聚合[ 8 ]是最常用方法,其中表面活性剂,如十二烷基硫酸钠,是用在水溶液中,以solubilize疏水性单体。

The result indicated that we can get stable homopolymer and copolymer emulsion with uniform size distribution, when the ratio of sodium polyether sulfovinate modified polysiloxane to sodium lauryl sulfate was two, the quantity of the emulsifiers ranged from 6% to 8%, the quantity of initiator ranged from 0.4% to 0.8%, the stirring speed ranged from 200rpm to 250rpm, the polymerization temperature was 80 centigrade degree. As for the copolymer emulsion, the more the TRIS content in the monomers, the larger the water contact angle of their films became, namely, the surface energy of the copolymer films increased with the TRIS content in the monomers. In addition, the glass-transition temperature of the copolymers ranged from high temperature region to low temperature region with the increasing of TRIS content in the monomers.

结果表明:当乳化剂用量在6%~8%、改性乳化剂与十二烷基硫酸钠复配比为2:1、引发剂用量在0.4~0.8%、聚合温度为80℃、搅拌速度在200~250rpm时,得到的均聚物和共聚物乳液稳定,而且粒径分布均匀;对于共聚物乳液来说,随着单体中TRIS含量的增加,所得聚合物膜的水滴接触角增加,即其表面能增加:另外,随着共聚单体中TRIS含量的增加,共聚物的玻璃化转变温度从PMMA的Tg的高温区向PTRIS的Tg的低温区移动。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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