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To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Studies on the dormancy characteristics of Pinus bungeana seeds from 7 representative seed sources show that the difference of water permeability of seed capsule was very significant among seed sources.The seeds from northern part of distribution area had a much higher water permeability than from the south.

对7个具有代表性种源的白皮松种子的休眠特性研究表明,白皮松种子种皮的透水性在种源间差异明显,表现出随地理纬度的增高而逐渐增大的趋势,分布越是偏北的种源透水性趋于加强。

Soil available N was positively correlated with spore density of Salvia miltiorrhiza and Dioscorea opposite, and hyphal and total percent colonization of Adenophora borealis, while negatively correlated with arbuscular colonization of Aster tataricus; Soil available P exhibited a positive correlation with arbuscular colonization of Salvia miltiorrhiza and vesicular colonization of Dioscorea opposite, while a negative correlation with spore density of Platycodon grandiflorus and vesicular colonization of Atractylodes macrocephala; Spore density of Bupleurum Chinense had a positive affection with soil organic matter; Soil pH was negatively correlated with hyphal and total colonization of Adenophora borealis and spore density of Bupleurum chinense, while positively correlated with spore density of Adenophora borealis.

土壤碱解N与丹参和麻山药的孢子密度、北沙参菌丝定殖率和总定殖率呈显著正相关,与紫苑丛枝定殖率呈显著负相关。土壤速效P与丹参丛枝定殖率、麻山药泡囊定殖率呈显著正相关,与桔梗孢子密度、白术泡囊定殖率呈显著负相关。土壤有机质与北柴胡孢子密度呈显著负相关。土坡pH与北沙参菌丝定殖率和总定殖率、北柴胡孢子密度呈显著负相关,与北沙参孢子密度呈显著正相关。

More than 90% of East Asian haplotypes could be found in either Southeast Asian or Central-South Asian populations and show clinal structure with haplotype diversity decreasing from south to north.

这些新的结果与第二种情景相一致,它们意味着亚洲的主要基因库来自现代人类的某一单个的起初的进入,可能接着而来的是一个遍及整个大陆的从南至北的迁徙。

Now is the time for a journey deep in the dark Through icy chambers that lie deep within your heart The icy north winds chill to the very core Howling as they flow, a truth that's very sure Christ is Lord Christ is King Christ is God The North Wind In ancient texts that have been passed down through aeons There is a premise that just cannot be undone All we are and all we have is a gift of love A stone cold truth, stark as ice, revealed from above Christ is Lord Christ is King Christ is God The North Wind And so this love that you feel runs through your veins Feel your blood unthaw as you are born again His healing is coming you never will be the same Now is the time that you must lift high up his name Christ is Lord Christ is King Christ is God The North Wind Muscles, tissues, blood flow accelerates As you are restored into your intended state The love of God has restored your heart and soul The message of the North Wind, it has taken hold Christ is Lord Christ is King Christ is God The North Wind Lord, King, God x 4

现在是旅途深在黑暗中通过冰室摆在你的心深处冰冷的风冷却到北的核心嚎叫,因为它们流动,一个真相,非常肯定基督是主基督是王基督是上帝北风在古代文本已流传通过aeons 有一个前提,即不能被撤消所有我们和所有我们是一个爱的礼物石头冷真理,鲜明的冰,从上面显示基督是主基督是王基督是上帝北风因此这种爱,你会觉得他们穿过你的静脉感觉你的血液unthaw如你重生他的愈合是今后你永远不会是相同的现在是时候了,你必须解除了他的名字高基督是主基督是王基督是上帝北风肌肉组织,血液流动加快如你打算恢复到您的国家上帝的爱,恢复你的心脏和灵魂该邮件的北风吹,它已采取举行基督是主基督是王基督是上帝北风

But although German vintners have become more skilled at making dry wines with every vintage, I cannot put recommend dry 2005s as unreservedly as the traditional fruitier styles made in more northerly wine regions, especially Mosel-Saar-Ruwer, in this vintage.

不过,尽管德国葡萄酒商在酿制各个年份的干葡萄酒时技艺都更为娴熟,我还是不能推荐2005年的干葡萄酒,毫不客气,就像不推荐靠北的葡萄酒产区酿造的那种果味更浓的传统类型一样,尤其是这个年份的魔泽尔-萨尔-鲁维葡萄酒。

This image, captured by the MODIS on the Terra satellite on the morning of January 29, 2008, shows how extensive the unusual snow cover is. The image encompasses most of eastern China, except the northernmost provinces and the cloud-covered southern coast.

美国宇航局Terra地球观测卫星周二拍摄到的照片充分显示了中国的恶劣天气:由南到北的中国大城市密集的东部地区几乎全被大雪覆盖,反色照片上红色的区域都是大雪肆虐的地区,白色区域表示的是密布的云层。

Dandong city is located in China's northeast frontier, located in southeast of liaoning province, is China's largest city in the north of the border and coastal city, therefore has the advantageous natural conditions in dandong and precious historical culture, is a blend of natural scenery and humanistic landscape as one of the famous tourism city. More than 50 years ago, a song of "cock-a-hoop to, crossed the yalu" has inspired countless heroes, and impose their children for the Korean people's brother playing alongside, a common danger causes common action.

丹东地处中国东北部边陲,位于辽宁省东南部,是中国最大的边境城市和最北的沿海城市,因此丹东有着得天独厚的自然条件和珍贵的历史文化,是一座融自然风光与人文景观为一体的旅游名城。50多年前,一曲&雄赳赳,气昂昂,跨过鸭绿江&曾激励了无数英雄儿女为保家卫国,与兄弟的朝鲜人民并肩作战,同仇敌忾。

The my plant place geography position is extremely good, ground 107 just settle the segment, the transportation convenience, a hour can reach the downtown of a Chuang of stone,0.5 hours arrive the airport of a Chuang of stone, just outside a Chuang of stone that start construction is high-speed the wreath go into/ export in the my plant north door.

我的植物地方地理学位置极端好,地面 107 仅仅安顿片段,运输方便,一个小时能到达石头的 Chuang 的市中心区,0.5 小时到达石头的 Chuang 的飞机场,仅仅在开始建筑是高速的石头 Chuang 之外花圈在我的植物中进入/输出品北的门。

Coincidentally, agriculture branch kilometer in the East China Sea Garden, but also to the recent "concessions" in the form of lower prices, each unit prices as high as 10 million, while the King Tin Tin Ming Yuan kilometer is "flatly price" form, regardless of floor, regardless of a unified to 5,888 yuan per square metre price of external sale shortly gathered a lot of interest, as Meilin kilometer of the new century have driven Court last week on the occasion of the occupation on sale now at concessionary price of 4,480 yuan, and a large number of supporters access; Lo Wu District in the north and Porto Novo Tsui Chuk Garden in the land - the realization of the large residential area planning, development changed the landscape of the Lo Wu deficiency, owners in facilitating the full enjoyment of life at the same time bring the entire landscape area, especially the 4,600 yuan per square metre prices, many holding "Lo Wu complex" than those who moved, have set up projects in 70% of the sales of short-term rates

无独有偶,位于农科片区的东海花园,近期也以&优惠&的形式降低房价,每个单位降幅高达10多万元,而景田片区的天健名苑则以&一口价&的形式,不分楼层,不分朝向,统一以每平方米5888元的价格对外发售,短期内聚集了大量人气,同样,梅林片区的新世纪荔园阁也于上周入伙之际以特惠价4480元发售现楼,获得大批拥趸;而位于罗湖区翠竹北的新港鸿花园则在寸土寸金之地实现了小区的大型豪宅规划,一改罗湖楼盘景观不足的缺憾,使业主在便利生活的同时充分享受小区景观带来的愉悦,尤其是每平方米4600元的优惠价格,令不少难舍&罗湖情结&的人士心动不已,纷纷落订,项目短期内突破70%的销售率

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推荐网络例句

I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。