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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

I love the amniotic fluid when it spills out of the bag. I love the kidney with its painful gallstones, its gravel and whatnot; I love the urine that pours out scalding and the clap that runs endlessly; I love the words of hysterics and the sentences that flow on like dysentery and mirror all the sick images of the soul; I love the great rivers like the Amazon and the Orinoco, where crazy men like Moravagine float on through dream and legend in an open boat and drown in the blind mouths of the river. I love everything that flows, even the menstrual flow that carries away the seed unfecund. I love scripts that flow, be they hieratic, esoteric, perverse, polymorph, or unilateral. I love everything that flows, everything that has time in it and becoming, that brings us back to the beginning where there is never end: the violence of the prophets, the obscenity that is ecstasy, the wisdom of the fanatic, the priest with his rubber litany, the foul words of the whore, the spittle that floats away in the gutter, the milk of the breast and the bitter honey that pours from the womb, all that is fluid, melting, dissolute and dissolvent, all the pus and dirt that in flowing is purified, that loses its sense of origin, that makes the great circuit toward death and dissolution.

我爱从羊膜中溅出的羊水;我爱生着引起痛苦的的结石、肾砂和诸如此类东西的肾脏;我爱撒出的热呼呼的尿和久治不愈的淋病;我爱歇斯底里的疯话、像拉痢疾一样一泻而出的句子和灵魂全部病态的映像;我爱亚马逊河和奥里诺科河这样的大河,那儿摩拉瓦基乃之流的狂人在一只无顶的小船上漂过了梦和古老的传说,淹死在瞎眼的河口中;我爱流动的一切,甚至爱女人来月经时流出的血,它冲走了生育能力不强的精子;我爱会流动的手稿,不论它们是用象形文字写的、深奥的、反常的、多形体的或是单边音的;我爱流动的一切,一切其中有时间的和适当的东西,它们把我们带回永远不会结束的开始中,即先知们激烈、令人狂喜的猥亵,宗教狂的智慧,牧师和他的橡皮连祷文,妓女的下流话,从排水道里漂走的唾液,乳房里的奶汁和子宫里流出的带苦味的蜜水,以及一切流质的、溶化的、放荡的和有溶解力的,所有在流动中得到静化的脓和脏物,那些失去其出身意识的东西和那些将大循环驱向死亡和瓦解的东西。

The presented papers in the XXIX IULTCS Congress and 103rd ALCA Annual Meeting were reviewed The papers covered the following field tanning mechanism treatment of tannery effluents environmental regulation biodegradation of leather and leather chemicals utilization of leather waste clean technology of leather making preservation of raw hides and microbial protection of leather novel leather process and materials physical and mechanical properties of leather new analytical and detecting equipments and method .

本次会议论文主要涉及:皮革鞣制机理的研究,皮革行业的污染治理,皮革行业环境保护政策及动向,皮革及其化学品的生物降解,胶原及制革废弃物的资源化利用,制革清洁生产技术,生皮的清洁化防腐保存和皮革的抗菌防霉,制革新工艺和新材料,皮革的物理机械性能及新的皮革分析检测设备和方法等。

The presented papers in the XXIX IULTCS Congress and ALCA Annual Meeting were reviewed The papers covered the following field tanning mechanism treatment of tannery effluents environmental regulation biodegradation of leather and leather chemicals utilization of leather waste clean technology of leather making preservation of raw hides and microbial protection of leather novel leather process and materials physical and mechanical properties of leather new analytical and detecting equipments and method .

本次会议论文主要涉及:皮革鞣制机理的研究,皮革行业的污染治理,皮革行业环境保护政策及动向,皮革及其化学品的生物降解,胶原及制革废弃物的资源化利用,制革清洁生产技术,生皮的清洁化防腐保存和皮革的抗菌防霉,制革新工艺和新材料,皮革的物理机械性能及新的皮革分析检测设备和方法等。

Reduction of ovule number as a tendency of speciation in the genus is substantiated by the occurrence of tri_ovulate cones coupled with comprehensive characters of the genus. The metamorphic patterns as well as the leaf nature of the outer envelope indicate that female cones of Ephedra are compound while the female reproductive units of the ovulate cone have been reduced from secondary reproductive shoots similar to those of cordaites by phylogenetic transformation, fusion and reduction. Each fertile bract together with its axillary female reproductive unit composed the Bract Scale and Seed Scale Complex of Ephedra .

基于返祖性畸形三胚珠球花的出现及其他形态特征,认为麻黄属胚珠数目减少是该属内物种特化的一个趋势;利用雌球花发育的畸形现象及外珠被的形态学本质提出麻黄属的雌球果为复轴性构造,雌性生殖单位是由类似于科达类的次级生殖枝构造经系统发育变态、融合、简化成现存的生胚珠构造式样,每一可育苞片及其腋内雌性生殖单位共同组成了麻黄属的苞鳞_种鳞复合体。

Dyce life underwent a radical social change after the industrial revolution; his concerns regarding the cultural, religious values in the transitional society made his works significant when it comes to the Victorian paintings.

戴斯生逢工业革所伴随而来的时代巨变,体察工商业文化下的社会、宗教等价值变迁,作品强调反映当时的宗教境况,重视绘画精神性与教化功能,是以让他的绘画能在性灵匮乏的年代提供抚慰人心的功用,也因而使他跻身维多利亚时期的重要艺术家之林。

This thesis analyzes theoretical base about the back-to-life curriculum in kindergarten from the angle of life, life and children, life and curriculum, which put forward the point that life is definitely to occupy, enjoy, assimilate and create the material culture, spiritual culture and institutional culture of human beings, and life is an motile activity which exists, develops with a person"s life and which can realize a person"s life values. The thesis also advances the view that the childrens mental and body characters decides that their study style is experiential, integrated, gameful, active and passive. All these make the life world of children become a unique one. Life character is the most basic one of the curriculum in kindergarten.

本文从生活、生活与儿童、生活与课程等角度对幼儿园课程回归生活进行了理论分析,提出:生活就是占有、享受、内化并创造人类物质文化、精神文化和制度文化,围绕人的生命存在和发展,实现人生价值和意义的能动的活动;儿童的身心特点决定了他们的学习具有直接经验性、整体性、游戏性等特性,这就使儿童的生活世界成为一个独特的世界;生活性是幼儿园课程最基本的特性。

Effect of protein and lipid in rice on starch physicochemical characteristics, difference between the region of non-chalkiness and chalkiness of rice, dynamic variation of cell structure and CaM distribution on rice spike were expounded in this study. It was found that protein and lipid in rice had important effect on starch gelatinization, there were distinct differences between the region of non-chalkiness and chalkiness of rice, vascular bundle system in rice spike was changed during grain filling, difference quality of different position of rice spike was more distinct in dense spike rice for more powerful vascular bundle system, the development of rice seeds was corresponded to pericarp development, difference quality of different position of rice spike was also related to transport function of rachilla besides activity of "source", and calmodulin relative activity was closely related to grain filling velocity and enginery properties.

通过实验发现:稻米中蛋白质、脂肪对淀粉的糊化过程有着较大的影响;精米的非垩白与垩白部位以及心白与腹白部位之间在蛋白质、氨基酸含量及组成方面存在着明显差异:水稻穗部大维管束面积以及主穗轴维管束数目在灌浆过程中存在明显的动态变化趋势;密穗型水稻强势粒的维管组织比散穗型水稻发达,致使其强、弱势粒维管组织差异明显加大;果皮的发育与籽粒发育状况是相适应的:强势粒和弱势粒在灌浆生理方面的差异,不仅表现在籽粒本身的&库活性&上,而且也与其着生小穗轴的输导功能有关;CaM相对活性与水稻灌浆速率、机能特征间存在着密切联系。

Under the condition of laboratory incubation,the C loss of organicmaterials during their mineralization was greater than their N loss,inducing ahigher residual amount of organic organic N.During the decay of maize andrice staw with a wide ratio of C/N.soil microbes could fix N,but the fixationrate was quite low,only 0.2-0.9g N/assimulated 100g organic C.

在实验室培养条件下有机物料在矿化分解过程中C的损失总是大于N的损失,因此有机N的残留率明显高于有机C的残留;且由于灰分总量不变,有机物料的残留率也略高于有机C的残留率;〓比较宽的玉米秸和稻秸在腐解过程中发生了自生固N作用,但其固N效率仅为C:N=100:0.2-0.9,即每同化100g有机C,只能固定

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞,加入莫能霉素,用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间,比较其胞内表达白细胞介素-2(IL-2)/白细胞介素-4(IL-4)的细胞百分率,确定最适诱生条件;用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验,确认McAb、bio-SAM IgG和HRP-SA的最适浓度,根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

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