化学过程

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

By the optimized condition of Sol-Gel process,the film has good quality was prepared. The performances of the electrode have been tested in the paper. The effects of solution stability,temperature and interferent have also been studied. Cyclic voltammetry was used to investigate formative process of sensitive film.

实验对溶胶和凝胶化过程中Sol储备液的配比、陈化时间、提拉速度及膜的干燥条件等因素对成膜质量的影响进行了优化；测试了Sol-Gel化学修饰碘电极的性能，并研究了待测液的稳定性、温度和共存物等因素对测定的影响。

Dye can bring a little color to life, most clothing is color with dyes, modern manufacture dyes can be costly, natural dyes from plan t and animal products have been used since ancient times, so this week, we describe by natural way to dye world, the advice comes from information written by Jenny Tim of the intermedial technology development group in Briton, this anti group is now called practical action, there are several messages to put dye onto material, the vent message for example, can be used to dye wool with onion skins, for this example, use 100 grams of natural wool, the wool must be clean, leave it overnight in water and liquid soap, then wash it with clean water that is a little warm, gently out the water, a solution called a moderant is used in the dying process, a moderant helps fix the dye to the material, traditionally moderants were found in nature, wood ash is one of example, but chemical moderants such as ironare popular today, iron is so would in many stores, it is often mixed with queen of daughter, a finding powder commonly used in cooking.

使用染料可以为生活带来更多的色彩。许多的布料都是通过染料来上色的。现代工业制造的染料通常都非常的贵，从古时起，人们就知道从植物或者动物产品中提取天然染料。这周，我们就来讨论一下如何用天然的方法来给羊毛着色。这一建议来自英国中级技术发展组的Jenny Dean的信息。这一古老贫穷的小组现在被称为实用性活动。把染料加到材料上有几种方法。其中之一是大桶法。举例来说，就是用洋葱皮来染羊毛。这一例子中，用100g的天然羊毛。羊毛必须清洗。用液体肥皂浸泡过夜。然后用温的干净的水冲洗它。轻柔地把多余的水分压挤出来。一种叫做媒染的溶剂也用于染洗过程。媒染是用来固定染料和材料的。传统的方法是，媒染是来天然的，比如木灰。但是像明矾这样的化学媒染在今天也是很流行的。明矾在许多商场都有出售。它通常和一种经常被用于烹调的粉状物-酒石混在一起。我们用多一点点的辛苦，来交换多一点点的幸福

From the experiment results,it is concerned that the thin film was composed of many small particles(most of them was with a size between 20 and 30 nm)uniformly deposited and intervein on the substrate surface. Some large particles were tightly embedded into the thin film.The deposition rate increased by increasing the precursor flow rate and the discharge power.

以上的分析结果表明：常压等离子化学气相沉积得到的薄膜是一种由众多小颗粒(其中主要为尺度在20-30nm的颗粒)的颗粒串联形成的网孔结构，并且在薄膜生长过程中一部分的颗粒被包埋在其中，薄膜颗粒分布较均匀。

Electrodeionization or EDI, is a continuous and chemical-free process of removing ionized and ionizable species from the feed water using DC power.

电或电子数据交换，是一个持续的和化学的过程中消除电离和ionizable种给水用直流电源。

Results There were about 15 kinds of chemical poisons including vinyl chloride,polyvinyl alcohol,isoparaffin,polyvinylacetate,sodium hydrate,ammonia,acid hydroc,methanol,diesel oil,carbon monoxide,carbon dioxide,sulfur dioxide,nitrogen oxides,ozo...

结果在聚氯乙烯的生产过程中，作业场所存在化学毒物较多，主要包括氯乙烯、聚氯乙烯、聚乙烯醇、过氧化物、异链烷烃类、聚乙烯乙酸酯、氢氧化钠、氨、盐酸、甲醇、柴油、一氧化碳、二氧化碳、二氧化硫、氮氧化物、臭氧、二氧化锰等。

The electrospray ionization mass spectrometry was used to study the fragmentation pathway of kaempferol. The fragmentation pathway of kaempferol was also theoretically studied by means of Density Functional Theory.

采用电喷雾质谱技术对山奈酚的质谱过程进行了表征，并用量子化学方法对山奈酚的质谱裂解途径进行了理论研究。

A chemical bonding approach for kinetically predicting the growth shape of single crystals is proposed.An attempt was made to find the relation between crystal morphology and both internal and external factors.

本论文从化学键合角度出发对晶体生长过程进行分析，综合考虑了晶体内部结构和生长环境因素对晶体最终形貌的影响，提出了一个由动力学因素控制的形貌预测模型。

Of Fe , Cu in the metal phase and Mg, Si in the silicate phases to understand the processes of mixing of metal and silicate phases and further decipher the mechanism of formation of mesosiderite;(2) to study in detail the geochemical characteristics of each phase in the meteorite, to provide necessaryevidence for the model of formation of mesosiderite;(3) to study and update in detail the composition of each phase in the meteorite, and provide a new and complete set of data for understanding the mechanism of formation of mesosiderite.

Cu和硅酸盐相中的Mg， Si等中等挥发性元素的稳定同位素进行分析，判断中铁陨石的金属相和硅酸盐相混合过程的环境特征，进而阐明中铁陨石的形成机制；（2）对这块陨石的各相进行详尽的地球化学分析，通过这些相的地球化学关系判断中铁陨石的形成机制，对中铁陨石的形成模型提供必要的制约和证据；（3）全面更新对中铁陨石各矿物相化学成份的认识，为中铁陨石的成因之谜的全面揭开提供最新最完整的数据。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。