化学生物学

1Chen kangli, 2yan meijuan, 1zhang ying （1department of biochemistry and molecular biology, shanghai medical college, fudan university, shanghai, 200032; 2jiangsu key laboratory of neuroregeneration, nantong university

作者单位：1复旦大学上海医学院生物化学与分子生物学系，上海 200032； 2南通大学江苏省神经再生重点实验室

2Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Seoul 130701

1吉林大学第一医院神经外科，长春 130021；2庆熙大学医科大学生物化学与分子生物学教研室，首尔 130701

By the methods of molecular biology, enzymology, chemistry, physics the project was carried out. The genes encoding leucyl- and arginyl-tRNA synthetase , and tRNA and tRNA from Escherichia coli were cloned and overexpressed in E.

以大肠杆菌亮氨酰tRNA合成酶和精氨酰tRNA合成酶为对象用分子生物学、酶学、酶动力学、化学、物理学的方法进行该项研究。

The reconstruction of past variations in lake level, water chemistry and limnological conditions may provide key site information on the post-glacial climate change of the region.

重建黄旗海水位、水化学和湖泊生物学变化历史可为研究我国夏季风边缘区冰后期气候变化提供重要环境记录。

As a result of what is now know in physics and chemistry, scientists have been able to make important discoveries in bio logy and medicine.

物理学和化学的一个成果是使得科学家们能在生物学和医学上获得重大发现。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

According to the chemical composition and biological characteristics, the hernia repair materials are divided into three kinds: nonabsorbable, absorbable and compound materials.

根据材料学的化学成分和生物学特性可将疝修补材料分为不可吸收材料、可吸收材料和复合型材料3大类。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

The oversaturation of cholesterol in bile results in the formation of cholesterol stones. The liver is the organ which synthesizes cholesterol and secretes bile. Liver lipid metabolism research helps to explain the mechanism of cholelithiasis. Utilizing biochemical and biomolecular technology, we have done experimental work as follows: Analyse the ingredient changes of blood and bile samples from patients.

肝脏是合成胆固醇、分泌胆汁酸的器官，对肝脏胆固醇代谢的研究有助于阐明胆石症形成的原因，本文用生物化学和分子生物学的方法，分析了病人血液、胆汁中各种脂类成份的变化，以及肝脏胆固醇合成限速酶3-羟-3-甲戊二酸辅酶A还原酶（HMG-CoA Reductase）、胆汁酸合成限速酶7α-羟化酶（7α-hydrolase）的基因表达。

The expression status of 〓 andRB protein in the tissues of normal, chronic pancreatitisand pancreatogenic carcinoma were detected by immunohis-tochmical staining. Also, the relationship be-tween the feature of cancer and prognosis was analysed in combi-nation with clinical data.

采用免疫组织化学染色，检查胰腺癌、慢性胰腺炎、正常胰腺等组织的〓蛋白和RB蛋白表达状态，并结合临床资料，将其与胰腺癌的生物学特性及予后的关系加以分析。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。