化学生物学

Because of this crucial function in the plant's reproductive cycle, pollen is the objectof biochemical and molecular biological research.

正是由于花粉的这一重要作用，使其成为生物化学和分子生物学领域研究基本规律性问题的首选对象。

Intraoperative spillage of tumor cells in surgery for rectal cancer.

直肠癌生物学行为的组织化学研究。

This is the webpage The Journal of Steroid Biochemistry and Molecular Biology.

这是《类固醇生物化学和分子生物学杂志》的网页。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

According to the chemical components and biological characteristics, the materials for the repair comprise unabsorbable materials, absorbable materials, composite materials and biological materials.

疝修补材料依据化学成分和生物学特性，可以将其分为不可吸收材料、可吸收材料、复合型修补材料及生物型材料。

Cardiac hypertrophy is one of adaptive responses of the heart to a variety of pathological stimuli, including hypertension, myocardial infarction, congenital heart disease, heart valvula disease, and perturbations in sarcomeric function due to altered expression or mutations of contractile proteins. Increase in wall tension and hormonal stimuli are thought to directly activate various membrane-bound receptors and signal transduction cascade within cardiomyocytes resulting in the activation of immediate early genes and responsive genes such as c-jun, c-fos, c-myc, egr-1, ANF, BNP. Then the expression of construction proteins such as MLC-1, skeletal α-actin was unregulated.

中文题名hhlim基因的表达调节及其与心肌细胞肥大发生之间的关系副题名外文题名 hhlim gene expression regulation and its role in development of cardiomyocyte hypertrophy 论文作者郑斌导师温进坤韩梅学科专业生物化学与分子生物学研究领域\研究方向学位级别博士学位授予单位河北医科大学学位授予日期2003 论文页码总数106页关键词心肌细胞肥大基因表达心肌肥厚 hhlim基因馆藏号BSLW /2003 /R542.2 /33 心肌细胞肥大是高血压、心脏瓣膜病、急性心肌梗塞及先天性心脏病等临床常见疾病的一种并发症，是心肌细胞对多种病理刺激的一种适应性反应。

In the thesis, the systematic relationships of Acorus together with Araceae and Typhales are discussed on the basis of features from micromorphology, anatomy, pollen morphology and floral development, with the aid of the data from their embryology, cytology, chemotaxonomy and molecular biology.

本文用形态学、解剖学、孢粉学及花器官发生等方法，并借助胚胎学、细胞学、植物化学及分子生物学等方面的资料，比较研究了菖蒲属与天南星科及香蒲目的系统关系。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

In the field of biology the paper dicusses mating,parasite,developing,oviposition,etc.of Aphidius gifuensis and effect on its host,also ecologically dicusses its natural enemies and effect of weather,growth of tobacco,insecticide on Aphidius gifuensis.

生物学方面主要论述了烟蚜茧蜂的交配、寄生、发育、产卵、体色、性比、越冬、成蜂寿命及其对寄主生命活动的影响：生态学方面主要论述了烟蚜茧蜂的天敌以及气候因子、烟草长势和化学农药对其的影响。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。