化学法

The sensitivity of CEA by immunofluorescence examination and LSCM is useful for the diagnosis,differential diagnosis of meningeal carcinomatosis.

联合应用免疫荧光细胞化学法和LSCM检测脑脊液细胞中CEA的表达可为脑膜癌病的诊断提供可靠依据。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

It was regarded as being widely used chemical indudstry material in 21 century and more and more researchers pay attention to that.At present, the primary method to produce 1,3-propanediol is chemosynthesis method.

目前生产1，3—丙二醇的方法主要是化学法，由于化学法生产成本高，且对环境污染大，因此微生物法生产1，3—丙二醇成为当前研究的热点。

The oil sewage disposed process is a complex disposal technique. The disposal process is more difficult for the change of PH Flux temperature of raw water and the other polluted quality. At present the disposed water by the system of oil of sewage is unstable and high cost. Now the method for the oil of sewage have physics chemistry physical chemistry and biology etc. In the every disposed method has its own specially, Basis of the oil sewage condition of power plant. The text use chemic method. Another word is method of Flocculation-deposition. Injecting Dosage into sewage. Making small suspends grain and the electrophorus colloid gather into bigger colloid and deposit. Separated it from water and purify the oil sewage. Make disposed oil water up to grade.

含油污水处理工艺是一个复杂的处理过程，在处理过程中由于受到原水浊度、PH值、配水流量、原水温度和其他水质污染等因素的影响，处理难度相当大，现时的含油污水处理系统普遍存在处理后的水质不稳定和处理成本高等问题，国内外含油污水处理采用的方法有物理法、化学法、物理化学法和生物法等，每种处理方法都有自己的特点，结合电厂含油污水的情况，本文主要采用化学法，具体来说是采用絮凝沉淀的方法，通过向废水中投加混凝剂，使细小的悬浮颗粒和带电胶体微粒聚集成较大颗粒而沉淀，使其与水分离，使含油污水得到净化，使处理后的废水各项指标达到排放标准。

Combination of enzymolysis and alkaline hydrolysis was superior t...

而在水浮莲膳食纤维的持水力和膨胀性方面酶碱法优于化学法。

Methods All cerebrospinal fluid cells (control group and meningeal carcinomatosis) were stained with CEA by immunofluorescence, scanogram and fluorescent intensity stereophotography were obtained and finally fluorescent values of CEA were individually measured by LSCM.

应用免疫荧光细胞化学法以CEA标记脑膜癌细胞及对照组脑脊液细胞，采用LSCM技术获得脑膜癌细胞扫描图像并定量测定CEA荧光强度值。

A comparison of three chemical analytical methods-gravimetric method of barbituric acid, hydroxylamine hydrochloride method and bro-mide-bromate method-for determination of furfural content was studied.

测定糠醛含量的方法很多，日前同际标准化组织和各国的糠醛标准检验方法均采用化学法，成品糠醛内含有各种微量的有机杂质，据报道。

Many methods for making nanoscale materials were developed in the past few years, such as colloidal crystallization, monolayer deposition, multiplayer casting, molecular crosslinking, complementary interaction, patterned etch pits, template-mediated and DPN, most of these are complicated and costive.

在过去的几年中，合成纳米复合材料的方法已取得了极大的进展，如胶体结晶法、单层膜沉积法、多层膜浇铸法、分子交联法、互补反应法、图形刻蚀法、模板化学法、DPN法等，但是这些方法大多操作耗时、费力。

Using the MTT assay, agarose gel electrophoresis, transmission electron microscope, TUNEL method and immunohistochemistry (PCNA, bcl-2),it was studied in this experiment that the inducing APO, anti-proliferation and affection of expression of bcl-2 gene before and afterAPO of the Rubia Cordifolia L with the 5-fluorouracil(5-fu) for MGC-803 cells of gastric carcinoma.

本实验通过MTT法、琼脂糖凝胶电泳法、透射电镜观察法、TUNEL法、免疫细胞化学法（PCNA、bcl-2），研究了茜草提取物联用5-氟尿嘧啶（5-fluorouracil，5-Fu）对胃癌MGC-803细胞株的诱导凋亡、抗增殖作用及凋亡前后bcl-2基因表达的影响。

By comparison, there are many advantages associated with enzymatic approach: the reaction can be carried out under mild conditions, the process is environmentally friendly and high quality product can be achieved.

相对于化学法，生物酶法合成1，3-DAG具有反应条件温和、环境友好、产品质量好等优点，故近年来利用酶法合成1，3-DAG越来越受到关注。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失