化学方法

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

The chapter 3 focuses on the design and synthesis of new thiazole derivatives.

论文第四章是噻唑衍生物的固相合成，设计并开发了用组合化学方法合成噻唑衍生物的新方法。

Methods The anticoagulating activity of Bombys batryticatus was detected by TT assay and the main components associated with this activity were determined by classic chemical assayes on different fractions of crude and prepared Bombys batryticatus.

方法用凝血酶时间和经典化学方法为指标测定生、制僵蚕以及制僵蚕的不同部位的抗凝血活性大小以及总氮、铵态氮、蛋白质和草酸铵的含量。

Objective To study the relationship between IL-1,MMP-13and pathogenesis of knee osˉteoarthrosis.Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

目的 探讨白细胞介素-1（IL-1）、基质金属蛋白酶-13（MMP-13）在膝关节骨性关节炎发病中的作用方法提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

Spinal cord injury models were made in 60 wistar rats and the dynamic expression of bromodeoxyuridine and polysialylated ependymal cells adhesion molecule were determined by immuno-histochemisty.

方法］Wistar大鼠60只，制作大鼠脊髓全横断损伤模型，根据伤后不同时间点分组，应用免疫组织化学方法动态检测脊髓内5-溴脱氧尿嘧啶、多唾液酸神经细胞黏附分子的表达。

Methods The surviving was detected by immunohistochemical assay in urine sediments samples of the 75 cases of bladder tumor, 2 Glandulose cystitis, and 10 normal control.

方法用免疫组织化学方法检测膀胱肿瘤75例、腺性膀胱炎2例、正常对照10例的尿沉渣Survivin蛋白的表达。

Sixty-three cases of AFP-positive gastric carcinoma were divided into four subtype: hepatoid type 22 cases (34.9％), fetal gastrointestinal type 15 cases (23.8%), and mixed type of the hepatoid type with enteroblastic differentiation to be 24 cases(38.1%), and yolk sac tumor-like type 2 cases (3.2％).

应用光镜、电镜、组化和免疫组织化学方法，对87例AFP阳性胃癌中临床病理资料较完整的63例进行病理组织形态观察，并用2例3个月妊娠的胚胎胃肠组织以同样方法行相关的对比研究。

For two or three kinds of isotopic composition of elements, mass spectrometry is superior to chemical methods, and other physical methods.

对由两、三种同位素组成的元素，质谱法优于化学方法和其他物理方法。

The paper analyzed influence of temperature upon metatitanic acid density and has found a correction method for densitomeler using re-gression equation of temperature and density ,they determined and verified the empirical constant for conversion equation of the slurry demsity and concentration with physical and chemical method.

分析了温度对偏钛酸比重的影响规律，找出利用温度和比重的回归方程校准比重计的方法，同时利用物理和化学方法，确定和验证了浆料比重与其浓度换算规律的经验常数，结果令人满意。

Methods A total of 53 SRE specimens and 8 nomal nephridial tissues were obtained. Immunohistochemistry method was used to test protein expression of survivin. Semi-quantitative RT-PCR was used to measure survivin mRNA levels.

收集53例肾透明细胞癌组织及8例正常肾组织，以免疫组织化学方法检测survivin蛋白表达；半定量RT-PCR方法，检测肾透明细胞癌中survivin mRNA表达水平，与正常肾组织进行比较分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失