化学方法

Results: Hippocampal neurons were founded to be maturated by the methods of morphology and immunohistochemistry.

结果 培养8天后的海马神经元经形态学和组织化学方法观察证实神经细胞发育成熟。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长，呈成纤维细胞样形态，经5一aza诱导后细胞体积增大，变为球状或者长杆状，4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性，10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高，但20umol·L一'5一aza组约50%细胞死亡。

Oxidative and nitrative injury in human umbilical vein endothelial cells (ECV304) induced by high glucose and exogenous nitrating agent were detected by cell biological and chemical methods.

采用细胞生物学及化学方法，检测了高浓度的糖和外源性硝化试剂作用于人脐静脉内皮细胞(ECV304)后所导致的细胞蛋白氧化损伤及硝化损伤。

Then sandblasting, flame or chemical means, and improve the surface activity.

然后用喷砂、火焰或化学方法处理，提高表面活性。

The tumor-bearing mice received corresponding treatments for 15 consecutive days, after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. The tumor mass was also weighed and the tumor inhibition rate calculated.

连续用药15d。第15天处死小鼠，计算胸腺指数、脾指数；剥离瘤块称重并计算抑瘤率；用免疫组织化学方法检测各组荷瘤小鼠肿瘤组织的增殖细胞核抗原的表达强度。

Methods: We transplanted neural stem cells from embryobrain of rats in hippocampi of AD model rats transecting fimbria fornix with or without FG. 30 days after transplanting, test these AD models' behavior then inspect living and differentiation of NSCs by immuno -fluorescence.

体外分离培养大鼠胚脑神经干细胞，用纤维蛋白凝胶为支架移植入穹隆海马伞切断AD模型鼠海马，行为学测试后用免疫组织化学方法检测神经干细胞存活和分化情况。

Experiment 1, let rats acquire taste preference to quinine solution, and take account of β-EP-immunoreactive neurons in different nucleuses of hypothalamic areas, separately adopted taste preference learning training and immunohistochemistry; Experiment 2, Added banana essence into quinine solution to discuss the connection between flavor preference learning and the expression of β-EP; Experiment 3, To research endogenous opiate like substances contribute to the establishment of CTP and influence the behavior of taste preference by naloxone was i.p.

实验一，采用味觉嗜好学习训练和免疫组织化学方法，让大鼠获得对奎宁的味觉嗜好，计数了下丘脑不同核团内β-内啡肽免疫反应细胞数；实验二，向奎宁溶液中加入香蕉气味，探讨风味嗜好学习与下丘脑β-内啡肽表达的关系；实验三，腹腔内注射纳络酮，探讨内阿片肽对CTP建立及味觉嗜好行为表达的影响。

In our experiments, the hafnium films were deposited on molybdenum substrates by radio frequency magnetron sputtering technology, and the barium oxide layers were prepared by the chemical method to simulate the grid structure of traveling wave tubes. Then, the surface phase and compositional change in the grid was studied under high temperature by annealing from 900K to BOOK, which can be used in explaining the suppression mechanism of hafnium film.

实验中，利用射频磁控溅射技术在Mo基底上制备了Hf膜，并利用化学方法制备了BaO涂层以模拟行波管栅极结构，随后在N_2保护下，通过在900K到1300K范围内退火，研究栅极处于高温工作环境下表面相结构和成分的变化，以此解释了Hf薄膜抑制栅极电子发射的工作机理。

Barium sulphate precipitated is the main analysis of production through chemical composition, its main compounds of barite ore material is natural barite.

沉淀硫酸钡是通过化学方法生产的主要成分为硫酸钡的化合物，其主要原料是天然重晶石。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。