化学方法

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

The expression of AT1 and AT2 receptor was examined in ureteric bud cell of the embryonic mouse, including embryonic days 12, 14, 15, 16 by immunohistochemical technique.

采用免疫组织化学方法检测胚龄12，14，15，16 d小鼠肾脏发育早期输尿管芽分支中血管紧张素Ⅱ受体1和受体2的含量。

Results; The histological change of kidney iNOS was markedly increased in the IR+NS group. The levels of serum creatinine and blood uria nitrogen were significantly upregulated.

应用免疫组织化学方法和图像分析系统检测肾组织中INOS的表达和分布，比较各组血清肌酐、尿素氮，同时观察肾脏组织形态学改变。

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

应用蛋白印迹及免疫组织化学方法检测CD147在宫颈癌和正常宫颈组织中的表达。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Methods The heat shock protein 70 (HSP70) of rabbit skin was measured by the immunohistochemcial method and the differences of the HSP70 expression in rabbit governor vessel and its bilateral sides skin were compared at 4h and 16h after depilating treatment.

免疫组织化学方法检测热休克蛋白70(HSP70)，比较经脱毛膏脱毛处理后恢复期4h、16h皮肤HSP70表达的差异以及督脉线及其两侧旁开对照点HSP70表达的差异。

Methods The expression of C-myc in 40 cases of gliomas was examined with immunohistochemical technique.

应用免疫组织化学方法检测40例脑胶质瘤组织标本中的C—myc蛋白表达情况。

The expression was higher in undifferentiated NPC than in keratinizing squamous cell carcinoma and non-keratinizing carcinomas.

用免疫组织化学方法检测CD25细胞在鼻咽癌组织和鼻咽慢性炎症组织中的表达情况。并用组织原位杂交技术检测EB病毒对其表达的影响。

According to scientific analysis, the coatings are natural lacquers .

经过化学方法和光谱分析，其涂料为天然漆。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。