化学方法

Positive expressive rate of cyclin D1 in colorectal carcinoma was 60.34%（35/58）, which was higher than that in tumor sideward mucosa and normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测其对HepG-2细胞周期分布的影响，同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达，观察其诱导肝癌HepG-2细胞凋亡的作用。

Their structures were elucidated on the basis of detailed spectroscopic analysis and chemical methods.

通过详细的波谱学分析及化学方法，对分离到的化合物进行结构鉴定，其中有6个新化合物。

The inducible nitric oxide synthase is obviously expressed in speen of rats under the predator stress, suggesting important function for iNOS in speen physiology. However, the expression and subcellular localization for NOS in speen development and differentiation are largely unknown. In the present study, the changes in iNOS activity in spleen of rats under predator stress were studied to explore the role of iNOS in the decreased immunoactivity of the stressed rats. The changes in iNOS were observed with immunobistochemical method, and the contents of NOS and NO in spleen with biochemical method.

在捕食压力下，大鼠脾脏诱导型一氧化氮合酶明显表达，说明iNOS起到了重要的生理功能，然而，NOS的表达及在亚细胞水平的定位以及相应的差异变化等都很不明确，本试验采用捕食应激模型，利用免疫组织化学方法和生化测定，检测心理性应激对大鼠脾脏iNOS活性的影响及探讨这种影响与应激动物免疫力下降之间的关系。

Localisation of testosterone and androgen receptors was investigated in different stages of the spermatogenetic cycle of Rana chensinensis, using immunocytochemistry.

用免疫细胞化学方法检测了睾酮和雄激素受体在中国林蛙生精周期中不同时期精巢内的表达定位。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

METHODS: The neonatal and adult rats facial nerve was ligated at stylomastoid foramen.

于新生及成年大鼠茎乳孔处结扎面神经，于3， 7， 14， 21， 28， 56， 84 d采用NADPHd组织化学方法观察NADPHd阳性细胞在面神经核中的变化。

After the type 1 diabetic encephalopathy rat models were set by streptozotocin for 5 days, insulin and saline were used respectively to treat the related model rats for 55 consecutive days. Morris water maze and bromodeoxyuridine immunohistochemistry were used to detect the changes in spatial memory and learning, and the neural stem cell proliferation in the subgranular zone of hippocampal dentate gyrus of each rat group.

在用链脲佐菌素建立1型糖尿病脑病模型后的第5天，分别用胰岛素和生理盐水替代治疗55天，再通过Morris水迷宫测试及5-溴脱氧尿嘧啶（BrdU，神经干细胞DNA合成的标记物）免疫组织化学方法，分别观察和比较各组大鼠SGZ神经干细胞增殖和空间学习记忆的动态变化。

The usual chemical process involves making succinic acid from crude oil or natural gas.

一般的化学方法需要从原油或天然气制取琥珀酸。

We observed the effect of chronic hypoxia on intracelular levels of CGRP in pulmonary endocrine cells of SD rats by means of immunocytochemistry and supraoptimal dilution method.

我们用免疫细胞化学方法及超稀释法观察了慢性缺氧对SD大鼠肺中神经内分泌细胞内降钙素基因相关肽的含量的影响。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。