化学方法

Methods: The expressions of CGRP and VEGF in the wound tissue were detected by immunohistochmisty technique and MIAS image analysis system.

应用免疫组织化学方法、图像分析技术动态检测大鼠创伤组织中CGRP，VEGF的表达。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示，贻贝棘尾虫休眠包囊中，在胞质嗜锇粒和拟糖原粒区，酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上，葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

Were comparativey studied using immunocytochemical tech niques-peroxidase antiperoxidasemethod.These positive immunoreactive systems were compared with the neuroanatomical data on neuropile organization and single nerve cell type in the bee's brain.

通过免疫细胞化学方法－PAP法，研究了中华峰和意大利峰脑和视叶中乙酰胆碱、5－羟色胺（5－HT）、γ－氨基丁酸免疫反应阳性神经元胞体和纤维的形态、染色强度、分布和各种神经丛之间的联系。

Objective To study the neuropathological features of transgenic model for Alzheimer's disease by pathomorphological and immunohistochemical methods.

目的 通过组织病理学和免疫组织化学方法，验证阿尔茨海默症转基因动物模型。

Methods: Based on the theory of "kidney produces sui", we set up MCAO rat model. Five indexes including Nestin, PSA-NCAM, MAP-2, GAP-43 and SYN38 were measured in situ hybridization and immunohistochemical methods to evaluate the effects of Fujian Tablet on the proliferation, migration, differentiation of neural stem cells, axonal growth and synapse formation. Other two indexes including trk-B, NOGO-A were used to assess the effects on the neurotropic factor and inhibitory factor.

以&肾生髓&的理论为依据，以MCAO大鼠为实验对象，利用原位杂交技术和免疫组织化学方法，分别以nestin、PSA-NCAM、MAP-2、GAP-43、突触素p38为指标，观察复健片对神经干细胞增殖、迁移、分化、轴突生长、突触形成的影响；分别以trk-B、NOGO-A为指标，观察复健片对神经生长营养因子、抑制因子的影响。

In this experiment, the object was ovaries from adult mouse, we use western blot technique and immuno-histochemistry method to study and research expression and distribution changes of ERβand p-ERβduring oestrous cycle and early gestational period (1~11d).

本实验以成年雌鼠卵巢为实验材料，分别采用western blot技术和免疫组织化学方法，研究ERβ和p-ERβ在小鼠卵巢各发情周期和妊娠早期(1~11d)表达量与定位分布的变化规律。

Methods Male Sprague Dawley rats were induced by ligating the anterior descending coronary artery and were divided into the 2nd, 4th, 8th, 12th and 21st week groups. Elastic fibers were evaluated in the infarct region using Masson trichrome staining, Uana orcein staining and image analysis. The expressions of TNFα and IL-1β were also detected by immunohistochemistry.

SD大鼠冠状动脉前降支结扎形成心肌梗死模型，于术后2、4、8、12、21周时，用Masson染色、Uana地衣红染色技术和免疫组织化学方法，分别观察梗死区弹性纤维和白细胞介素1β(IL-1β)、肿瘤坏死因子的变化，计算机图像分析系统作半定量分析。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达，并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

Osteogenetic role palyed by human foreskin fibrolasts cultured in vitro.

通过组织化学方法，扫描电镜观察证明人皮肤成纤维细胞同家兔皮肤成纤维细胞一样，在体外培养中可以成骨。

Electrochemical oxidization of sulfide on platinum in alkaline solution has been studied by using electrochemical techniques.

用电化学方法研究了碱性溶液中硫化物在Pt电极上的氧化过程。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。