化学方法

We investigated constituent of triterpenoid saponins ofAlbizzia, two new compounds together with two known compounds were isolated from Albizzia julibrissin Durazz. by using column chromatography (macroreticular resin, silica gel, Sephadex gel, reverse phase silica gel),preparative HPLC methods et al.On the basis of spectroscopic analysis, including IR,ESI-MS,~1H-NMR,~(13)C-NMR,HMBC,HMQC,~1H-~1HCOSY and chemical methods, the structure of two new compounds were identified as 3 - O -[β-D-xylopyranosyl(1→2)-β-D-fucopyranosyl (1→6)-β- D -2- deoxy - 2 - acetoamidoglucopyranosyl] -21-O-[(6S)-2- trans- 2,6-dimethyl - 6 - O-β- D - quinovopyranosyl -2,7- octadienoyl] - acacic acid- 28 - O-β-D-glucopyranosyl(1→3)[α-L-arabinofuranosyl(1→4)]-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside acacic acid 3- O -β- D- glucopyranosy(1→3)-β- D- fucopyranosl(1→6) [β-D- xylopyranosyl (1→2)]-β-D-glucopyranoside ;two known compounds were acacic acid lactone 3- O -β-D- xylopyranosyl-(1→2)-β-D-fucopyranosl (1→6)- 2-deoxy -2 -acetoamido -β-D- glucopyranoside ; acacic acid lactone 3- O-β-D-xylopyranosyl(1→2)-α-L- arabinopyranosl (1→6)- 2- deoxy - 2- acetoamido -β-D-glucopyranoside . The study lays chemical foundation and chemical reference substance for enhancing quality standard of Albizzia julibrissin Durazz.

本研究论文在综述国内外对合欢属Albizzia三萜皂苷化学成分和药理作用研究进展的基础上，利用传统植化分离手段和现代分离技术，包括大孔树脂、硅胶、葡聚糖凝胶、反相硅胶等柱色谱，制备高效液相色谱法等技术从中药合欢皮中分离得到了4个化合物，其中，2个新化合物和2个己知化合物，并进一步通过现代分析技术IR，ESI-MS，~1H-NMR，~(13C-NMR，HMBC，HMQC，~1H-~1HCOSY等和化学方法鉴定了2个新化合物的结构分别是：3-O-[β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖基]-21-O-[(6S)-2-反式-2，6-二甲基-6-O-β-D-吡喃鸡纳糖基-2，7-辛二烯酸基]-金合欢酸-28-O-α-L-呋喃阿拉伯糖基(1→4)[β-D-吡喃葡萄糖基(1→3)]-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷，金合欢酸3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃夫糖基(1→6)[β-D-吡喃木糖基(1→2)]-β-D-吡喃葡萄糖苷；2个已知化合物结构分别是：金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-β-D-吡喃夫糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷，金和欢酸内酯3-O-β-D-吡喃木糖基(1→2)-α-L-吡喃阿拉伯糖基(1→6)-β-D-2-去氧-2-乙酰氨基吡喃葡萄糖苷。

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

The rapid development of industry more and more use complex medical methord,so that a significant increase in waste water,and there are many baneful chamicals

快速发展的工业越来越多地采用各种复杂的化学方法，从而使废水大量增多，而很多废水中含有种种有害的化学物质。

Due to more and more complex chemical means adopted in the rapid development industry, the number of waste water which contain variety of baneful chemical substances are increased immensely.

快速发展的工业越来越多地采用各种复杂的化学方法，从而使废水大量增多，而很多废水中含有种种有害的化学物质。

Objective: To extract the volatile oil from rhizome,leaf,flower of chive by chemical method and its chemical components were identified.

目的 ：用化学方法从韭菜的根茎、韭菜叶、韭菜花中提取挥发油，并鉴定出其挥发油的化学成分。

By using freshly isolated SD rat cerebrocortical synaptosomes and KC1 as chemical stimulant to depolarize, we studied the effects of etomidate on KCl-induced increased intrasynaptosomal calcium concentrations, voltage-gated calcium channel subtypes on presynaptic membrane and neurotransmitters release from synaptosomes respectively, via neurochemical and inverse phase high performance liquid chromatographic methods.

我们利用SD大鼠新鲜分离的大脑皮层突触体作为研究对象，以KCl作为化学刺激剂去极化，采用神经化学方法(Fura-2作为钙离子指示剂)和高效液相测定法，分别就依托咪酯对去极化诱发突触体内钙离子浓度升高的影响，突触体膜上电压门控性钙通道亚型的作用以及由此引起的对神经递质释放的影响进行了实验研究。

With electromicroscopic-cytochemical assay, comparative observation on the ultracytochemical localization of ATPase in the mesophyll cells of Euonymus radicans was carried out when the seedlings were cold-acclimated or not and then they were treated at -5℃ for 1d.

采用磷酸铅沉淀的电镜细胞化学方法，对经冷驯化（4℃处理）及未经冷驯化（22℃处理）的金边卫矛以及两者在-5℃下处理一天后的叶肉细胞进行了ATP酶的超微细胞化学定位的比较观察。

The expression pattern of PKC and morphology were investigated during inflammatory pain using immunocytochemistry and histochemistry.

应用组织化学和免疫细胞化学方法观察炎性痛过程中海马神经元形态学变化和PKC活性的改变。

Methods: 11 enzymes and chemicals in human embryonic heart and adult human heart were analyzed adopting histochemistry and hisophotometry.

采用组织化学方法和组织扫描光度测定法，观察胎儿和成人心脏的11种酶和化学物质。

Methods: The expression of VEGF by immunohistochemical staining was examined in 61 cases of the esophageal carcinomas and 13 cases of normal esophageal mucosas. Microvessel density in the cases was determined by FactorⅧ immunohistochemical staining.

应用免疫组织化学方法检测61例食管癌组织及13例正常食管粘膜组织的VEGF表达，并对血管进行Ⅷ因子相关抗原的免疫组织化学染色，计数微血管密度。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失