化学方法

Methods The MMP-3, OPN, and TIMP-1 expressions in LECs of anterior subcapsular cataract (31 cases), nuclear cataract (28 cases) and normal lens (12 cases) were detected by immunohistochemistry, respectively.

采用免疫组织化学方法测定MMP-3，OPN和TIMP-1在31例前囊下性白内障，28例核性白内障和12例正常晶状体上皮细胞中的表达。

Methods After transfection,a stable MRP-overexpressed subline named EJ/MRP was established.Gene expression of MRP and mdr1 were detected by using RT-PCR and immunohistochemistry methods.

将全长MRP cDNA转染膀胱癌EJ细胞，建立稳定表达MRP的亚克隆EJ/MRP，用RT-PCR、免疫组织化学方法检测了MRP和mdr1基因的表达，并检测了其对11种化疗药物的敏感性。

The oxide layer may be chemically growing copper suboxide.

氧化层可以是用化学方法生长的氧化亚铜。

The experimental results of I/V characteristics are measured under various gases and compared with the theoreticalresults predicted by thermionic emission and tunneling model.

用电化学方法测试了其电流/电压在不同气体下的变化曲线，并结合热电子发射理论和电子隧穿理论对实验结果进行了分析。

Author had started the research on design and synthesis of heterocycles several years ago, mainly focused on the design and synthesis of imidazole, thiazole, tetrazole derivatives and their glucosides, in which most of them may have some particular bioactivities.

本论文主要是围绕具有生理活性含氮杂环化合物的设计与合成而展开的，在论文工作中我们应用液相"一锅法"和固相组合化学方法进行了咪唑环、噻唑环、四氮唑环等具有生理活性的杂环化合物及其糖苷的合成。

To avoid the argument, we employed thiophosphate chemistry in the repair patch mapping.

为了避免此项疑虑，因此改用含硫磷酸的化学方法来探讨可能修复路径。

Objective To obtain high titer and specific polyclonal antibody of advanced glycation end products for immunochemical detection of AGEs.

目的制备特异性强、效价高的抗晚期糖化终末产物的多克隆抗体，以用于AGEs免疫化学方法的检测。

In this study, we make a systematic observation on localazation of mast cells in rabbit by toluidine blue staining method.

本研究采用甲苯胺蓝染色法和免疫组织化学方法，对肥大细胞(Mast cell，MC)在家兔体内的分布定位进行了系统观察。

METHODS Using immunohistochemistry method, we observed the Fos expression in the brain of purified protein derivative of tuberculin immunized mice at different time points.

用免疫组织化学方法观察纯化的结核菌素衍生蛋白免疫刺激后不同时间点小鼠脑内的 Fos蛋白表达。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。