化学方法

Methods:The expression of CK20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RTPCR,and the results of CD44v6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry,and then compared with control groups through statistics analysis.

应用荧光定量RTPCR法检测50例大肠癌患者回流门静脉血中CK20mRNA，同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达，并与对照组比较。

Where any doubt arises over the quality of the product, it should not be considered suitable for re-issue or re-use, although basic chemical reprocessing to recover active ingredient may be possible.

若对产品质量有任何疑问，即便通过基本的化学方法返工后可回收有效成份，也不适合于再发货或再使用。

The product is the result of years of research, and is likely to revolutionize all the chemical methods in use at present.

这些产品是我们多年来的研究成果，它将对传统的化学方法产生重大改革。

The product are the result of years of research and are likely to revolutionize all the chemical methods in use at present.

此产品是我们多年来的研究成果它将对传统的化学方法产生重大改革。

In addition,the expression of Galβ-1,4-GlcNAc group was studied by ricinus communis agglutinin-I lectin blot and lectin histochemistry with RCA-I respectively.

并利用特异识别-β1，4-半乳糖苷键的蓖麻凝集素-Ⅰ，经letin blot和组织化学方法分析-β1，4-半乳糖苷键在各型人脑星形胶质细胞瘤中的表达变化和表达定位。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

Objective : To investigate expression of oncogene and its significance in benign and malignant preomorphic adenomas of salivary gland.

采用免疫组织化学方法对5例瘤旁腺体，21例良性多形性腺瘤和24例恶性多形性腺瘤中C-H-ras和P53蛋白产物的表达进行形态学观察。

Methods:Immunohistochemistry and image analysis methods were conducted to examine the expressions of EGF,TNF-α and TGF-β1 in both groups at 3,5,7,9 and 14 days after scalding.

用免疫组织化学方法结合图像数据分析，分别在烫伤后第3、5、7、9、14d检测EGF、TNF-α、TGF-β1表达水平的变化。

METHODS: We performed an inmunohistochemical analysis of 204 SCLC samples to determine KIT protein expression.

本研究使用免疫组织化学方法分析了204例小细胞肺癌组织标本，用来测定KIT蛋白的表达情况。

Ojective To investigate the distribution of H type 1-4 chains of ABO histo-blood group in adult human kidney and their relationships with secretor status.

应用抗ABO抗体及3种糖链特异的单克隆抗H抗体的免疫组织化学方法，检查分泌型与非分泌型个体肾组织中相应抗原物质的分布。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失