化学方法

It is the environmental factors inside and outside pearl mussel that determine the secretion of the nacre.

运用多种组织化学方法和透射电镜技术，研究了5种pH水环境(pH 5、6、7、8、9)对三角帆蚌外套膜珍珠质分泌的影响机制。

The nectary morphology of Sagittaria triforia female flowers were studied using paraffin section and histochemistry methods.

应用石蜡切片和组织化学方法，对慈姑雌花蜜腺的形态结构进行了研究。

Immunocytochemical staining was conducted for the neural stem cells, neuron and neuroglia specific cell surface marker such as nestin, MAP-2, NSE and GFAP.

免疫细胞化学方法检测诱导前后神经前体细胞、神经元和神经胶质细胞特异标记物如nestin，MAP-2，NSE和GFAP的表达。

Abstract］ To investigate the mechanism of reception and transmission of immune molecules in vagus, the expression of IL-1 receptor type Ⅰ in nodose ganglion and vagal paraganglion was studied by using immunohistochemistry.

摘要］ 为研究迷走神经感受和传递免疫信息的机制，用免疫组织化学方法研究了IL-1Ⅰ型受体在正常和免疫激活的大鼠结状神经节和迷走旁节中的表达。

METHODS: The label index of Ki-67 was studied by immunohistochemical method in the tissue of superficial lobe parotidectomy which included the primary and secondary operation cases, nonrecurrent group, and group of low-grade malignant parotid gland tumors.

运用免疫组织化学方法，测定并比较细胞增殖活性相关抗原Ki-67在腮腺多形性腺瘤腮腺浅叶切除术后未复发病例、复发前病例、复发后病例及腮腺低度恶性肿瘤病例术后石蜡标本中的阳性表达率。

All rats were killed 8 days after operation and the expression of transforming growth factor-13 was detected by immunohistochemical method.

术后第8天分别处死各组大鼠，用免疫组织化学方法测定转化生长因子日的表达情况。

Six of rats in each group were sacrificed at days 2, 7, and 14 after operation, and the expression of Fos protein in the spinal cord were assayed with the immunohistochemical methods and image analysis.

于术后第2，7，14天各组分别处死大鼠各6只，取脊髓，应用免疫组织化学方法和图像分析系统检测脊髓中Fos蛋白的表达。

METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

Methods: A early model of brain radiation-induced injuries in the rat was developed. Analysis of the expression of TNF-α TNFR1 and TNFR2 in the pallium was done at different dose and time level after whole-brain irradiation with immunohistochemistry and computer technology.

在大鼠早期脑放射性损伤模型基础上，应用免疫组织化学方法染色标本，运用计算机图像技术计算并分析大鼠大脑放射性损伤后大脑皮质区在不同剂量、不同时间点TNF-α、TNFR1和TNFR2的动态表达。

There were also glands and extracellular materials in different stages of development. Immunocytochemical study showed that factor Ⅷ antigen and PALP were positive in vascular endothelium.

免疫细胞化学方法显示：正常流出或刮出组织的毛细血管内皮因子Ⅷ相关抗原和胎盘碱性磷酸酶呈阳性反应。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失