化学方法

The lesion, the changes of activity of cholinesterase and acid phosphatase of motor neurons in lateral nucleus of spinal cord anterior horn, the function of axoplasm transportation and nerve conduction, the regeneration of axons and myelin sheath, and the recovery of sciatic nerve function were examined at 7, 14, 30 and 90 days intervals after operation, using Nissl and enzyme histochemistry staining, electrophysiological technique, hydrogen-peroxide oxidoreductase retrograde trace method, axon image analysis, and measurement of sciatic function index. The spinal cord anterior horn of injury side was compared to the correspondence region of spinal cord.

分别于术后7、14、30和90d应用酶组织化学方法、电生理方法、HRP （hydrogen-peroxide oxidoreductase）逆行示踪方法、轴突图象分析方法以及坐骨神经功能指数（sciatic nerve function index，SFI）测量等方法检测坐骨神经损伤后对应脊髓神经元的存活率、神经元胞体酶系变化、损伤神经在轴浆运输、电传导以及轴突、髓鞘再生等方面的恢复情况，探索外周神经损伤后OECs及几丁质对神经元的保护作用以及对新生神经功能恢复的作用，为外周神经损伤的治疗提供新的理论基础。

The lignification of bamboo stem were studied by means of light microscope and Transmission electron microscope coupled with histochemical and cytochemical methods.

应用光学显微镜和透射电子显微镜，并结合组织化学和细胞化学方法，研究了毛竹茎各组织中细胞壁的木质化过程、木质素异质性、酚酸类成分的分布、木质素在细胞壁中的沉积方式以及过氧化物酶的组织、细胞化学定位等。

Methods: cell biological assay, histochemical staining of senescence associated-β-galactosidase and immunohistochemical staining of proliferating cell nuclear antigen wereused. Results: chondrocytes exhibited various types of amitotic figures.

应用细胞生物学方法、衰老相关-β-半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法，研究正常成人和正常成体大鼠气管软骨组织动力学。

Currently, the main means of pre -desilicification for bauxite involve chemical methods, biological ways and physical methods.

目前铝土矿预脱硅的主要方法有化学方法、生物方法和物理方法。

The corrosion inhibition of isoquinoline and its hyd roxy and carboxylic derivates were determined by electrochemical methods,and qua ntum chemistry calculation were gained through HMO and CNDO.

用电化学方法实测了异喹啉及羟基、羧基两类取代衍生物的缓蚀效率，并用HMO、CNDO方法进行量子化学计算。

Nowadays, there are mainly three kinds of methods for consolidating excavated silk fabrics.

目前常用的加固方法主要有三大类：物理方法、化学方法和生物方法。

The chemical method which is fit for tube and bar products has been the basis for forming a series of GB, but it needs subjoining.

其中适用于管棒材的化学方法已形成系列国标，但仍需增补；适用于板带材的化学方法仅限于试验研究之用。

Methods Healthy Sprague-Dawley rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer.

采用腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注的方法处理SD大鼠，并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。

objective:to investigate the expression and significance of rb in the occurrence, development and regression of infantile hemangiomas.methods:the expression of rb was examined in the proliferative stage and catagen of human hemangiomas and normal skin tissues by using immunohistochemical technique.immunohistochemical technique for factorⅷ-related antigen was used to prove that the cells which expressed rb were endothelium.image analysis system was applied to measure the expression level of rb at different stages of hemangiomas and in normal skin tissues.results:the expression of rb was significantly lower in proliferating hemangiomas than that in involuting hemangomas(p.05).conclusion:rb might play an important role in the regression of human hemangioma endothelial cells and anti-angiogenesis.

目的：探讨rb蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法：采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中rb的表达水平，并结合第ⅷ因子相关抗原的免疫组织化学染色证实表达rb的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织rb表达的积分光密度和面积。结果：增生期血管瘤内皮细胞rb表达水平低于退化期，差异有显著性（p.05），退化期血管瘤内皮细胞rb表达水平与正常皮肤组织相比，差异有显著性（p.05）。结论：rb通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。

Physical or chemical methods for detoxification of DON resulted in losses of nutrients, incomplete detoxification of mycotoxin and the residue of chemical components.

常规的物理或化学方法脱毒方法会破坏谷物的营养成分，导致脱毒不完全或残留化学成分。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失