化学方法

Well dispersed platinum nanoparticles and those with special shapes were synthesized through electrochemical and thermochemical methods.

采用电化学方法、热化学方法在水相中制备了分散性良好的铂纳米粒子以及具有一定形貌的铂纳米粒子结构。

Methods The expression of HIF-1α was assayed by means of immunohistochemical technique in 60 patients.

方法通过免疫组织化学方法，了解HIF-1α在60例原发性颊黏膜鳞癌中的表达，分析其阳性表达水平与颊癌临床分期、病理分级以及预后等临床病理因素之间的关系。

Methods: immunohistochemical assay was used to detect cox 2 in 29 cervical cancer, 14 chronic cervitis and 18 normal cervixes. immunohistochemistry and in situ hybridization were used to detect the cox 2 protein and mrna in cervical cancer cell lines: siha and hela. the relationship between the expression of cox 2 and the clinicopathologic parameters of cervical cancer was analyzed.

用免疫组化方法检测29例宫颈癌患者cox 2的表达水平，并以14例慢性宫颈炎症患者和18例正常宫颈上皮为对照组；用免疫细胞化学方法和原位分子杂交法检测宫颈癌细胞株siha和hela中cox 2蛋白及基因表达。

Material and Method: 40 paraffins of prostate carcinoma from 1996 to 2005 were collected.

材料和方法：搜集昆明医学院附属第一院和第三院1996年～2005年间临床资料完整的40例未经治疗前列腺癌患者的前列腺癌组织标本，应用免疫组织化学方法检测雄激素受体的表达。40例病例根据肿瘤的组织学形态进行病理分级。40例病例术前均行了盆腔前列腺CT扫描。

Most 5-HT cells appeared in the base part of gland in the rat, while in the human they were mainly located in the intercellular substance and a few scatted in the glandular epithelium.

方法采用免疫细胞化学方法，检测人和大鼠胃窦部粘膜内生长抑素细胞、胃泌素细胞、5-羟色胺细胞（5-HT细胞）、嗜铬粒素A细胞的分布。

Objective To study the effects of berbamine and batiolun on LDH metabolizability in the process of increasing leukocyte.Methods Enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of LDH metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内LDH的代谢的影响方法采用酶细胞化学方法，对两药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时，对乳酸脱氢酶活性的影响进行了比较观察。

objective to study the effects of berbamine and batiolun on ldh metabolizability in the process of increasing leukocyte.methods enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of ldh metabolizability.results when leukocyte is inhibited by the immune inhibitor cycolophosphamcide,berbamine and batiolun are compared of the effect of ldh activity.conclusionthe study draws the conclusion that both berbamine and batiolun can increase thegranule cell,and they also have some effect of the ldh metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内ldh的代谢的影响。方法采用酶细胞化学方法，对两种药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时，对乳酸脱氢酶活性的影响进行了比较观察。结果小檗胺和鲨肝醇均可升高粒细胞，同时对细胞内ldh的代谢有一定的影响。结论小檗胺和鲨肝醇能使外周血粒细胞增加，可用于治疗多种因素引起的白细胞减少症。

After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法，并以0.25%胰蛋白酶消化，以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体)，以庆大霉素对培养的肾小管上皮细胞建立损害作用模型，然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

In this paper, the studied results showed that the key of plate wastewater treatment by chemical method was proper value of pH, and the electrolysis method was applied to treat mixed wastewater of chromium and other heavy metals.

通过研究得出，利用化学方法处理电镀废水的关键是控制好pH值；另一点是采用微电解方法处理含铬和其它重金属混合废水，在实际工程运作中有良好的效果。

Methods The SD experimental rats were divided into four ovarian stages basing on the vaginal lavages, from which the ovaries were obtained in every cycling stage.

利用阴道涂片的方法将实验大鼠分为四组不同的卵巢周期，采集不同时期卵巢标本，采用免疫组织化学方法观察卵巢中NR4A1蛋白的定位及表达情况。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失