化学合成

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应，本文采用化学和分子生物学相结合的方法，研究了不同浓度乙烯利处理玉米后，叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化，典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化，以及其他调控次生代谢物的关键基因表达的时间动态。

The advantages of α-hydroxyacetic acid biosynthesis over traditional chemical conversion are wide range of reaction condition,high conversion ratio and green process.

化学法生产羟基乙酸存在原料消耗大、产品收率低、环境污染严重等缺点；生物法合成羟基乙酸条件温和、底物转化率高，为绿色合成羟基乙酸提供强有力的技术支持。

The research of indoles has been one of the most active areas of heterocyclic chemistry. In particular, 3-substitued indole derivatives have been received much attention as building blocks for the synthesis of many natural products and other biologically active compounds.

目前吲哚化学的研究是杂环化学中最活跃的领域之一，特别是3-取代吲哚衍生物，已用于许多天然产物和相应具有生物活性化合物重要骨架的构筑，其合成方法的研究格外令人注目。

The iodometric titration was used to determine the oxida- tion state of Fe ions and the oxygen nonstoichiometry in La1-xSrxFeO3-δ.

用FTIR、TG-DTA、XRD等对产物形成过程及结晶学特征进行了研究，考察了合成样品与Sm0.2Ce0.8O1.9的化学相容性；利用碘滴定法测定了该系列产物中Fe元素的平均表观化合价和氧的非化学计量值。

Based on the concepts of fragrance chemistry, under the guidance of molecules structure and odour relationships, we synthesized a list of potential odorants 46a-s, 47a-b, 48a-d and 49a-e by esterification, lactonization and reduction of the Ireland-Claisen rearranged products. Some of them were evaluated.

四、基于合成香料化学目前发展的现状，以香料的分子结构对香气影响的一些规律为指导，对所获得的部分Ireland-Claisen重排产物羧酸进行结构修饰，合成了系列潜香分子46a-s，47a-b，48a-d及49a-e，并进行了评香。

By the methods of molecular biology, enzymology, chemistry, physics the project was carried out. The genes encoding leucyl- and arginyl-tRNA synthetase , and tRNA and tRNA from Escherichia coli were cloned and overexpressed in E.

以大肠杆菌亮氨酰tRNA合成酶和精氨酰tRNA合成酶为对象用分子生物学、酶学、酶动力学、化学、物理学的方法进行该项研究。

In this project, a series of novel polypyridine ligands and their ruthenium complexes were synthesized. The scope of this work is focused on the construction of multinuclear complexes, the controlling of electron and energy transfer in polynuclear complexes, NLO materials and molecular recognition, The results should be of value in further understanding the properties of the Ru complex, as well as laying the foundation for the rational design of Molecular devices based on Ru complexes.

本课题的主要目的是应用化学手段，设计合成出一系列结构新颖的多吡啶配体及其钌配合物，在多核配合物的构筑、电子和能量转移调控、非线性光学材料和分子识别等方面进行重点研究，旨在进一步丰富和发展钌多吡啶配合物化学。

In summary, a low-cost but high-power microwave chemical reactor with double-magnetron based on noncoherent power synthesis is researched.

以上结果说明基于微波非相干功率合成技术的低成本大功率微波化学反应器的设计方法是合理的，这为低成本的大功率微波化学反应器的设计提供了重要的理论依据，对微波应用在化工领域的推广具有重要的现实意义。

The synthesis process of peracetic acid and its industrial application in environment,energy engineering,polymer/resin industry and biomimetic chemistry are introduced.

介绍了过氧乙酸合成工艺及在环境、能源、聚合物/树脂和仿生化学领域的最新应用研究进展。分析了合成工艺的缺陷并提出了工艺改进的建议。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失