化

The results showed with the fruit ripening granulation index rose; semicellulose content of granulated juicy sac decreased first and then increased, while semicellulose content of non-granulated juicy sac reduced; the cellulose content of granulated juicy sac rose, while cellulose content of non-granulated juicy sac decreased first, and then rose to the top, and then reduced; and protopectin content reduced.

结果表明：粒化指数随着果实成熟而上升；粒化汁胞的半纤维素含量下降后上升，未粒化汁胞的半纤维素含量下降；粒化汁胞的纤维素含量增加，未粒化汁胞的纤维素含量下降后上升又下降；原果胶含量降低。

Restoration of ODC expression in the HL60-ODC cells resulted in rescue of rottlerin-induced apoptosis of HL60 cells. Simultaneously, phosphothreonine-ODC of rottlerin-treated HL60-ODC cells was highly elevated comparing with HL60-pcDNA3 cells. Taken together, we suggested that rottlerin, a PKCd inhibitor, might decrease phosphothreonine-ODC to reduce both protein stability and enzyme activity of ornithine decarbooxylase and finally resulted in cell apoptosis.

为了侦测鸟胺酸去羧化是否能够负调节rottlerin所引发的细胞凋亡，我们将具有鸟胺酸去羧化cDNA载体稳定转染至HL60细胞内，此细胞称为HL60-ODC细胞，另转染载体的细胞称为HL60-pcDNA3细胞；实验发现HL60-ODC细胞中的鸟胺酸去羧化蛋白为HL60-pcDNA3细胞与HL60细胞表现之2.5倍，同时HL60-ODC细胞提升鸟胺酸去羧化蛋白表现，能够减少rottlerin造成之细胞凋亡现象，并且此蛋白在HL60-ODC细胞中酥胺酸磷酸化较HL60-pcDNA3细胞表现高。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

Three in vitro gas production experiments were conducted using the procedure described by Menke et al.(1979) to investigate the effect of steam conditioning time, processing method and corn variety on the gelatinization of starch and ruminal fermentation characteristics of corn grains.

本文应用人工瘤胃法，进行以下三个试验研究：1 不同调制时间预处理的蒸汽压片高油玉米对淀粉糊化度和人工瘤胃发酵的影响；2 比较最佳调制时间处理的蒸汽压片、微化、膨化及爆破不同方法处理的高油玉米对淀粉糊化度和人工瘤胃发酵的影响；3 不同品种玉米对淀粉糊化度和人工瘤胃发酵的影响。

The results show that the SAPO–5 has a pillar-like morphology with the size of about 20 μm×5 μm×5 μm.The ratio of P:Al:Si is 1:1.19:0.67 in its crystal structure, the content of Si is high. The total acidimety fo the SAPO–5 is 578.0 μmol/g , hihger than that of prepared by one step crystalline hydrothermal method.And its initial catalysis character performance on normal butane isomerization reaction reach 24.8 %, higher than that of prepared by one step crystalline hydrothermal method.

实验结果表明：产物为柱状的磷酸硅铝分子筛，其晶粒尺寸大约为20 μm×5 μm×5 μm，其晶体结构中P：Al：Si＝1：1.19：0.67，硅含量较高；采用分段晶化方式制备的SAPO-5分子筛的总酸量为578.0 μmol/g高于一段晶化方式制备的SAPO-5分子筛的总酸量；采用分段晶化生成的SAPO-5分子筛的催化正丁烷异构化反应初始活性可达到24.8 %，远高于一段晶化制备的SAPO-5分子筛的初始活性14.1 %。

BMP-7 expression was lower markly in Lung-kidney Qi Deficiency 、Liver-kidney yin Deficiency、 Qi-yin asthenia、Speen –kidney Yang Deficiency than normal control. BMP-7 expression decreased with Lung-kidney Qi Deficiency 、Liver-kidney yin Deficiency、 Qi-yin asthenia、Speen –kidney Yang Deficiency.It shows that the level of BMP-7 expression may play a role in Zheng differentiation-classification of TCM of the chronic glomerulonephritis.

中医证型与肾间质纤维化程度显著相关，肺肾气虚、肝肾阴虚型纤维化程度最轻，气阴两虚型其次，脾肾阳虚型纤维化程度最重，提示传统中医辩证分型与纤维化程度有关，在一定程度上可以反映慢性肾小球肾炎组织学损伤程度，肾间质纤维化程度可作为中医临床辨证参考依据。

A sulfonated poly aryl ether with sulfonic group on naphthalene ring of side chain and preparation thereof, which is capable to solve technical problems of complexity in synthesis, hardness in controlling sulphonation position and degree, high possibility of side reaction in sulphonation, complexity in sulphonation and hardness to realize indutrialzation.

一种磺酸基在侧链萘环上的磺化聚芳醚及其制备方法，解决现有技术中合成方法复杂，磺化位置和磺化度难以控制，磺化过程中容易发生多种副反应，磺化工艺复杂难以实现工业化等技术问题。

Also, the type of dispersion of four gasliquid-liquid systems: air-decane-aqueous solution of sodium sulfite and surfactant, airmixture of decane and dodecene-aqueous solution of rhodium complex, ligand, and surfactant, air-mixture of decane, dodecene and tridecyl aldehyde-aqueous solution of rhodium complex, ligand and surfactant, and air-mixture of decane and dodeceneaqueous solution of trisodium salt of tri (m-sulfophenyl) phosphine, and surfactant is determined by electric conductivity.

本文采用电导率测量的方法实验研究了空气—正癸烷—亚硫酸钠和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷、1-十二碳烯和十三醛混合物—铑膦络合物催化剂和十六烷基三甲基溴化铵水溶液、空气—正癸烷和1-十二碳烯混合物—三间磺化苯基膦三钠盐和十六烷基三甲基溴化铵水溶液四个三相体系的液液分散特性。当有机相与水相体积比分别为7／3、1／1和3／7时，前三个体系均形成水包油型分散，并有稳定的乳状液生成。

In chapter 4, we have described an improved method for the preparation of ethynylferrocene. Ethynylferrocene was prepared in satisfactory yield by reaction of formylferrocene with triphenylphosphine and carbon tetrachloride followed by dehydrohalogenation in the presence of n-BuLi.

第三章中，我们研究了钯催化下碘杂环化合物的羰基化—酰胺化反应、羰基化—酯化反应和与末端炔烃的羰基化交叉偶联反应，为一步合成多官能团化合物开辟了新的途径，同时，得到的这些产物经过进一步官能团转化后，可以成为一些天然产物合成中的重要中间体化合物。

Firstly, by analyzing existing models of predicting concrete carbonation and its influencing factors, a practical model of predicting the depth of carbonated concrete is proposed in this thesis, which takes the compressive strength of concrete as the main parameter and considers influence of temperature and moisture of environment, construction factors, stress condition and density of CO2 in the environment, and plenty of spot-data has been used to verify the model; By means of regression analysis of the spot-data, the calculating model of uncarbonated remainder is formulated; Considering the randomness of concrete carbonation, the paper put forward a probability analysis method of concrete carbonation span-life.

首先，论文在分析已有混凝土碳化预测模型与影响因素的基础上，建立了以混凝土抗压强度为参数，考虑环境温湿度，施工因素，应力状态及环境CO_2浓度影响的混凝土碳化深度实用模型，并利用大量工程实测数据对模型进行了验证；通过对实际工程检测数据的回归分析，给出了碳化残量的计算公式；考虑到混凝土碳化的随机性，提出了混凝土碳化寿命的概率分析方法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。