包膜

In addition, HCC tissues with peplos encroachment showed lower expression rates than others without peplos encroachment (P＜0.05). There was no other significant correlations of Plk1 expression with either age, tumor diameter and number, lymphoid node metastasis, extrahepatic metastasis, or portal vein embolus could be established.

高分化肝癌中Plk1蛋白的表达明显高于中低分化肝癌(P＜0.01)；无包膜侵犯的肝癌组织中Plk1蛋白的表达高于有包膜侵犯的肝癌组织(P＜0.05)；Plk1的表达与患者年龄、肿瘤直径、肿瘤数目、淋巴结转移、肝外转移、门静脉有无癌栓无关。

Viral infection of the host cell may be switched on by the specific and stable combination of one or several viral envelope protein with one or several glycoprotein receptors available on the cell membrane, which is followed by viral pinocytosis or viral fusion with cell membrance, viral deenvelope, viral particle movement from cytoplasm towards nucleus and the viral gene passage.

病毒感染细胞可能是通过一个或几个病毒包膜蛋白与一个或多个细胞膜上的糖蛋白受体特异稳定地结合而启动，通过病毒胞饮作用或者病毒与胞膜融合、病毒脱包膜、病毒粒子从胞浆到胞核的运动和病毒基因通过核膜的传代将会发生；但参与这些动力过程相关的病毒、细胞结构和生化因子仍不清楚。

The use of lemon acid could stimulate the root activity of potherb mustard significance, and the use of lemon acid and phosphate could maintain the output high and reduce heavy metals of plants.

包膜柠檬酸处理对雪里蕻根系活力的提高作用最为显著，包膜柠檬酸和磷肥在提高产量的同时也显著降低植株对重金属的吸收。

Results 10 cases of single tumor,2 cases of double tumors in one kidney; one of them is mergering subcapsular hematoma,and the other is mergering subcapsular and retroperitoneal.

结果：单发肿瘤10例，单侧肾脏2个肿瘤2例，合并肾包膜下血肿4例，合并肾包膜下及腹膜后血肿1例。ct表现为低、等、高混杂密度病灶，增强扫描低密度、等密度病灶可有不同程度的增强，高密度病灶不增强。

A series of coated urea and coated compound fertilizer were prepared with rosin and Tung oil as film-forming materials.

以桐油为主要成膜材料制备了桐油包膜尿素和桐油包膜复合肥料。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Porcine hemoglobin polymer is made in imitation as the substtitute for blood product. Pseudorabies virus is chosen as the enveloped DNA model virus and the Porcine Parvovirus is chosen as the nonenveloped DNA model virus. Complete DNA sequences of these two kinds of virus are analyzed and specific fragments are selected as examinable targets. Establish a new method based on real time PCR combined with cell infection assay to evaluate virus clearance efficiency in blood product.A new method is established to remove the virus from blood product.

本研究以戊二醛聚合猪血红蛋白模拟血液制品，选取PRV、PPV分别作为血液制品中有包膜DNA指示病毒和无包膜DNA指示病毒，针对特定两种指示病毒PRV、PPV，进行了生物信息学分析，选取特定片段作为扩增靶点进行检测，建立了一种基于实时荧光定量PCR技术的检测方法，联合病毒的细胞感染力实验，用以评价血液制品中指示病毒的灭活去除效率。

Application performance of the end products is tested,and it is found that both the dispersion properties and decentralized stabilization of the products treated with the oleiferous agent have been increased,while being inconspicuous with the aqueous agents.

试验分别用水性分散剂和油性分散剂对钛白粉半成品进行粉碎包膜，检测成品的应用性能，发现水性分散剂效果不明显，而油性分散剂对分散性和分散稳定化程度都有提高，同时分析了在锐态型钛白粉行业产品包膜效果的影响因素，以供参考。

A central venous catheter was inserted for hemodynamic monitoring and blood transfusion. Cardiac tamponade occurred secondary to perforation of the superior vena cava by the central venous catheter. The patient's hemodynamic status became stable after pericardiocentesis. One week later she was discharged with no sequelae during the three month follow-up.

患者术后装置中央静脉导管以利於血行动力的评估及输血，病患进行中央静脉导管术时因上腔静脉穿刺破裂导致心包膜填塞及休克，经紧急照会心脏科医师后立即给予心包膜穿刺术，术后患者血压渐趋稳定情况好转於一星期后出院，经过三个月的追踪亦无后遗症。

Results 12 cases, there were 12 isolated subperiosteal hematoma of the skull, all in the top three weeks ~ March 2 cases, six months in March ~ 9 cases, one cases of more than half a year. 3 weeks to 3 months of hematoma, capsule machine was of uneven thickness of the line, a higher density strips; March ~ subcapsular hematoma of more than half a year with irregular thickening of the skull-shaped double-decker was changed after Hematoma shaping a longer period of time to become part of skull.

结果：12例中，共存在12个孤立的颅骨骨膜下血肿，全部位于顶部，3周~3月2例，3月~半年9例，半年以上1例。3周~3月的血肿，包膜机化呈厚薄不均的线状、条带状更高密度影； 3月~半年以上的血肿包膜不规则增厚与颅骨呈双层状改变，经过更长时间的塑形血肿成为颅骨的一部分结论： CT能准确诊断颅骨骨膜下血肿机化，并充分显示其大小、部位。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。