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After calculating the concentration by counting board, cell suspension was cultured on the coverslips coated poly-L-lysine at 1×109/L, then the coverslips were paved in the bottom of 35 mm culture dish for 3 weeks.

经12 h+ 24 h两次差速培养后,吸出细胞上悬液,计数板计算浓度后,按1×109 L-1浓度重新种植于载有多聚赖氨酸包被盖玻片的35 mm培养皿中继续培养3周。

Among it wrap up surface auxiliary that cover,-COOH,-OH, sulphur base S032~ sour hydroxyl carboxyl , etc. group last the not facilitating last aluminium the buoyancy of floatings of the powders, and C-C chain , alkyl phenyl R-C6HU fatty alcohol base fatty acid base R-COO, R-0- long in structure, etc. base group kiss not oily, base these group add , facilitate aluminium powder water build rise of area.

表面包覆的助剂中,羧基-COOH、羟基-OH、磺酸基SO_3~(2-)等集团是亲水性的有助于提高铝粉的漂浮力,而C-C结构长链、烷基苯基R-C_6H_(14)脂肪醇基R-O-、脂肪酸基R-COO~-等基团是亲油性的,这些基团的加入有助于铝粉水盖面积的提高。

Zak mixed 840 ml of vermiculite with 60 ml of finely ground peat moss in the flask, moistened this substrate with 550 ml of MMN nutrient solution, capped the flask with an inverted glass having a cheesecloth-wrapped cotton plug cemented to the bottom, and autoclaved the assembly for 45-60 min at 120℃.

萨克氏混合840 ml的蛭石与60 ml磨细的泥碳土於烧瓶中作为基质,以550 ml的MMN营养液润湿之,再以纱布包棉花塞黏於玻璃杯倒盖之,并将整组器具於120℃高温高压灭菌45-60分钟。

Synthesis apparatus Zak mixed 840 ml of vermiculite with 60 ml of finely ground peat moss in the flask, moistened this substrate with 550 ml of MMN nutrient solution, capped the flask with an inverted glass having a cheesecloth-wrapped cotton plug cemented to the bottom, and autoclaved the assembly for 45-60 min at 120℃.

萨克氏混合840 ml的蛭石与60 ml磨细的泥碳土於烧瓶中作为基质,以550 ml的MMN营养液润湿之,再以纱布包棉花塞黏於玻璃杯倒盖之,并将整组器具於120℃高温高压灭菌45-60分钟。

Methods The retinal of 20 post-natal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion.The cell suspension was implated in 6-well culture plates covered with hyaluronic acid and laminin in each well.

用胰酶消化法将20只出生2~3 d的SD(Sprague-Dawley)乳鼠视网膜制成细胞悬液,接种于经多聚鸟氨酸和层粘连蛋白包被的血盖片中。

I loved the part in the car when Min-woo was sleeping, and Kae- Hwa covered his head with the cloth she used to wrap the kimbap.

谢谢摘要!我喜欢民宇在车里睡觉的部分,开花用包紫菜包饭的布盖住民宇的头。

Wrap your mattress, pillows and box springs in zippered covers that area dust proof and allergen proof.

包你的床垫,枕头及信箱泉水拉链复盖面积,防尘及过敏证明。

After the drum on the treatment is the key to a new after the paper in a zhangba unwatering of copy paper, print this screen you will pass a carbon into drum in a white, then remove the copy paper, so that the screen is clearly see what parts should be single pad and which parts do not pad, and which part should be dredged, dredged area must have a slope should be completed on the knife, which lost network and wrapped a drum after proofing effect, when you are satisfied you can open mechanism cards and membership cards.

版上好后滚筒的办理不离是盖脚换上陈的滚筒纸后本质不收一张八开的复写纸,不收空印一次这样网版不离会堵住复写纸印到滚筒边的白纸上,然后把复写纸掏出,这样网版不离澄辉不天看出哪些部位不该独垫,哪些部位不垫,哪些部位不该挖去,挖去的部位必定用不该小刀修成有坡度,这样不会失网点,包好滚筒后打样一张看成果,如果不满即可开机制卡和会员卡制作。

Geiger's own GT body kit was used to give the car a lower, meaner look and to let the turbos breathe easier without destroying the Corvette's brawny lines.

盖自己的燃气轮机身体包被用来给予较低的车,外观和灵活的涡轮增压引擎,让呼吸更容易不摧毁克尔维特的粗壮线条。

Thanks to the practical lid, the package is resealable so that the product remains in the package throughout the entire consumption cycle.

多亏了实际盖,是可重复密封包装,使产品仍然在包在整个消费周期。

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