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Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达:取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例,其中骨旁骨肉瘤4例,纤维母细胞型骨肉瘤13例,软骨母细胞型骨肉瘤12例,骨母细胞型骨肉瘤26例,切成3μm的组织切片备用。

MATERIAL AND METHODS: By kept in archives 53 OS and osteochondroma specimens embedded in paraffine were chosen.

材料与方法:本实验选择存档中石蜡包埋通常型骨肉瘤标本53例,骨软骨瘤标本 20 例。

In diagnosis of aplastic anemia, anemia, lymphoma, osteofibroma, MDS and myeloproliferative disease, bone marrow biopsy is better than puncture biopsy.

骨髓塑料包埋切片与涂片检查起互补作用,诊断血液病须把两者结合起来分析,才能提高诊断的准确率。

RT-PCR detection of SYT-SSX mRNA can be used in formalin-fixed, paraffin-embedded tissues as a sensitive and specific technique in the diagnosis and differential diagnosis of synovial sarcoma. 2. The SYT-SSX fusion type is related to histological type and cell proliferative activity of synovial sarcoma. 3. Some characteristic sequence motifs flank genomic breakpoints of chromosomal translocation of synovial sarcoma, including Alu sequences, Translin recognition sequences, topoisomerase Ⅱ recognition sites and palindromic sequences, which may be associated with chromosomal translocation of synovial sarcoma.

1、RT-PCR技术检测SYT-SSX mRNA表达可用于福尔马林固定、石蜡包埋组织,敏感性和特异性高,可用于滑膜肉瘤诊断和鉴别诊断。2、SYT-SSX融合基因类型与滑膜肉瘤组织学分型及细胞增殖活性相关。3、滑膜肉瘤染色体易位断裂点旁存在一些特征性序列,包括Alu序列、易位素识别序列、拓扑异构酶Ⅱ识别位点、回文序列等,可能与其染色体易位发生机制有关。

From two elevation altitude habitats of 3600 m, 2500 m at cold regions were analyzed and compared by means of light and scanning electron microscope using a new embedding method with PEG and Epon-812. Our results indicated that Papaver croceum Ldb.

采自乌鲁木齐河源区海拔3600m、2500m两种海拔高度,采用PEG和Epon-812两次包埋方法,在光学显微镜和扫描电子显微镜下叶片剖面结构进行了观察。

Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.

将其植入恒河猴背阔肌的肌袋内,以未转染GFP的BMSC用同样的方法接种块状可吸收HA上,作为对照,术后6周取材,4%的中性多聚甲醛固定,塑料包埋,制作骨磨片PI染色在激光共聚焦显微镜下进行观测。

Methods DNA was ext racted from paraffin-embedded tissue blocks of 44 angioimmunoblastic lymphaden o pathy patients and analyzed with polymerase chain reaction for IgH and TCRγ gene rearrangement. Immunohistochemistry staining was used to detect p53 protein expression.

方法运用聚合酶链反应技术检测44份AIL石蜡包埋组织标本中IgH及TCR γ基因重排情况,采用免疫组织化学ABC法染色技术检测AIL免疫表型及p53蛋白表达。

The bone marrow hyperplasia degree, the proliferation and infiltration of plasmocyte, and the reticulin integral were compared respectively in the plastic embedded bone marrow sections and the bone marrow smears of 38 cases of MM, 20 cases of reactive plasmacytosis and 20 cases of normal control group.

同步观察多发性骨髓瘤38例患者骨髓涂片和活检塑料包埋切片。

The distribution of γ-aminobutyric acid and serotonin (5-HT) immunoreactive neurons in the protocerebrum and deutocerebrum of the butterfly Minathyma schrenckii were studied with the Colophony-paraffin embedding serial section technique and the Streptavidin-peroxidase immunohistochemical method.

采用树脂石蜡(Colophony-Paraffin,CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase,SP)免疫组织化学方法,首次研究了 GABA 和5-HT 免疫阳性神经元在白斑迷蛱蝶前脑和中脑中的分布。

Salmonella pullorum antibody was successfully modified on the platinum electrode surface to fabricate the label-free immunosensor by embed technology with gelatin as matrix.

以明胶作为载体,采用包埋技术成功地将鸡白痢抗体修饰于电极表面,制成非标记型鸡白痢免疫传感器。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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