包埋

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Methods Coixendide was extracted by acetone from coix;Gelatin was selected as the wallmaterial and coixendide was microcapsulated by the method of oil-phase drying.

目的探讨油相干燥法制备薏苡仁酯微胶囊化的最佳工艺条件，得出包埋率高、感官质量好的薏苡仁酯微胶囊产品。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

After fixation,the brain was cut coronally into four blockes:the middle partof cerebrum,midbrain,pons and medulla oblongata.These blockes were embeddedroutingly and serial 8μm sections were prepared for HE,specific histochemical andimmunohistochemical stainings.

所有脑组织经固定后冠状切取大脑中部、中脑、桥脑及延髓4个组织块，常规包埋，连续切片（8μm）分别作HE、组化及免疫组化染色。

Materials and MethodsMaterials; 10% formalin fixed, paraffin -embedded tissue samples, including ; MF 59 cases, neurodermatitis 20 cases, chronic eczema 20 cases, parap-soriasis 40 cases, all taken from laboratory of dermatopathology in No.

材料和方法实验材料：来自中国医科大学附属第一床医院皮肤科病理室，10%福尔马林固定、石蜡包埋组织标本：蕈样肉芽肿59例、神经性皮炎20例、慢性湿疹20例、副银屑病40例、临床及病理都符合其疾病的诊断标准；正常皮肤组织标本20例：来自中国医科大学附属第一医院整形外科手术切除的正常人正常皮肤。

In order to simplify the procedure and obtain high quality gDNA, we alternate water-bath to dewax, extract gDNA from hepatocellular carcinoma embedded by paraffin, and amplify gene using HCC gDNA.

为了简化操作，获得更高质量的gDNA，我们采用水浴法替代二甲苯进行脱蜡，亲和层析纯化石蜡包埋肝细胞癌组织标本中基因组DNA，以纯化gDNA为模板PCR扩增看家基因。

We used phospholipid, Epikuron 200, and cholesterol as material to prepare liposome formulation, and discuss the effect of liposome formulation which contain phospholipid and cholesterol or the mix of ionic surfactants such as stearylamine, dicetyl phosphate and nonionic surfactant such as Tween 80. For the three group that physical properties of drug entrapment efficiency, particle size and stability of formulation on day 0, 14, 21 and 30 were respectively compared, and further, we were sieve out optimization of formulation.

我们利用Epikuron 200之磷脂质，以及胆固醇作为制备微脂粒处方之材料，并探讨处方不加或分别加入表面修饰剂stearylaminem与dicetyl phosphate以及非离子界面活性剂Tween 80对处方之影响，且比较此三组微脂粒的物理性质，包含包埋率、粒径大小以及处方在第0、14、21、30天之安定性的变化，进一步筛选出理想之处方。

The results showed that MGM had good spherical shape and narrow size distribution,and the particles with diameters ranging from 0.13 mm to 0.28 mm were about 91 wt%.The surface hydrophilicity of Fe3O4 microcrystal was improved by formamide dispersing treatment.The gel particles made by the copolymerization of glycidyl methacrylate and N,N′-methylene bisacrylamide could encapsule Fe3O4 microcrystal to produce colloid cores,and uniform and stable MGM microspheres were formed by the conglomeration of the colloid cores.

结果表明，合成的MGM呈球形，且粒度分布较窄，粒径为0.13～0.28 mm的粒子占91%；甲酰胺分散Fe3O4，微晶表面的亲水性进一步增强，单体甲基丙烯酸缩水甘油酯和 N，N′亚甲基双丙烯酰胺交联共聚生成的胶粒能够包埋Fe3O4微晶形成胶核，胶核聚集形成均匀、稳定的MGM微球。

Objective using abdominal ligation and fallotomy of oviduct to obstruct fallopian tube Methods Madlener's method of tubo-sterilization and Pomeroy's method of tubo-sterilization is called tradition method,Iring method of tubo-sterilization and modified Pomeroy's method of tubo-sterilization is called modification method.

目的 采用经腹输卵管结扎、切断术阻塞输卵管通道。方法将输卵管卷曲结扎术、输卵管卷曲结扎切断术称为传统法即A组；输卵管抽蕊包埋术、改良输卵管卷曲结扎切断术称为改良法即B组。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。