包埋

METHODS: Fresh bovine tendo calcaneus collagen was dispersed in 0.5 mol/L acetic acid, co-precipitated with chitosan and lyophilized. Dry membranes were cross-linked in 0.05% glutaraldehyde for 24 hours. In vitro its degrading rate was measured by use of collagenase degrading test. The chitosan-collagen membrane was implanted to subcutaneous dorsal sites of SD rats. After implantation, histocompatibility, vascularity and degradation were observed in vivo.

以酸溶法制备胶原溶液，添加一定比例的脱乙酰化几丁质，冻干成膜，0 。0 5 %戊二醛溶液浸泡交联，行体外酶降解实验，检测其耐受酶降解能力；将制备的几丁质-胶原蛋白膜包埋于 36只 SD大鼠皮下，术后 3、5天，1、2、3、4、6、8和 12周取材，检测其组织相容性、血管化能力及材料在体内降解情况。

Thoracic closed drainage, pleuroclysis, gluing through fibrobronchoscopy, fistula mending plus tissue embedding, pleura plus remaining-pulmonary resection or thoracoplasty.

病人分别采取胸腔闭式引流、胸腔冲洗、经纤维支气管镜生物胶封堵、瘘口修补加组织包埋、胸膜余肺切除、胸廓成形术等方法治疗。

Methods: For 66 cases whose bone marrow showed hypocellularity in BM aspirate smear, trephine biopsy and reticulin staining were done at the same location in the same patient and at the same time as the BM aspirate smear was done.

对66例骨髓涂片显示细胞增生低下患者的同期同部位骨髓活检组织用塑料包埋半薄切片，做常规染色及网状蛋白染色，光镜下观察。

Methods One hundred and eight Sprague-Dawley rats were divided into three troops: the troop of flaps, the troop of grafts and the troop of fat with thirty six of each.

将带有PCD-VEGF165的大肠杆菌接种到LB培养基中，通过碱分裂法制备PCD-VEGF165质粒，再用反蒸发法将质粒包埋于脂质体中。

Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method; ERα and ERβ distribution were investigated in uvea tissue of rats; and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control.

选择青春期SD雌性大鼠22只，采用颈椎脱臼处死大鼠，取眼球作常规石蜡包埋，连续切片， SP免疫组织化学方法显示ERα、ERβ在葡萄膜组织的表达；采用Tanaka记分法对ERα、ERβ进行定量分析，并设正常大鼠子宫作阳性对照；PBS代替一抗作阴性对照。

Objective To explore the feasibility and application of laparoscopic appendectomy by silk suture with three trocars.Methods The root of vermiform appendix was stitched up and enwrapped in laparoscopic appendectomy by silk stitches.

目的 探讨三孔缝合打结法在腹腔镜阑尾切除术中的可行性及应用方法在腹腔镜阑尾切除术中，应用三孔丝线缝合及结扎的方法处理阑尾根部、包埋阑尾残端。

Methods Biopsy samples of were obtained from 353 patients with cervical carcinoma, HPV were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18 were found in 138 (39.1%) 353 cervical carcinoma, while HPV types 6, 11 were found in 81 (69.2%) of 117 acuminate verruca.

采用聚合酶链反应-核酸内切酶分型检测宫颈癌活检组织中HPV-DNA，对来源于子宫颈癌患者的石蜡包埋组织标本进行四种常见生殖道人乳头瘤病毒(HPV6、11、16、18)的检测和分型。

Vitrification and encapsulation_dehydration are two new techniques and may h...

由于两步法保存技术的局限性，玻璃化和包埋脱水法等新技术在某些藻类的种质保存中可能有较大的应用潜力。

Effects of different temperatures on zein film were evaluated for release of nisin into hydrochloric acid.

研究了4种不同温度对玉米朊包埋乳酸菌素释放的影响。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。