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Objective To study antioxidation ofDi-(3β-hydroxyandrost-5-en-17-one) ester, a new compound which was structure derivated from active substance from Asterias amurensis.

目的 以兔和大鼠心肌及肝组织匀浆为材料,研究一种从多棘海盘车中提取得到的活性物质经结构改造和修饰得到的丁二酸二-(3β-羟基雄甾5-烯-17-酮)酯新化合物抗脂质过氧化作用。

objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.

目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验,取50只小鼠随机分成5组,分别给予溶媒和不同剂量的色素,然后采用溴代苯致实验性肝损伤,测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用,抑制率随样品的浓度增高而增大,并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中,中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。

The findings of this study demonstrate the presence of enzymes in S. Japonicum, which are able to catabolize the heme.

成虫匀浆抽提物体外降解血红素的反应对pH值有依赖性,HO和BR反应的最佳pH环境均为pH 8.7。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测:将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心,取上清液,按Blanckaert法测定胶原酶,所得据经统计学处理差异无显著性。

Methods 60 female mice were randomly divided into 4 groups (n=15):Mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of D-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 D-半乳糖[1000 mg/] 造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides [200 mg/group,tanshinone [1 500 mg/] group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

60只雌性昆明种小鼠,随机分为4组(n=15):正常对照组皮下注射生理盐水;其余3组每日颈背部皮下注射 d-半乳糖[1000 mg/]造成小鼠衰老模型,同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后,测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

LDL??C, ALT and AST in serum, and the even plasma TG and CHOL contents.

、LDL??C、ALT、AST水平及肝组织匀浆TG、CHOL的含量。

The pharmacokinetics of Hup A in blood and eerebrospinal fluid after intranasal, intravenous and intragastric adminstration to rats was studied using cisternal cannulation for serial CSF sampling and femoral artery cannulation for serial blood sampling.

以市售片剂和注射液为对照,用小脑延髓池插管法采集脑脊液,股动脉插管取血,测定其在大鼠脑脊液和血中药物动力学参数;用组织匀浆法,测定其在大鼠脑组织中的分布;用Morris水迷宫法、跳台法和避暗法试验其对大鼠和小鼠模型的药效。

Colorimetric method was used to determine PGE and MDA contents.

采用比色法分别测定了大鼠血浆中PGE含量及肝脏匀浆中MDA的含量。

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