匀浆

Methylmercury was extracted from the homogenized tissue with toluene in the presence of copper sulfate, sodium bromide and sulfuric acid

在存在硫酸铜、溴化钠和硫酸的情况下，用甲基从组织匀浆中萃取甲基汞。

Experiments were conducted with fertile chicken eggs to determine the effects ofthyroxine and thiouracil on chicken digestive enzyme activity,hatchability and yolk-absorption.

分别测定孵化第17d、第19d及1、5、10、15、20日龄小鸡的腺胃、十二指肠与胰腺匀浆消化酶的活性，检查啄壳迟早、孵化率与雏鸡卵黄囊吸收情况。

Following the treatment with Trillium Tschnoskii Maxim,ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treating group.

结果 头顶一颗珠能明显降低CCl4致小鼠肝损伤血清ALT、AST值升高，降低肝组织匀浆中MDA的含量，增强SOD的活性。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

Superior mesenteric artery was clipped for 60 min to cause ischimia, and then unclipped to make reperfusion for 2 hours after reperfusion, the liver tissues were gathered and homogenized respectively, and the contents of SOD, GSHPX, MDA and ATPase,and morphological changes of hepatocytes were assayed.

夹闭大鼠肠系膜上动脉60 min造成缺血，再灌注2 h后取出肝组织制成匀浆，测定超氧化物歧化酶、谷胱甘肽过氧化酶、丙二醛、Ca2+Mg2+ATP酶的含量及肝形态细胞学变化。

Superior mesenteric artery was clipped for 60 min to cause ischimia,and then unclipped to make reperfusion.2 hours after reperfusion,the renal tissues were gathered and homogenized,and the contents of SOD,GSHPX,MDA and Ca2+Mg2+ATPase,and morphological changes of renal were assayed.

夹闭大鼠肠系膜上动脉60min造成缺血，再灌注2h后取出肾组织制成匀浆，测定超氧化物歧化酶、谷胱甘肽过氧化酶、Ca2+Mg2+ATPase的活性及丙二醛含量，观察肾组织形态细胞学变化。

METHODS: A total of 30 neonatal rats were selected to prepare burn models by giving 90 ℃ boiled tap water for 3 seconds, and vibrissa follicle bulge was separated at 12, 24, 36, 48 and 60 hours postoperatively. The remained 6 neonatal rats were served as controls.

随机选取30只乳鼠，采用90 ℃水温持续3 s时间的方式制作大鼠触须部表皮组织浅Ⅱ度烫伤模型，造模后第12，24，36，48，60小时对毛囊隆突区进行分离，并进行匀浆，以同窝新生6只乳鼠正常唇部组织作为对照。

It was helpful to extract enzyme by using homogenate in citrate-phosphate buffer; The enzyme activity extracted from pH5.6 buffer is higher than that of pH5.8.The loss of enzyme activity of this zymin is better, but easily used in manufacture.

丙酮粉提取法会引起回收酶活性较大损失，其总酶活仅是缓冲液匀浆法和浸提法的1/5、1/7，但酶制剂本身的酶活性较好，使用方便，有利于工业化生产应用。

Methods Hyperlipemia model rats were established by feeding the rats with high fat di- et,meanwhile they were given 50,100 and 200 mg.kg~(-1) Thelenata anax and Apostichopus japonicus for 30 days.

方法采用高脂饲料饲喂法建立高脂血症大鼠模型，同时分别灌胃不同剂量(50、100和200mg／kg)的北极刺参与日本刺参匀浆，30d后测定大鼠血清TC，TG。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。