匀浆

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片，将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片，用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS，并观察该酶在各期虫体内的分布；用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

Methods A model of acute renal failure was established by injecting glycerin muscularly and HgCl_2 subcutaneously in Wistar rats.

本实验选用肌肉注射甘油和皮下注射HgCl2复制出大鼠ARF模型，观察NO、ET-1在ARF大鼠血浆、肾组织匀浆中的含量变化，探讨两者与ARF中肾脏损害机

Objective: To detecti the value of SOD and MDA in adriamycin nephrosis rat.

目的：探讨阿霉素肾病大鼠肾组织匀浆超氧化物岐化酶和丙二醛水平及其临床意义。

Acquisition of high-quality chromosome suspension: The chromosome suspension released from homogenization suited for flow karyotypic analysis and sorting after fixed in 2% paraformaldehyde for 20 minutes and purified through 10% and 30% sucrose concentration gradient.

高质量染色体悬液制备结果：同步化的根尖细胞经2%多聚甲醛固定20 min，机械匀浆释放染色体并结合10%及30%蔗糖浓度梯度纯化释放的染色体悬液。

AIM To investigate the effect of methyl pentyl 1,4 dihydro 2,6 dimethyl 4 (3 nitrophenyl) 3,5 pyridinedicarboxylate (MN9202),a dihydropyridine calcium antagonist, on cardiac indexes,ultrastructure, superoxide dismatase,glutathione peroxidase activity and malondialdehyde concentration in cardiac hypertrophy induced by L thyroxine in rats.

连续 12 d ip L- Thy0 。5 mg·kg- 1 · d- 1 复制大鼠心肌肥厚模型，实验组 ip MN92 0 2 0 。4mg· kg- 1· d- 1 ，2 mg·kg- 1· d- 1 ，10 mg· kg- 1· d- 1 ，阳性对照组 ip卡托普利 2 5 mg· kg- 1 · d- 1 ，模型组、对照组给予等量溶剂，于给药后的第 13日处死大鼠，检测心脏指数，电镜观察心肌组织的超微结构变化，化学比色法测定心肌匀浆中 SOD、GSH- PX活性和 MDA含量。

Methods: Prepared the epilepsy model of rat with picrotoxin injection, then it was interfere the model with immunoglobulin injection either before or after seizure respectively.

用印防已毒素制备癫痫大鼠模型，在发作前、后分别给予IVIg进行干预处理，检测脑组织匀浆内TNF-α和IL-2、4、10的浓度变化，并与对照组比较。

It is concluded that pulverize of tissue in liquid nitrogen remains the integrity of RNA isolated and may be applied for RNA isolation from human embryonic tissues.

液氮研磨方法可以应用於胚胎组织匀浆分离完整RNA，而不同引产方式对胚胎组织RNA质量并无直接的影响。

At the end of feeding for 2, 4, 6 and 8 wk, 6 rats in each group were randomly selected, the serum fasting blood sugar, fasting insulin and the fasting insulin resistance index, and the concentration of IL-18 in serum and liver tissue serosity were measured.

将48只♂SD大鼠随机分为正常饮食组及高脂饮食组，各24只，喂养至第2、4、6、8周末时， 2组各随机抽取6只，抽取门静脉血测空腹血糖、空腹胰岛素，计算空腹胰岛素抵抗指数；检测血清及肝组织匀浆IL-18浓度；光镜检查肝组织切片以评价肝脏脂肪变性程度。

Acute liver injury model in mice was made by using CCl4.The levels of ALT, AST, albumin and globulin in serum and MDA, SOD in liver and hepatic pathological tissue were observed.

基于CCl4皮下注射制备肝损伤小鼠模型，分别用3种不同浓度的魔芋葡甘聚糖进行干预，观察检测小鼠血清谷丙转氨酶、谷草转氨酶、血清白蛋白与血清球蛋白的比值、肝组织匀浆超氧化物歧化酶和丙二醛含量。

Objective:The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterasein cerebral homogenate and blood serum of rats.

中文摘要：目的：考察中药单体芥子碱(3，5-二甲基，4-羟基苯丙烯酰胆碱，sinapine)对大鼠血清和大鼠大脑匀浆乙酰胆碱酯酶(acetylcholinesterase，AChE)的体外抑制作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。