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In this method, ethyl acetale and dichloromethane were used to extract residues with eddy-oscillate method.

用二氯甲烷,采用匀浆法提取,并用安捷伦的C18柱(500 mg;6 mL)进行净化。

It can be measured the ability of learning memory by shuttle box. The SYN of hippocampus and cortex neurons were observed by electron lens using immunohistochemistry method.

运用穿梭箱检测各组大鼠的学习记忆能力,采用生化法检测各组大鼠脑匀浆AchE,免疫组化方法观察SYN蛋白的变化。

METHODS: The normal hippocampi and hippocampi on the 14th day after the hippocampal fimbria transection were prepared into homogenate used for 10% native- polyacrylamide gel electrophoresis, and the differential proteins of 83 ku were electroeluted. The protein concentration was adjusted to 300 mg/L.

取6只正常大鼠及6只切割海马伞后14 d大鼠的海马组织制成匀浆,进行非变性聚丙烯酰胺凝胶电泳,根据染色结果切取含有83 ku差异蛋白条带进行电洗脱,定量后调整蛋白浓度为300 mg/L。

The factors included the type of extractant, the amount and the extraction times, the type of purified column, the type and dosage of eluviatant about the pre-treatment of the samples. The extraction was 3 minutes (13500rpm) of high speed homogenize extraction. 50mL acetonitrile as an extractant was acted on one time extraction. The florin silicon column was taken as the purified column. The extraction was eluted by 30ml 3:2 acetone - hexane and concentrated by nitrogen blowing under 50 °C water bath.The chromatographic separated conditions were studied applied the Agilent 6890 gas chromatographic apparatus and DB-17 capillary column(30m×0.25mm×0.25μm).

研究了提取剂种类、用量与提取次数,净化柱种类,淋洗剂种类与用量,提取方式与提取时间,柱温和柱流量等因素对油菜籽中有机磷农药残留提取、分离净化和色谱分离的影响,建立了采用高速匀浆提取方式提取3min13500rpm.min~(-1,以50mL乙腈为提取剂一次提取,以弗罗里硅柱为样品液净化柱,以30mL 3:2丙酮-正己烷为淋洗剂,在50℃水浴条件下用氮吹浓缩,气相色谱检测的油菜籽有机磷农药多种残留检测技术。

Methods Established the Wistar rat models of EAE by footpad injection of guinea pig spinal cord homogenates and CFA. Animals were executed 6 days after disease onset, and optic nerves, brains and spinal cords were removed for light and electron microscope pathological analysis.

足垫皮下注射豚鼠脊髓匀浆和完全弗氏佐剂混合物制作Wistar大鼠EAE模型,于发病后第6 d将大鼠处死,取视神经、脑和脊髓,行HE和LFB染色,光镜和电镜下观察其病理改变。

MSCs induced by spinal cord homogenate supernatant can be transplanted into hemisection spinal cord injury and improve the motor function of the injuries lesions.

MSCs经脊髓匀浆上清诱导后移植治疗大鼠半横断脊髓损伤可使运动功能得到改善。

The water buffalo and rat were chosen to study and discuss changes and action mechanism of some arachidonic acid metabolites in liver tissue homogenate by mean of biological and chemical liver injuries induced with F. hepatica and CCl4, respectively.

以水牛、大鼠为对象,采用感染肝片吸虫的生物性和由CCl4导致的化学性致肝脏损伤两种方法,探讨了肝损伤组织匀浆中某些花生四烯酸代谢物的水平变化。

Objective To investigate the effects of myocardial homogenate of rats on transdifferentiation of muscle satellite cells.

目的:探讨心肌匀浆上清液对大鼠骨骼肌卫星细胞诱导分化的影响。

ET1 content in lung homogenate get to the peak at 2 h and then started to decrease.

统计分析表明匀浆和血浆各自ET1的均数在转流前后差异有统计学意义。

In renal cortex homogenate, the contents of malonaldehyde and activity of superoxide dismutase were measured.

肾皮质制备匀浆检测丙二醛含量和超氧化物歧化酶活性。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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