匀浆

Methods Forty-eight healthy black and blue rabbits were chosen and divided into 6 groups at randomly. Experimental groups were performed surgery with CO2 laser sclerectomy in both eyes. Contents of nitric oxide and malonyldialohyed and the activity of Na(superscript +)-K(superscript +)-ATPase were tested with biochemical assays.

健康青紫兰兔48只，随机分为6组，实验组动物双眼均接受CO2激光外路巩膜切除术，化学比色法测定视网膜匀浆中一氧化氮、丙二醛的含量和Na-K-ATP酶的活性，进行统计学分析。

Naphthylamide heavy nitrogen spectrophotography was adopted to detect the content of nitric oxide in the serum and half quantitative reverse transcription polymerase chain reaction was used to detect eNOS gene expression in artery vessels.

采用萘胺重氮分光光度法测定血清NO含量，采用半定量RT PCR方法检测各组动物动脉壁匀浆中eNOS基因的表达，分析造模及各药物组血清NO含量及eNOS基因表达的变化。

Next we used striatal extracts as revulsant to do inductive experiments of ESCs.

将纹状体组织匀浆，制备纹状体提取液，并以此进行ESC的诱导分化实验。

After gestational periods the activities of SOD,MDA,GSH-Px,TChE and CAT in brain were analyzed respectively.

采用饮水染毒，染毒时间为21d，分娩后每窝随机选取6只乳鼠测定脑匀浆超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乙酰胆碱酯酶活性和丙二醛的含量。

After 5 days we began to measure the abilities of learning and memory in the Morris water maze. The avoiding latency, the frequency of passing the platform and the stay time in the aim quadrant were determine in mice. The activities of TchE and MAO in the cerebra of mice were detected.

第5d后进行Morris水迷宫测试，测试期间保持音乐刺激不变，放完音乐后测试潜伏期、经过平台次数和平台象限停留时间，实验结束后处死小鼠取脑组织并匀浆，检测乙酰胆碱酯酶活性和单胺氧化酶活性。

The therapeutic effect of HYJDT on glomerulosclerosis was observed by way of five aspects:(1) Effect on renal tissue morphology;(2) Effect on urine protein and blood creatinine and blood uria nitrogen ;(3) Effect on superoxide dismutase and malonydialdehyde in reual cortex;(4) Effect on matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-l (TIMP-1) in glomerulus;(5) Effect on MMP-9 mRNA and TIMP-lmRNA and type-1 plasminogen activator inhibitor (PAI-1) mRAN in glomerulus.

用药治疗12周取肾，HE、PAS染色，在光镜下观察肾组织形态学变化，并在透射电镜下观察肾小球超微结构的变化。用半自动生化分析仪检测肾皮质匀浆中SOD活力、MDA含量。用免疫组化技术检测肾小球中MMP-9、TIMP-1的蛋白表达，用原位杂交技术检测MMP-9mRNA、TIMP-lmRNA、PAI-lmRNA的基因表达。免疫组化和原位杂交结果，采用计算机图象分析系统测量各自的平均吸光度。所有的实验数据均用SAS6.12统计软件包进行统计学处理。

The most efficient method was selected and applied to RNA extraction from clinical samples of patients with vulvovaginal candidiasis.

采用人体上皮细胞与念珠菌标准菌株的混合物（106 ∶ 106），对匀浆、玻璃珠和液氮研磨三种破壁方法，纯化柱、酚两种提取方法组合产生的5种不同RNA提取方法进行比较。

Objective:To observe the effect of the extract of Carbonized Herba Schizonepetae on PGE content in the rats'plasma and MDA content in the liver.

目的：观察荆芥炭提取物对大鼠血浆中PGE含量及肝脏匀浆中MDA含量的影响。

Then the 10% cerebrums inhomogenate was used to test the content of monoamine oxidase, superoxide dismutase and acetylcholinesterase.

实验中测试大鼠的应激记忆力，测定脑匀浆中MAO，SOD，AchE、MDA的含量以及对大脑进行组织学检查。

Fresh carrot, tomato and chili were used as test samples, absolute alcohol were used in process of triturating and dehydrating, acetone were used for extraction, all of the alcohol solution and acetone solution were commixed, and the content of β-carotene were directly determined by HPLC.

选用胡萝卜、番茄和红辣椒三种常见新鲜果蔬作为供试品，采用无水乙醇匀浆、脱水，丙酮提取-直接定容的方法制备供试品溶液，使用高效液相色谱法测定供试品中β-胡萝卜素的含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。