匀浆

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

Results Compared with the model group, crocetin can significantly reduce the cardiac indexes, obviously decrease the level of MDA, LDH and CK in the serum. Crocetin can significantly increase the activities of Na +, K +-ATPase; Ca 2+, Mg 2+-APTase; GSH-Px. The histopathological changes confirmed the protective effects of crocetin on the myocardial injury.

结果 西红花酸能显著降低血清中CK和LDH的释放；也能显著降低血清和心脏匀浆中MDA水平，心脏重量参数显示对心肌水肿有明显的抑制作用；保护心肌GSH—Px；Na^ ，K^ —ATP酶和Ca^2 ，M8^2 -ATP酶活性，心脏病理切片表明西红花酿对心肌坏死有显著的保护作用。

A virus strain with high hemagglutinin titer was isolated out from embryonated egg and MDCK cells after they were inoculated with the lung tissues of the died marmosets.

在排除细菌感染的基础上，通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养，分离出一株具有很高血凝效价的病毒株。

A virus strain with high hemagglutinin titer was isolated out from embryonated eggs and MDCK cells after inoculating with the lung tissues suspension of the dead marmosets.

在排除细菌感染的基础上，通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养，分离出一株具有高血凝效价的病毒株。

Methods The distribution of Pomacea canaliculata, Achatna fulica and slugs was investigated in fields, farms, restaurants and free markets. Lung-check up and organize-plasm were used in the infection inspection of A. cantonensis.

分别在野外、螺类养殖场、餐饮场所、自由市场等4类场所进行福寿螺、褐云玛瑙螺、蛞蝓等中间宿主的分布调查，应用&肺检查法&结合&组织匀浆法&进行广州管圆线虫感染调查。

Blood and CSF of patients were collected for syphilis,toxoplasma gondii and routine,cytological, glycose,protein,chloride, virology examination and 14-3-3 protein Western Blot of CSF were were carried out too.

采集患者血液和CSF，进行CSF常规及细胞学、糖、蛋白质和氯化物；血液和CSF病毒学、弓形体、梅毒检测；CSF 14-3-3蛋白Western Blot检测。2例死亡患者脑组织匀浆进行Wistar大鼠脑内接种，观察期6个月。

The results indicated that the water or 50% ethanol extracts from peonies petals could reduce light absorption of DPPH and OH initiated hydroxylation of salicylate, and inhibit O2(superscript -) mediated light chemical reduction of nitroblue tetrazolium effectively, and lipid peroxidation of polyunsaturated fatty from yolk lipoprotein, in which DPPH scavenging activities of 6 peonies water extracts were hinger than that of 200 μg/mL BHT, but lower than 80 μg/mL Vc. In 4 kinds of detecting systems of antioxidation, the peroxidation activity of deep colored flowers of peonies was higher than that of light colored flowers of peonies.

牡丹花瓣提取液能明显抑制卵黄组织匀浆的脂质过氧化作用，降低DPPH的吸光值、抑制O2介导的NBT光化学还原及OH作用下的水杨酸羟基化作用，其中牡丹花瓣提取液清除DPPH自由基的活性高于200μg/mL BHT，但低于80μg/mL Vc.6个品种的牡丹花瓣提取液，在4种测定系统中均具有一定程度的抗氧化能力，且水提取液的抗氧化活性略高于体积分数50%乙醇提取液，深色花瓣提取液对不同自由基的清除能力较强。

Lung histomorphological changes were observed by light microscope.

实验评估：酶联免疫吸附法检测各组动物血液、肺组织匀浆及肺泡灌洗液中的白细胞介素6、白细胞介素8和肿瘤坏死因子α含量；光镜下观察肺组织形态学变化。

The histomorphological changes of rat brain were examined by electronmicroscope.

给大鼠应用DPH，用荧光分光法检测其在低压舱模拟升空7.5 km后1 h、2 h的脑匀浆单胺递质含量，同时观察脑细胞形态学改变。

Hours later,these mice were decapitated,and the striatums were isolated from thebrains. The levels of dopamine, serotonin ( 5-HT ), homovanillic acid, 5-hydroxyindoleacetic acid ( 5-HIAA ) andnorepinephrinein the striatum were measured by high-performance liquid chromatography with electrochemical detection.

给3组小鼠分别喂食Dieldrin 50 mg·kg-1体重、Dieldrin 40 mg·kg-1体重和等量的生理盐水，3 h后处死小鼠，取纹状体匀浆用高效液相色谱-电化学检测法测定纹状体中多巴胺、5-羟色胺(5-HT)、高香草酸、5-羟基吲哚乙酸(5-HIAA)和去甲肾上腺素的含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。